营养盐对假微型海链藻中鞘脂合成的影响
PDF下载 (256)黄莉莉,陈文笔,李艳荣,徐继林,陈海敏,朱竹君,周成旭,严小军.营养盐对假微型海链藻中鞘脂合成的影响[J].宁波大学学报(理工版),2016,29(3):23-28.DOI:
HUANG Li-li,CHEN Wen-bi,LI Yan-rong,XU Ji-lin*,CHEN Hai-min,ZHU Zhu-jun,ZHOU Cheng-xu,YAN Xiao-jun.Effect f Nutrient on the Sphingolipid Synthesis of Thalassiosira pseudonana[J].Journal of Ningbo University(Natural Science & Engineering Edition),2016,29(3):23-28.DOI:
| Title: | Effect f Nutrient on the Sphingolipid Synthesis of Thalassiosira pseudonana |
| 作者: | 黄莉莉, 陈文笔, 李艳荣, 徐继林, 陈海敏, 朱竹君, 周成旭, 严小军 |
| Author(s): | HUANG Li-li, CHEN Wen-bi, LI Yan-rong, XU Ji-lin*, CHEN Hai-min, ZHU Zhu-jun, ZHOU Cheng-xu, YAN Xiao-jun |
| 关键词: | 假微型海链藻; 营养盐; 鞘脂; 丝氨酸棕榈酰转移酶 |
| Keywords: | Thalassiosira pseudonana; nutrient; sphingolipid; serine palmitoyltransferase |
| 分类号: | Q542; Q555 |
| 文献标识码: | A |
| 摘要: | 丝氨酸棕榈酰转移酶(Serine Palmitoyltransferase, SPT)是鞘脂合成途径中的关键酶, 其表达水平的高低影响生物体内鞘脂的含量. 该实验利用荧光定量PCR和液相色谱―质谱技术分析了营养盐对假微型海链藻中SPT表达及鞘脂合成的影响. 结果表明: SPT在微藻细胞中的表达水平直接影响其鞘脂的合成, 且P饥饿促进SPT的表达和细胞内神经酰胺的合成, 进而影响假微型海链藻的生长; 而N饥饿抑制SPT的表达和细胞内神经酰胺的合成及假微型海链藻的生长. |
| Abstract: | Serine palmitoyltransferase (SPT) is the key enzyme in the de novo synthesis of sphingolipid, and its expression level can affect the content of sphingolipid in cells. In this experiment, the effect of nutrient on the expression of SPT and the synthesis of sphingolipid in Thalassiosira pseudonana is investigated using real-time PCR and ultra performance liquid chromatography mass spectrometry. Results indicate that the expression level of SPT affects sphingolipid content in microalgae cells. Moreover, phosphorus starvation makes both the expression level of SPT and the synthesis of ceramides in cell increase, which affects the growth of T. pseudonana. In contrast, the nitrogen deprivation inhibites both the expression of SPT and the synthesis of ceramides in cell, and significantly restrains the growth of T. pseudonana. The results are expected to provide theory foundation for some studies of the growth, decay and metabolism of T. pseudonana. |
| 参考文献 /References: | [1].刘圣红, 苟萍. 鞘脂类的研究进展[J]. 生物技术, 2009, 19(2):96-97. [2].刘小平, 苟萍. 鞘脂合成的中间代谢酶类的研究进展[J]. 科技信息, 2008(16):7-8. [3].IKUSHIRO H, HAYASHI H, KAGSMIYAMA H. A water-soluble homodimeric serine palmitoyltransferase from Sphingomonas paucimobilis EY2395T strain purification, characterization, cloning, and overproduction [J]. Journal of Biological Chemistry, 2001, 276(21):18249- 18256. [4].NAGIEC M M, BALTISBERGER J A, WELLS G B, et al. The LCB2 gene of Saccharomyces and related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis[J]. Proceedings of the National Academy of Sciences, 1994, 91(17): 7899-7902. [5].WEISS B, STOFFEL W. Human and murine serine- palmitoyl-CoA transferase cloning expression and characterization of the key enmyze in sphingolipid synthesis[J]. European Journal of Biochemistry, 1997, 249(1):239-247. [6].IKUSHIRO H, ISLAM M M, TOJO H, et al. Molecular characterization of membrane associated soluble serine palmitoyltransferases from Sphingobacterium multivorum and Bdellovibrio stolpii[J]. Journal of Bacteriology, 2007, 189(15):5749-5761. [7].GABLE K, HAN G, MONAGHAN E, et al. Mutations in the yeast LCB1 and LCB2 genes, including those corresponding to the hereditary sensory neuropathy type I mutations, dominantly inactivate serine palmitoyltransferase [J]. Journal of Biological Chemistry, 2002, 277(12): 10194-10200. [8].HANADA K. Serine palmitoyltransferase, a key enzyme of sphingolipid metabolism[J]. Biochimica Biophysica Acta (Molecular and Cell Biology of Lipids), 2003, 1632(1):16-30. [9].刘旭宏. 海洋球石藻病毒(Coccolithovirus)丝氨酸棕榈酰转移酶(SPT)基因克隆表达及其与宿主神经酰胺合成之间的关系[D]. 集美大学, 2012. [10].杨秀霞, 于浩, 曾哓起. 影响微藻脂肪酸组成因素概述[J]. 海洋湖沼通报, 2001(1):76-82. [11].YONGMANITCHAI W A R D, WARD O P. Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions[J]. Applied and Environmental Microbiology, 1991, 57(2):419-425. [12].ZHILA N O, KALACHEVA G S, VOLOVA T G. Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus[J]. Journal of Applied Phycology, 2005, 17(4):309-315. [13].DELAJARA A, MENDOZA H, MARTEL A, et al. Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodini umcohnii[J]. Applied Phycology, 2003, 15(5):433-438. [14].ARMBRUST E V, BERGES J A, BOWLER C, et al. The genome of the diatom Thalassiosira pseudonana: Ecology, evolution, and metabolism[J]. Science, 2004, 306(5693):79-86. [15].GUILLARD R R L. Culture of phytoplankton for feeding marine invertebrates[C]//Smith W, Chanley M H. Culture of Marine Invertebrate Animals, New York: Plenum Press, 1975:29-60. [16].赵方. 几种海洋硅藻中鞘脂的液质分析及其结构规律[D]. 宁波: 宁波大学, 2013. [17].孙凯峰, 胡章喜, 段舜山. NP 营养盐胁迫对两株布朗葡萄藻生长的影响[J]. 水生生物学报, 2010, 34(3): 517-524. [18].张冬鹏, 武宝玕. 几种赤潮藻对温度、氮、磷的响应及藻间相互作用的研究[J]. 暨南大学学报自然科学版, 2000, 21(5):82-87. [19].石岩峻, 胡晗华, 马润宇, 等. 塔玛亚历山大藻对氮和磷的吸收及其生长特性[J]. 应用生态学报, 2003, 14(7): 1143-1146. [20].袁美玲, 王朝晖, 李友富. N、P营养盐对海洋卡盾藻(Chattonella marina)生长的影响[J]. 生态学报, 2008, 28(1):430-435. [21].王勇, 焦念志. 营养盐对浮游植物生长上行效应机制的研究进展[J]. 海洋科学, 2000, 24(10):30-33. [22].JUSTIC D, RABALAIS N N, TURNER R E, et al. Changes in nutrient structure of river-dominated coastal waters: Stoichiometric nutrient balance and its consequences[J]. Estuarine, Coastal and Shelf Science, 1995, 40(3):339-356. [23].VAN MOOY B A, FEWDRICKS H F, PEDLER B E, et al. Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity[J]. Nature, 2009, 458:69-72. [24].蒲云峰, 张伟敏, 钟耕. 神经酰胺功能和应用[J]. 粮食与油脂, 2005(7):14-16. |
| 备注/Memo: | 收稿日期: 2015?11?04. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（31172448）; 教育部博士点基金优先发展领域项目（20133305130001）; 浙江省公益性项目（2014C32081）; 浙江省自然科学基金（LY15C190004）; 宁波市科技攻关项目（2013C10014, 2014C10005）. 第一作者: 黄莉莉（1989－）, 女, 河南商丘人, 在读硕士研究生, 主要研究方向: 微藻生物化学. E-mail: 1263623801@qq.com *通信作者: 徐继林（1965－）, 男, 浙江宁波人, 研究员, 主要研究方向: 微藻生物化学. E-mail: xujilin@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
