Fe3O4@PEG磁性纳米粒子合成及其对阿霉素的载药性能
PDF下载 (22625)张坤1,江峰1,季帆1,谷元亮2,苏红2,赵进顺2,张剑锋1*.Fe3O4@PEG磁性纳米粒子合成及其对阿霉素的载药性能[J].宁波大学学报(理工版),2018,31(3):60-64.DOI:
ZHANG Kun1,JIANG Feng1,JI Fan1,GU Yuan-liang2,SU Hong2,ZHAO Jin-shun2,ZHANG Jian-feng1*.Magnetic Fe3O4@PEG nanoparticles synthesis and application for doxorubicin delivery[J].Journal of Ningbo University(Natural Science & Engineering Edition),2018,31(3):60-64.DOI:
| Title: | Magnetic Fe3O4@PEG nanoparticles synthesis and application for doxorubicin delivery |
| 作者: | 张坤1, 江峰1, 季帆1, 谷元亮2, 苏红2, 赵进顺2, 张剑锋1* |
| Author(s): | ZHANG Kun1, JIANG Feng1, JI Fan1, GU Yuan-liang2, SU Hong2, ZHAO Jin-shun2, ZHANG Jian-feng1* |
| 关键词: | 阿霉素; 纳米粒子; 四氧化三铁; 药物修饰; 聚乙二醇化 |
| Keywords: | doxorubicin; nanoparticles; ferriferous oxide; drug modification; pegylation |
| 分类号: | TQ460.1 |
| 文献标识码: | A |
| 摘要: | 将通过化学共沉淀方法得到的Fe3O4纳米粒子依次用柠檬酸和双羧基化的聚乙二醇进行修饰, 合成了具有磁靶向的纳米粒子载体(Fe3O4@PEG), 分别用X射线衍射、红外光谱、扫描电镜、粒径分布、热重分析对其结构进行了表征, 将合成的磁性纳米粒子负载上模型药物阿霉素(DOX), 得到新的载药体系Fe3O4@PEG-DOX, 研究了该载药体系的载药特征和释放行为. 研究发现, 该载体在水中载药率为85%, 累积释放实验表明, 在72h内, pH 5.0时释放率达到71%, 远高于pH 7.4时43%的释放率, 体外细胞实验表明负载阿霉素的磁性纳米载体的抗肿瘤活性没有损失, 与未经修饰的阿霉素的抗肿瘤活性相当. 研究结果表明, 通过柠檬酸连接的Fe3O4@PEG纳米粒子对阿霉素具有较好的修饰作用, 是一种较为理想的阿霉素载药系统. |
| Abstract: | Magnetic Fe3O4@PEG nanoparticles were prepared by modification of Fe3O4 with citric acid and di-carboxylic polyethylene glycol (PEG). The structure of magnetic nanoparticles was characterized by XRD, FT-IR, SEM, PSD, TGA. Doxorubicin (DOX) was loaded by the magnetic Fe3O4@PEG nanoparticle to establish a novel drug delivery system (DDS), Fe3O4@PEG-DOX. The loading capacity of nano-carrier and the release behavior of Fe3O4@PEG-DOX was studied. The results reveal that the loading capacity of the nano-carrier is 85%. The cumulative release test reveals that the drug release rate reaches to 71% in pH 5.0 PBS buffer by contrast a lower drug release rate of 43% in pH 7.4 with 72h. In vitro experiments indicate that Fe3O4@PEG- DOX shows the same antitumor activity as the unmodified doxorubicin without significant loss of cytotoxicity. The research forecasts that citric acid coated Fe3O4@PEG nanoparticles is a prospective DDR system for doxorubicin. |
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| 备注/Memo: | 收稿日期: 2017?10?09. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省公益性技术应用研究计划（2015C31044）; 宁波市自然科学基金（2014A610113）. 第一作者: 张坤（1993－）, 女, 安徽合肥人, 在读硕士研究生, 主要研究方向: 生物医药材料. E-mail: zhangzkkun@126.com *通信作者: 张剑锋（1968－）, 男, 湖南邵阳人, 教授, 主要研究方向: 材料化学. E-mail: zjf@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
