快速启温好氧堆肥过程中关键酶活性变化规律研究
PDF下载 (311)徐 超,李 兵,俞雯雯,岳 远,董志颖.快速启温好氧堆肥过程中关键酶活性变化规律研究[J].宁波大学学报(理工版),2019,32(2):16-22.DOI:
XU Chao,LI Bing,YU Wen-wen,YUE Yuan,DONG Zhi-ying.Study on variations of key enzyme activity in quick start-up aerobic composting process[J].Journal of Ningbo University(Natural Science & Engineering Edition),2019,32(2):16-22.DOI:
| Title: | Study on variations of key enzyme activity in quick start-up aerobic composting process |
| 作者: | 徐 超, 李 兵, 俞雯雯, 岳 远, 董志颖 |
| Author(s): | XU Chao, LI Bing, YU Wen-wen, YUE Yuan, DONG Zhi-ying |
| 关键词: | 餐厨垃圾; 太阳能; 好氧堆肥; 酶活性 |
| Keywords: | kitchen waste; solar energy; aerobic composting; enzyme activity |
| 分类号: | X706 |
| 文献标识码: | A |
| 摘要: | 实验利用一套自行设计的水浴式供能好氧堆肥系统, 以典型有机垃圾-餐厨垃圾为原料进行了3批堆肥处理试验, 第1批实验利用太阳能供能, 第2批利用太阳能辅助电能供能, 第3批无任何外加能量, 研究了此3种供能堆肥过程中物质降解与关键性酶的活性变化规律. 实验结果显示: (1)第1批受外界天气影响较大, 堆温变化波动幅度大, 最高堆肥只有43℃, 仅为中温堆肥; 第2批实验最高堆温可达56℃, 高温堆肥期有4d; 第3批实验完全为常温堆肥, 反映出外界环境温度的影响较大; (2)第2批实验最终的C/N值为10.2, 低于第1批的12.1与第3批的13.7, 表明第2批实验中物料降解更彻底; (3)第2批实验过程中, 过氧化氢酶、脲酶、蛋白酶的活性均高于第1批与第3批实验, 表明第2批实验过程中的生化反应更剧烈. 其中, 过氧化氢酶和脲酶活性在初期较高, 堆体温度上升后迅速下降, 并保持在较低水平; 蛋白酶活性初期较高, 随堆温的升高而上升, 反映出在堆肥的不同阶段发挥主导作用的酶活性不同. |
| Abstract: | In the experiment a self-designed water bath-type aerobic composting system is used to carry out three sets of composting treatment of typical organic waste-household kitchen waste. In the 1st set of experiments solar energy is utilized, and solar energy is used in addition to electric energy supply for the 2nd set, while in the 3rd set no external energy is added. Study is conducted on degradation of organic matters and variations of enzyme activity during composting. The experimental results show that: 1) The results in the 1st set of experiments are greatly dependent on the outdoors weather, and compost varies with large fluctuation of stack temperature. The highest compost is only 43℃, belonging to the medium temperature compost; the highest stack temperature in the 2nd set is 56℃, and the high temperature composting period is 4d. The 3rd set of experiments is conducted in the completely normal temperature composting, reflecting the greater impact of external environmental temperature; 2) The final C/N value of the 2nd set of experiments is 10.2, lower than both 12.1 in the 1st set and 13.7 in the 3rd set, indicating that the material degradation is completed more thoroughly in the 2nd set of experiments; 3) In the process of the 2nd set of experiments, the activities of catalase, urease and protease are higher than those involving the 1st set and the 3rd set, indicating that the biochemical reaction in the 2nd set of experiments is more active. Among them, the roles played by catalase and urease are more active at the initial stage, and the temperature of the heap rapidly decreases and remains at a low level. The protease activity is more notable at the beginning and increases with the heap temperature growing higher, suggesting the difference in composting. It is found that the enzyme activitiy playing a leading role varies with the different stage. |
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| 备注/Memo: | 收稿日期: 2018－04－13. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省公益基金（LGF19E080006）; 宁波市科技局科研项目（2015C50043）. 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/第一作者: 徐超（1991－）, 男, 安徽阜阳人, 在读硕士研究生, 主要研究方向: 有机废物生物处理. E-mail: xuchao166@126.com *通信作者: 李兵（1977－）, 男, 江苏泰州人, 博士/副教授, 主要研究方向: 固体废物处理与资源化. E-mail: libing@nbu.edu.cn |
