荧光探针用于细胞内谷胱甘肽检测与成像
PDF下载 (52)张宇航,张 涛,吴祥武,喻盛容,吴永祥.荧光探针用于细胞内谷胱甘肽检测与成像[J].宁波大学学报(理工版),2025,38(5):96-104.DOI:10.20098/j.cnki.1001-5132.2025.0501
ZHANG Yuhang,ZHANG Tao,WU Xiangwu,YU Shengrong,WU Yongxiang.The fluorescent probe for the detection and imaging of intracellular glutathione[J].Journal of Ningbo University(Natural Science & Engineering Edition),2025,38(5):96-104.DOI:10.20098/j.cnki.1001-5132.2025.0501
| Title: | The fluorescent probe for the detection and imaging of intracellular glutathione |
| 作者: | 张宇航, 张 涛, 吴祥武, 喻盛容, 吴永祥 |
| Author(s): | ZHANG Yuhang, ZHANG Tao, WU Xiangwu, YU Shengrong, WU Yongxiang |
| 关键词: | 牛血清白蛋白金纳米簇; 树枝状大孔二氧化硅; 二氧化锰; 谷胱甘肽; 荧光纳米探针; 生物成像 |
| Keywords: | bovine serum albumin-gold nanoclusters; dendritic macroporous silica; manganese dioxide; glutathione; fluorescent nanoprobes; bioimaging |
| 分类号: | O657.3；TB383 |
| DOI: | 10.20098/j.cnki.1001-5132.2025.0501 |
| 文献标识码: | A |
| 摘要: | 聚焦于新型荧光纳米探针的构建，以牛血清白蛋白金纳米簇（BSA@AuNCs）作为荧光信号源，利用树枝状大孔二氧化硅（DSiO2）作为载体，将BSA@AuNCs负载于DSiO2孔洞内。在此基础上，通过精确调控高锰酸钾（KMnO4）质量浓度，在DSiO2表面原位生长出可降解的二氧化锰（MnO2）纳米片，最终成功制备出DSiO2-BSA@AuNCs@MnO2荧光纳米探针。结果表明：探针表面的MnO2可高效淬灭BSA@AuNCs荧光，且能与谷胱甘肽（GSH）特异性反应，促使BSA@AuNCs释放，恢复荧光信号，同时DSiO2-BSA@AuNCs@MnO2荧光纳米探针对GSH的检测展现出优秀特异性和高灵敏度，可有效地用于GSH检测。当KMnO4质量浓度为100.0 mg/L以及BSA@AuNCs荧光猝灭原强度89.30%时，并经GSH降解MnO2后，其恢复的荧光强度可达初始强度的81.34%。此外，细胞实验进一步验证了该荧光纳米探针能对细胞内GSH进行高效的生物成像，表明构建的DSiO2-BSA@AuNCs@MnO2荧光纳米探针可以作为细胞内GSH检测和生物成像的有效工具。 |
| Abstract: | This study focused on the development of novel fluorescent nanoprobes, for which bovine serum albumin-gold nanoclusters (BSA@AuNCs) were employed as the fluorescent signal source, with dendritic macroporous silica (DSiO2) served as the carrier. BSA@AuNCs were subsequently loaded into the pores of DSiO2. Thereafter, by precisely controlling the concentration of potassium permanganate (KMnO4), degradable manganese dioxide (MnO2) nanosheets were grown in situ on the surface of DSiO2, leading to the successful fabrication of DSiO2-BSA@AuNCs@MnO2 fluorescent nanoprobes. Experimental results demonstrate that the MnO2 nanosheets on the probe effectively quenched the fluorescence of BSA@AuNCs and specifically reacted with glutathione (GSH) to promote the release of BSA@AuNCs and the restoration of fluorescence signal. Moreover, the DSiO2-BSA@AuNCs@MnO2 fluorescent nanoprobe exhibits remarkable specificity and high sensitivity for GSH detection. When the concentration of KMnO4 was 100.0 mg/L, the fluorescence quenching efficiency of BSA@AuNCs reached 89.30%. Following degradation of MnO2 by GSH, the restored fluorescence intensity accounted for 81.34% of the initial intensity. Furthermore, cellular experiments confirmed the capability of the fluorescent nanoprobe in performing efficient intracellular GSH imaging, suggesting that the constructed DSiO2-BSA@AuNCs@MnO2 fluorescent nanoprobe is a promising tool for intracellular GSH detection and biological imaging. |
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| 备注/Memo: | 收稿日期：2025−05−01 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ 基金项目：浙江省自然科学基金（LY23B050002） 第一作者：张宇航，硕士研究生，主要研究方向为化学与生物传感。E-mail: 18858243281@163.com *通信作者：吴永祥，副教授，主要研究方向为化学与生物传感。E-mail: wuyongxiang@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
