微波消解-塞曼石墨炉原子吸收光谱法检测贝类中的铬
PDF下载 (462)顾 捷,刘 琴,祝 银,王范盛,方旭波*.微波消解-塞曼石墨炉原子吸收光谱法检测贝类中的铬[J].宁波大学学报(理工版),2013,27(01):76-79.DOI:
GU Jie,LIU Qin,ZHU Yin,WANG Fan-sheng,FANG Xu-bo*.Determination of Chromium in Shellfish Using Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometer with Zeeman Correction[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,27(01):76-79.DOI:
| Title: | Determination of Chromium in Shellfish Using Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometer with Zeeman Correction |
| 作者: | 顾 捷, 刘 琴, 祝 银, 王范盛, 方旭波* |
| Author(s): | GU Jie, LIU Qin, ZHU Yin, WANG Fan-sheng, FANG Xu-bo* |
| 关键词: | 微波消解; 塞曼石墨炉原子吸收光谱法; 贝类; 铬 |
| Keywords: | microwave digestion; graphite furnace atomic absorption spectrometer(GF-AAS) with zeeman correction; shellfish; chromium |
| 分类号: | O657.31 |
| 文献标识码: | A |
| 摘要: | 建立了分析贝类样品中铬含量的微波消解-塞曼石墨炉原子吸收光谱法, 并对样品前处理方法和检测条件进行探讨. 实验结果表明, 利用微波消解进行前处理的样品溶样均匀, 样品消解完全. 石墨炉铬工作曲线浓度范围设定在0.00~15.00 μg·L-1之间时, 铬浓度与其吸光值呈良好的曲线关系, 得出的新合理一元二次方程相关系数r=1.0000. 同时对3种贝类样品进行平行测定和加标回收率测定, 平行性好, 加标回收率保持在82%~95%. 并利用国家标准物质海带和贻贝成份分析标准物质进行质量控制, 得到的测定值在标准证书的允许误差范围内, 相对标准偏差分别为2.93%和2.54%. 表明该方法具有操作简便、灵敏度高、准确可靠等优点, 适合贝类等水产品中铬含量的检测. |
| Abstract: | In this paper, the method for determining chromium in shellfish is studied using Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometry (GF-AAS) with Zeeman correction. The results of analysis demonstrate that by Microwave Digestion, the samples are digested evenly and completely. When chromium working curve is linear within the range of 0.00~15.00μg?L-1, it is found that chromium concentration can be described by a smooth curve with the correlation coefficient r=1.0000. Based on this working curve, the results of Sinonovacula constricta can be determined. Comparing Tegillarca granosa with Mussel in their recovery rates, it is found that the latter can reach as high as 82%~95%. Moreover, the accuracy and precision of this method are also tested with the national standard substance (Laminaria japonica Aresch and Mussel). It shows that the errors of the results fall within the permissible range. The relative standard deviations were 2.93% and 2.54%. To conclude, with high sensibility, high quality and simple procedure, the Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometry (GF-AAS) equipped with Zeeman correction, is suitable for determination of chromium in shellfish samples． |
| 参考文献 /References: | [1]. 铬污染及其生态效应[J]. 环境科学与技术, 2002, 25(增):8-9, 28. [2].GB/T 5009.123-2003, 食品中铬的测定[S]. [3].王海涛, 蔺玉华, 卢玲, 等.[J]. 光谱实验室, 2003, 20(6):955-958. [4].许萍, 牟仁祥, 曹赵云, 等. 密闭式微波消解方法综述[J]. 光谱实验室, 2009, 26(1): 57-59. [5].吕海燕, 曾江宁, 周青松, 等. 浙江沿岸贝类生物体中Hg、Cd、Pb、As含量的分析[J]. 东海海洋, 2001, 19(3): 25-31. [6].励建荣, 李学鹏, 王丽, 等. 贝类对重金属的吸收转运与累积规律研究进展[J]. 水产科学, 2007, 26(1):51-54. [7].兰柳春, 廖永志, 黎小正, 等. 微波消解石墨炉原子吸收法测定水产品中铅的研究[J]. 广西农业科学, 2009, 40(6):764-767. |
| 备注/Memo: | 收稿日期: 2013?07?29. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 浙江省科技计划资助项目（2012F20026）.第一作者: 顾 捷（1980－）, 女, 浙江舟山人, 助理工程师, 主要研究方向: 渔业环境与水产品质量安全. E-mail: 2210346257@qq.com*通信作者: 方旭波（1972－）, 男, 浙江舟山人, 副教授, 主要研究方向: 食品科学与工程、食品质量与安全. E-mail: fxb70@163.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
