响应面优化草鱼发酵工艺条件
PDF下载 (582)周吉,裘迪红 *.响应面优化草鱼发酵工艺条件[J].宁波大学学报(理工版),2014,27(02):13-18.DOI:
ZHOU Ji,QIU Di-hong *.Optimization on Fermenting Conditions of Grass Crap with Response Surface Method[J].Journal of Ningbo University(Natural Science & Engineering Edition),2014,27(02):13-18.DOI:
| Title: | Optimization on Fermenting Conditions of Grass Crap with Response Surface Method |
| 作者: | 周吉, 裘迪红 * |
| Author(s): | ZHOU Ji, QIU Di-hong * |
| 关键词: | 草鱼; 发酵工艺; 植物乳杆菌; 响应面法 |
| Keywords: | grass crap; fermentation; Lactobacillus plantarum; response surface methodology |
| 分类号: | TS254.4 |
| 文献标识码: | A |
| 摘要: | 利用一株植物乳杆菌为发酵菌种, 对草鱼进行发酵. 以总酸含量为主要指标, 在单因素试验的基础上, 运用Design-Expert7 Trial统计分析软件, 设计4因素5水平试验, 建立加盐量、发酵温度、发酵时间和接种量的二次回归模型, 得到草鱼发酵的最优条件. 研究结果表明: 最优的发酵工艺条件为加盐量5.5％, 发酵温度29.5℃, 发酵时间25.9h, 接种量7.4％, 在此条件下发酵草鱼的总酸含量可达15.61‰, 且感官评分值达到82.6分. |
| Abstract: | In order to effectively prolong the preserving period of the processed grass crap and improve its flavor and commercial value, the experiment conducted in this research tries to ferment grass crap by applying Lactobacillus plantarum as the strains. Taking the total acid content as the main variable, and based on a single-factor experiment, a four-factor and five-level test in this study is designed using the Design-Expert 7 statistical analysis software, and the optimal condition of the fermentation of grass crap is obtained through a quadratic regression model which contains the variables of the amount of salt, fermentation temperature, fermentation time and inoculation amount. Based on the test result, the optimal fermentation condition is that the amount of salt, the fermentation temperature, the fermentation time and the inoculation amount should be configured with ratio of 5.5％, 29.5℃, 25.9h and 7.4％ respectively, and only on this condition can the total acid content and the score of sensory assessment reach 15.61‰ and 82.6, respectively. |
| 参考文献 /References: | [1].余兰玉. 鱼类保鲜加工技术[J]渔业致富指南, 2006 (13):54-56. [2].曾令彬. 腊鱼加工中微生物菌群、理化特性及挥发性成分的研究[D]. : 华中农业大学, 2008. [3].李气清. 我国肉类食品的发展状况与质量监控[J]. 肉类研究, 2001(1):729-731. [4].张燕, 周常义, 苏文金, 等. 乳酸菌及其代谢产物在食品保鲜中的应用研究进展[J]. 农产品加工学刊, 2012. [5].Manuela F. Accelerated ripening of dry fermented sausages[J]. Trends in Food Science and Technology, 2000, 6(11):202-206. [6].谭汝成, 欧阳加敏, 卢晓莉, 等. 接种植物乳杆菌和戊糖片球菌发酵对鱼[J]. 食品科学, 2007, 12. [7].Wonnop V, Soottawat B, Hitapha T S. Changes in microbiological, biochemical and physico-chemical properties of ham inoculated with different inoculum levels of Lactobacillus curvatus[J]. Swiss Society of Food Science and Technology, 2006(39):814-826. [8].李宗军, 江汉湖, 李红琼, 等. 湘西侗族传统发酵肉的产品特性[J]. 湖南农业大学学报, 2002, 28(1):61-63. [9].周传云, 聂明, 万佳蓉. 发酵肉制品的研究进展[J]. 食品与机械, 2004, 20(2):27-30. [10].曹荣, 刘淇, 殷邦忠. 响应面法优化海参性腺酶解工艺[J]. 食品科学, 2012, 2(33):29-33. [11].姜松, 车长远, 栗春艳. 大豆分离蛋白凝胶制备条件的响应曲面法优化研究[J]. 中国粮油学报, 2009, 24(2): 46-49. [12].冯金晓, 薛长湖, 徐莹, 等. 响应面法优化Protease M 酶解牡蛎工艺的研究[J]. 食品科技, 2009, 34(5):184- 187. [13].曹文红, 章超桦, 洪鹏志. 响应面法优化南美白对虾虾头自溶工艺的研究[J]. 中国食品学报, 2009, 9(1):158- 162. [14].王友湘, 陈庆森, 阎亚丽. 用于乳酸菌分离鉴定的几种培养基的筛选及应用[J]. 食品科学, 2007(9):374-378. [15].于修. 乳酸菌高密度培养及浓缩型发酵剂研究[D]. 南京: 南京工业大学, 2004. [16].赵德畅, 宁喜斌. 短乳杆菌发酵鲢鱼块工艺参数的优化研究[J]. 黑龙江农业科学, 2010(11):103-105. [17].龚吉军, 唐静, 李忠海, 等. 响应曲面法优化[J]. 中国食品学报, 2010, 4(10):219-226. [18].张水华. 食品分析[M]. 北京: 中国轻工业出版社, 2007. [19].Berdague J L, Monteil P, Montel M C. Effects of starter cultures on the formation of flavour compounds in dry sausage[J]. Meat Science, 1993, 3(35):275-287. [20].Guo Xingfeng, Tian Shaojun, Small D M. Generation of meat-like flavourings from enzymatic hydrolysates of proteins from Brassica sp.[J]. Food Chemistry, 2010, 119(1):167-172. |
| 备注/Memo: | 收稿日期: 2013-08-28. 宁波大学学报（理工版）网址http://journallg.nbu.edu.cn/ 基金项目: 浙江省高校人才工程项目（2010442）. 第一作者: 周 吉（1988－）, 女, 江苏淮安人, 在读硕士研究生, 主要研究方向: 水产品加工与保鲜. E-mail: 877036332@qq.com *通信作者: 裘迪红（1966－）, 女, 浙江舟山人, 副教授, 主要研究方向: 水产品安全. E-mail: qiudihong@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
