固相微萃取-气相色谱串联质谱法测定姜黄素中残留溶剂
PDF下载 (18933)李 艳,张宇航,王 洁,张金荣 *,严小军.固相微萃取-气相色谱串联质谱法测定姜黄素中残留溶剂[J].宁波大学学报(理工版),2017,30(5):30-36.DOI:
LI Yan,ZHANG Yu-hang,WANG Jie,ZHANG Jin-rong *,YAN Xiao-jun.Determination of residual solvents in curcumin by solid phase microextraction coupled with gas chromatography tandem mass spectrometry[J].Journal of Ningbo University(Natural Science & Engineering Edition),2017,30(5):30-36.DOI:
| Title: | Determination of residual solvents in curcumin by solid phase microextraction coupled with gas chromatography tandem mass spectrometry |
| 作者: | 李 艳, 张宇航, 王 洁, 张金荣 *, 严小军 |
| Author(s): | LI Yan, ZHANG Yu-hang, WANG Jie, ZHANG Jin-rong *, YAN Xiao-jun |
| 关键词: | 固相微萃取; 气相色谱串联质谱; 姜黄素; 残留溶剂 |
| Keywords: | solid phase microextraction; gas chromatography tandem mass spectrometry; curcumin; residual solvents |
| 分类号: | O658 |
| 文献标识码: | A |
| 摘要: | 采用固相微萃取-气相色谱质谱联用分析方法, 对姜黄素粉末中溶剂残留情况进行定量分析. 在优化实验条件下, 建立了甲醛、甲醇、乙醇等21种生产过程中常用溶剂的标准曲线, 相关系数均大于0.9990, 检测限0.001~0.007?g?g-1, 定量限0.003~0.022?g?g-1. 高、中、低浓度下的加标回收率范围为81.56%~105.54%. 结果表明, 该方法线性关系良好, 具有较高的回收率和精密度, 相对于传统方法分析目标更全面、应用范围更广. 采用该方法对姜黄素样品溶剂残留进行定量分析, 其结果对姜黄素的生产、制备过程的质量控制具有指导意义. |
| Abstract: | A solid phase microextraction coupled with gas chromatography tandem mass spectrometry method was established for the determination of residual solvents in curcumin. Under the optimized experimental conditions, the correlation coefficients of 21 solvents of formaldehyde, methanol, ethanol, etc are more than 0.9990, limits of detection are in the range of 0.001-0.007?g?g-1, limits of quantitation are in the range of 0.003-0.022?g?g-1. Spiked recoveries of target solvents in high, intermediate and low level are in the range of 81.56%-105.54%. The results indicate that this method has excellent linear relationships, high recovery and good precision. It covers more analysis targets and wider application range than traditional methods. It is suitable for the quantitative analysis of residual solvents in curcumin, and it is instructional for the quality control of the extraction and production of curcumin. |
| 参考文献 /References: | [1] MAITI M, CHATTOPADHYAY K, VERMA M, et al. Curcumin protects against nicotine-induced stress during protein malnutrition in female rat through immunomodu- lation with cellular amelioration[J]. Molecular Biology Reports, 2015, 42(12):1623-1637. [2] LIU W, YUAN J C, ZHU H T, et al. Curcumin reduces brain-infiltrating T lymphocytes after intracerebral hemorrhage in mice[J]. Neuroscience Letters, 2016, 620:74-82. [3] KANG D, LI B, LUO L, et al. Curcumin shows excellent therapeutic effect on psoriasis in mouse model[J]. Biochimie, 2016, 123:73-80. [4] KUMAR A, KAUR G, KANSAL S K, et al. Enhanced solubilization of curcumin in mixed surfactant vesicles[J]. Food Chemistry, 2016, 199:660-666. [5] ZHANG W, CHEN C, SHI H, et al. Curcumin is a biologically active copper chelator with antitumor activity [J]. Phytomedicine, 2016, 23(1):1-8. [6] SARIKA P R, NIRMALA R J. Curcumin loaded gum arabic aldehyde-gelatin nanogels for breast cancer therapy[J]. Materials Science & Engineering C Materials for Biological Applications, 2016, 65:331-337. [7] ZHENG Z L, SUN Y H, LIU Z L, et al. The effect of curcumin and its nanoformulation on adjuvant-induced arthritis in rats[J]. Drug Design, Development and Therapy, 2015, 9:4931-4942. [8] SAHEBKAR A. A systematic review and meta-analysis of randomized controlled trials investigating the effects of curcumin on blood lipid levels[J]. Clinical Nutrition, 2014, 33(3):406-414. [9] KWON Y. Association of curry consumption with blood lipids and glucose levels[J]. Nutrition Research and Practice, 2016, 10(2):212-220. [10] GUNNINK L K, ALABI O D, KUIPER B D, et al. Curcumin directly inhibits the transport activity of GLUT1[J]. Biochimie, 2016, 125:179-185. [11] JEENGER M K, SHRIVASTAVA S, YERRA V G, et al. Curcumin: A pleiotropic phytonutrient in diabetic complications[J]. Nutrition, 2015, 31(2):276-282. [12] RAMAN T, RAMAR M, ARUMUGAM M, et al. Cytoprotective mechanism of action of curcumin against cataract[J]. Pharmacological Reports, 2016, 68(3):561- 569. [13] HASHEM R M, MOHAMED R H, DINA M A E M. Effect of curcumin on TNFR2 and TRAF2 in unilateral ureteral obstruction in rats[J]. Nutrition, 2016, 32(4):478- 485. [14] NABIUNI M, MOHAMMADI S, KAYEDPOOR P, et al. The effect of curcumin on the estradiol valerate-induced polycystic ovary in rats[J]. Feyz Journal of Kashan University of Medical Sciences, 2015, 18(6):515-523. [15] SALAHSHOOR M, MOHAMADIAN S, KAKABARAEI S, et al. Curcumin improves liver damage in male mice exposed to nicotine[J]. African Journal of Traditional Complementary and Alternative Medicines, 2016, 6(2): 176-183. [16] ANJUM S, GUPTA A, SHARMA D, et al. Development of novel wound care systems based on nanosilver nanohydrogels of polymethacrylic acid with Aloe vera and curcumin[J]. Materials Science & Engineering C Materials for Biological Applications, 2016, 64:157-166. [17] CARLA M T, MILAGROS M, MAR?A M D Z, et al. Multiple responses optimization in the development of a headspace gas chromatography method for the determination of residual solvents in pharmaceuticals[J]. Journal of Pharmaceutical and Biomedical Analysis, 2015, 5(5):296-306. [18] GRODOWSKA K, PARCZEWSKI A. Analytical methods for residual solvents determination in pharmaceutical products[J]. Acta Poloniae Pharmaceutica, 2010, 67(1): 13-26. [19] KILIAN K, SZKUTNIK A P K W, PYRZYN K. A fast method for the determination of residual solvents in 18FDG and 11C-methionine samples[J]. Microchemical Journal, 2014, 115(115):95-99. [20] KE Y Y, LI W J, WANG Y, et al. Comparison of fully-automated headspace single drop microextraction and headspace solid phase microextraction techniques for rapid analysis of No. 6 solvent residues in edible oil[J]. Microchemical Journal, 2014, 117(21):187-193. [21] 徐清, 秦国富, 邹柯婷, 等. 顶空气相色谱法测定食用油中溶剂残留的研究[J]. 食品研究与开发, 2015, 36 (23):138-141. [22] 杨丽蓉, 赵红磊, 张筱. 顶空气相色谱法同时测定原料药中多种残留溶剂的应用研究[J]. 中国执业药师, 2016, 13(9):10-12. |
| 备注/Memo: | 收稿日期: 2016－11－07. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省重大科技专项（2012C12907-6）; 宁波市工业重大专项（2014B11005）; 宁波市创新团队项目（2011B81007）. 第一作者: 李艳（1980－）, 女, 山西忻州人, 助理实验师, 主要研究方向: 生物化学. E-mail: liyan1@nbu.edu.cn *通信作者: 张金荣（1975－）, 女, 吉林敦化人, 讲师, 主要研究方向: 天然产物化学. E-mail: zhangjinrong@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
