饥饿再投喂对黑鱾幼鱼消化酶活性与血液生理生化指标的影响
PDF下载 (215)张怡波,吕慧明,袁 子,徐善良,王丹丽.饥饿再投喂对黑鱾幼鱼消化酶活性与血液生理生化指标的影响[J].宁波大学学报(理工版),2019,32(5):12-16.DOI:
ZHANG Yibo,L? Huiming,YUAN Zi,XU Shanliang,WANG Danli.Effects of starvation and refeeding on digestive enzyme activities and blood indices in juvenile Girella leoninas[J].Journal of Ningbo University(Natural Science & Engineering Edition),2019,32(5):12-16.DOI:
| Title: | Effects of starvation and refeeding on digestive enzyme activities and blood indices in juvenile Girella leoninas |
| 作者: | 张怡波, 吕慧明, 袁 子, 徐善良, 王丹丽 |
| Author(s): | ZHANG Yibo, L? Huiming, YUAN Zi, XU Shanliang, WANG Danli |
| 关键词: | 黑鱾; 饥饿; 再投喂; 消化酶活性; 血液生理生化指标 |
| Keywords: | Girella leonina; starvation; refeeding; digestive enzyme activity; blood indices |
| 分类号: | S917.4 |
| 文献标识码: | A |
| 摘要: | 为研究饥饿再投喂对黑鱾幼鱼消化酶活性和血液生理生化指标的影响, 设置3个饥饿实验组(S5、S10、S15)及对照组(C组), 各实验组饥饿后均恢复投喂5d. 结果显示: (1)饥饿胁迫下, 消化酶活性总体呈下降趋势; 饥饿对淀粉酶活性影响不显著. (2)复投喂5d后, 各饥饿组胃中蛋白酶、脂肪酶活性相对稳定或继续下降; 肝脏和肠中蛋白酶S10和S15组迅速回升, 脂肪酶活性也呈恢复趋势. (3)各饥饿组白细胞、红细胞、血红蛋白等含量显著下降; 复投喂后, 白细胞及S5、S10组的红细胞和血红蛋白含量回升至对照组水平. (4)血糖在饥饿5d时显著下降, 之后基本稳定; 总胆固醇和甘油三酯随饥饿时间延长而升高; 总蛋白含量先降后升, 白蛋白球蛋白比下降; 复投喂后血糖、白蛋白球蛋白比回升. 分析认为, 黑鱾幼鱼在短期饥饿中, 前期主要消耗脂肪, 后期动用蛋白质作为维持基础代谢的能量. |
| Abstract: | The current study was aimed to investigate the effects of starvation and refeeding on digestive enzyme activity, blood physiological and biochemical indexes of juvenile black bream. Three experimental groups of starvation (S5, S10, S15) and a control group (Group C) were included. All the experimental groups were refed for 5d after starvation. The results showed that digestive enzymes activity exhibited a downward trend under the stress of starvation. Starvation had little effect on amylase activity. Furthermore, the activity of pepsin and lipase in each starvation group achieved relative stability or experienced a continued decline after refeeding for 5d. Protease activity of liver and intestine of S10, S15 groups and lipase activity took on a recovery trend. In addition, the content of white blood cells, erythrocytes and hemoglobin decreased significantly in all starvation groups. Following refeeding, the white blood cell count and the erythrocytes and hemoglobin content in S5, S10 groups returned to the control group level. The blood-sugar concentration decreased after starvation for 5d, and then was relatively stable. Total cholesterol and triglyceride increased time-dependently. The total protein content declined first and subsequently increased. The albumin-globulin ratio decreased. After refeeding, the blood-sugar concentration and albumin- globulin ratio increased. Analysis suggested that during short-term starvation, juvenile black bream mainly consumed lipid in the early stage and began to use protein as energy to maintain basic metabolism during the later stage. |
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| 备注/Memo: | 收稿日期: 2018－12－12. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（31872586）; 浙江省公益技术应用研究（2017C32015）. 第一作者: 张怡波（1992－）, 女, 河南周口人, 在读硕士研究生, 主要研究方向: 鱼类生态学. E-mail: zyb15058456761@163.com *通信作者: 徐善良（1962－）, 男, 浙江舟山人, 教授, 主要研究方向: 海水鱼类健康养殖. E-mail: xushanliang@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
