金枪鱼加工副产物酶解液对顶头孢霉菌发酵产物的影响
PDF下载 (356)王 欢,李 晔,戴 娟,苏秀榕.金枪鱼加工副产物酶解液对顶头孢霉菌发酵产物的影响[J].宁波大学学报(理工版),2019,32(5):17-22.DOI:
WANG Huan,LI Ye,DAI Juan,SU Xiurong.Effects of hydrolysates of tuna processing by-products on the fermentation products of Cephalosporium acremonium[J].Journal of Ningbo University(Natural Science & Engineering Edition),2019,32(5):17-22.DOI:
| Title: | Effects of hydrolysates of tuna processing by-products on the fermentation products of Cephalosporium acremonium |
| 作者: | 王 欢, 李 晔, 戴 娟, 苏秀榕 |
| Author(s): | WANG Huan, LI Ye, DAI Juan, SU Xiurong |
| 关键词: | 金枪鱼副产物; 酶解液; 顶头孢霉菌; 头孢菌素C |
| Keywords: | tuna by-products; hydrolysates; Cephalosporium acremonium; cephalosporin C |
| 分类号: | TS254.9 |
| 文献标识码: | A |
| 摘要: | 以金枪鱼加工副产物为原料, 研究其酶解液对顶头孢霉菌(Cephalosporium acremonium)发酵产物的影响. 以头孢菌素C(CPC)的产量为指标, 通过正交实验和响应面方法, 研究不同添加量的酶解液对顶头孢霉菌发酵产抗生素的作用, 再利用气相-质谱联用仪(GC-MS)分析酶解液对顶头孢霉菌代谢物的影响. 研究结果显示: 在顶头孢霉菌的发酵培养基中, 酶解液的添加量为30.86mL<span style="font-family: "Times New Roman"; font-size: 10pt;">×L-1时, CPC产量最高, 为8.106g<span style="font-family: "Times New Roman"; font-size: 10pt;">×L-1, 比未添加酶解液增加67.82％; 酶解液的加入显著改变了顶头孢霉菌代谢物的种类和含量. |
| Abstract: | The by-products of tuna were used as raw materials to study the effects of the hydrolysates on the fermentation products of Cephalosporium acremonium. Firstly, based on the yield of cephalosporin C (CPC), the effects of different amounts of hydrolysates on the production of antibiotics by C. acremonium were studied by orthogonal experiment and response surface method. The results showed that in the fermentation medium with the amount of hydrolysates being 30.86 mL<span style="font-family: "Times New Roman"; font-size: 10pt;">×L-1, the production of CPC reached a maximum of 8.106 g<span style="font-family: "Times New Roman"; font-size: 10pt;">×L-1. It was 67.82％ higher than that without the hydrolysates. Further, the effect of the hydrolysates on the metabolites of C. acremonium was qualitatively and quantitatively analyzed by GC-MS. It was found that the addition of the hydrolysates significantly changed the species and content of the metabolites of C. acremonium. It was of great significance to improve the production of CPC and the comprehensive economic and environmental benefits of tuna processing that uses the resources of tuna processing by-products to develop fermentation industrial medium. |
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| 备注/Memo: | 收稿日期: 2018－11－27. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市海洋经济创新发展区域示范项目（2013716, 20161216）. 第一作者: 王欢（1994－）, 女, 河北石家庄人, 在读硕士研究生, 主要研究方向: 食品工程. E-mail: 1583301194@qq.com *通信作者: 李晔（1980－）, 女, 浙江宁波人, 副教授, 主要研究方向: 食品安全、生物与分子生物学. E-mail: liye@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
