吡唑醚菌酯和嘧菌酯在铁皮石斛中的残留及其对黄酮含量的影响
PDF下载 (227)雷 圆,张昌朋,孙凤婷,叶 会,陆兰菲,赵学平,曾小群.吡唑醚菌酯和嘧菌酯在铁皮石斛中的残留及其对黄酮含量的影响[J].宁波大学学报(理工版),2023,36(4):97-103.DOI:10.20098/j.cnki.1001-5132.2023.0236
LEI Yuan,ZHANG Changpeng,SUN Fengting,YE Hui,LU Lanfei,ZHAO Xueping,ZENG Xiaoqun.Residues of pyraclostrobin and azoxystrobin in Dendrobium officinale Kimura et Migo and their effects on flavonoid contents[J].Journal of Ningbo University(Natural Science & Engineering Edition),2023,36(4):97-103.DOI:10.20098/j.cnki.1001-5132.2023.0236
| Title: | Residues of pyraclostrobin and azoxystrobin in Dendrobium officinale Kimura et Migo and their effects on flavonoid contents |
| 作者: | 雷 圆, 张昌朋, 孙凤婷, 叶 会, 陆兰菲, 赵学平, 曾小群 |
| Author(s): | LEI Yuan, ZHANG Changpeng, SUN Fengting, YE Hui, LU Lanfei, ZHAO Xueping, ZENG Xiaoqun |
| 关键词: | 吡唑醚菌酯; 嘧菌酯; 铁皮石斛; 农药残留; 黄酮 |
| Keywords: | pyraclostrobine; azoxystrobin; Dendrobium officinale Kimura et Migo; residue; flavonoid contents |
| 分类号: | S481+.8; S567.23 |
| DOI: | 10.20098/j.cnki.1001-5132.2023.0236 |
| 文献标识码: | A |
| 摘要: | 为了解吡唑醚菌酯和嘧菌酯在铁皮石斛中的残留动态及吡唑醚菌酯和嘧菌酯对铁皮石斛中黄酮含量的影响, 通过盆栽实验, 设置了吡唑醚菌酯推荐低剂量(T1)、吡唑醚菌酯推荐高剂量(T2)、嘧菌酯推荐低剂量(T3)、嘧菌酯推荐高剂量(T4)和空白对照(CK)5个处理, 采用QuEChERS-液相色谱-串联质谱法和紫外分光光度计检测法, 测定铁皮石斛中吡唑醚菌酯和嘧菌酯的残留量和黄酮含量. 结果表明: (1)建立的方法准确度及灵敏度良好, 平均回收率为88.85％~117.50％, 相对标准偏差为1.75％~13.92％, 满足铁皮石斛中吡唑醚菌酯和嘧菌酯残留检测要求. (2)通过盆栽实验发现, 吡唑醚菌酯和嘧菌酯在铁皮石斛中消解速率较慢, 半衰期分别为110.45~145.45d和68.42~170.39d. 90d时, T1和T2处理组在铁皮石斛中消解率和残留量分别为45.65％、5.23mg?kg-1和40.70％、7.65mg?kg-1; T3和T4处理组在铁皮石斛中消解率和残留量分别为61.23％、2.51mg?kg-1和36.56％、5.50mg?kg-1. (3)通过对90d铁皮石斛样品中黄酮含量的检测发现, 吡唑醚菌酯和嘧菌酯处理在短时间内可显著提高铁皮石斛中黄酮含量, 随着时间延长黄酮含量趋于稳定. |
| Abstract: | The present study was designed to study the residual dynamics of pyraclostrobin and azoxystrobin in Dendrobium officinale Kimura et Migo. In order to determine the effects of pyraclostrobin and azoxystrobin on flavonoid contents in this plant, five treatments were set up: pyraclostrobin recommended low dose (T1), pyraclostrobin recommended high dose (T2), azoxystrobin recommended low dose (T3), azoxystrobin recommended high dose (T4), and control check (CK). QuEChERS-liquid chromatography-mass spectrometry and ultraviolet spectrophotometry were used to detect pyraclostrobin and azoxystrobin residues, as well as flavonoid contents. The results showed that the average recoveries (88.85％-117.50％) and relative standard deviations (1.75％-13.92％) of pyraclostrobin and azoxystrobin in Dendrobium officinale Kimura et Migo were in line with residual requirements, within the range of spiked levels. Furthermore, potted experiment showed that pyraclostrobin and azoxystrobin had a slower digestion rate in Dendrobium officinale Kimura et Migo, with half-lives ranging from 110.45-145.45 d and 68.42-170.39 d respectively. At 90 d, the digestion rates and residues in the recommended low dose (T1) and high dose (T2) of pyraclostrobin treatment groups were 45.65％, 5.23 mg?kg-1and 40.70％, 7.65 mg?g-1 respectively. The digestion rates and residues in the recommended low dose (T3) and high dose (T4) of azoxystrobin treatment groups were 61.23％, 2.51 mg?kg-1, and 36.56％, 5.50 mg?kg-1 respectively. Through the detection of flavonoid contents in 90 d Dendrobium officinale Kimura et Migo samples, pyraclostrobin and azoxystrobin significantly increased the flavonoid contents in a short period. In contrast, the flavonoid contents appeared to be stable with the extension of time. |
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| 备注/Memo: | 收稿日期: 2023－02－22. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 国家自然科学基金（32072195）.第一作者: 雷圆（1999－）, 女, 河南信阳人, 在读硕士研究生, 主要研究方向: 食品安全. E-mail: leiyuan76541@163.com*通信作者: 曾小群（1982－）, 女, 四川仁寿人, 教授, 主要研究方向: 食品安全等. E-mail: zxqun3447@126.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
