高效液相色谱-串联质谱法测定果汁中恩镰孢菌素和白僵菌素
PDF下载 (251)邓 涛,吉小凤,肖英平,汪 雯,吕文涛,王小骊,吴 振,杨 华.高效液相色谱-串联质谱法测定果汁中恩镰孢菌素和白僵菌素[J].宁波大学学报(理工版),2023,36(6):50-56.DOI:10.20098/j.cnki.1001-5132.2022.1006
DENG Tao,JI Xiaofeng,XIAO Yingping,WANG Wen,L? Wentao,WANG Xiaoli,WU Zhen,YANG Hua.Determination of enniatins and beauvericin in fruit juice by high performance liquid chromatography-tandem mass spectrometry[J].Journal of Ningbo University(Natural Science & Engineering Edition),2023,36(6):50-56.DOI:10.20098/j.cnki.1001-5132.2022.1006
| Title: | Determination of enniatins and beauvericin in fruit juice by high performance liquid chromatography-tandem mass spectrometry |
| 作者: | 邓 涛, 吉小凤, 肖英平, 汪 雯, 吕文涛, 王小骊, 吴 振, 杨 华 |
| Author(s): | DENG Tao, JI Xiaofeng, XIAO Yingping, WANG Wen, L? Wentao, WANG Xiaoli, WU Zhen, YANG Hua |
| 关键词: | 果汁; 恩镰孢菌素; 白僵菌素; 食品安全 |
| Keywords: | fruit juice; enniatins; beauvericin; food safety |
| 分类号: | O675.63; TS201.6 |
| DOI: | 10.20098/j.cnki.1001-5132.2022.1006 |
| 文献标识码: | A |
| 摘要: | 建立高效液相色谱-串联质谱法测定果汁中恩镰孢菌素和白僵菌素检测方法. 样品用V(乙腈)V(水)V(甲酸)混合液(79201)提取, QuEChERS填料包(无水MgSO4、0无水CH3COONa和 NaCl)盐析, 100mg C18净化, 经过液相色谱柱分离, 2mmol·L–1乙酸铵水溶液-甲醇为流动相梯度洗脱, 由ESI源扫描, 多反应监测模式, 基质外标法定量分析. 该方法中, 恩镰孢菌素A(ENA)、恩镰孢菌素B(ENB)和白僵菌素(BEA)在0.1~200μg·L–1以及恩镰孢菌素A1(ENA1)和恩镰孢菌素B1(ENB1)在1~200μg·L–1范围内线性关系良好, 且线性相关系数(R2)均大于0.9989, 相对标准偏差范围为1.7%~11.7%. 3个不同加标浓度的添加回收率为88.0%~114.1%, 样品检测限为1.5?g·kg–1, 样品定量限为5?g·kg–1. 本文建立的方法简单、高效、准确, 满足果汁中恩镰孢菌素和白僵菌素污染筛查需求. |
| Abstract: | A high performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of enniatins and beauvericin in fruit juices. Samples were extracted with V(acetonitrile)V(water)V(formic acid) mixture solution (79201), salted out extraction by QuEChERS packed packages ( anhydrous MgSO4, anhydrous CH3COONa and NaCl), purified by 100mg C18, and separated by liquid chromatography column with 2mmol·L–1 ammonium acetate aqueous solution-methanol as mobile phase gradient elution. ESI positive ion mode scanning, multiple response monitoring mode detection, and matrix external standard method quantitative analysis were employed. The linearity was in the range of 0.1-200μg·L–1 for ENA、ENB and BEA, and the linearity was in the range of 1-200μg·L–1 for ENA1 and ENB1, with the correlation coefficients (R2) greater than 0.9989 and relative standard deviations ranging from 1.7% to 11.7%. The recoveries were ranged from 88.0% to 114.1%. The samples’ limits of detection were 1.5μg·kg–1, and their limits of quantitation were 5μg·kg–1. The method established in this study is demonstrated as being simple, efficient, and accurate, which can satisfy the requirements of screening tests for enniatins and beauvericin in fruit juice samples. |
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| 备注/Memo: | 收稿日期:2022-10-12.宁波大学学报（理工版）网址:http://journallg.nbu.edu.cn/ 基金项目:科技部国家重点研发计划政府间合作项目（2019YFE0103900）. 第一作者:邓涛（1997－）,男,安徽滁州人,在读硕士研究生,主要研究方向:食品安全与营养.E-mail:dengtao8822@163.com *通信作者:吴振（1985－）,男,河南新乡人,博士/副教授,主要研究方向:食品加工与安全.E-mail:wuzhen@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
