4种重金属离子对海水青鳉胚胎发育及仔鱼的急性毒性研究
PDF下载 (291)杨志艳,薛雅芳,徐永健.4种重金属离子对海水青鳉胚胎发育及仔鱼的急性毒性研究[J].宁波大学学报(理工版),2021,34(5):9-15.DOI:
YANG Zhiyan,XUE Yafang,XU Yongjian.Effects of four heavy metal ions on embryonic development and larval growth of Oryzias melastigma[J].Journal of Ningbo University(Natural Science & Engineering Edition),2021,34(5):9-15.DOI:
| Title: | Effects of four heavy metal ions on embryonic development and larval growth of Oryzias melastigma |
| 作者: | 杨志艳, 薛雅芳, 徐永健 |
| Author(s): | YANG Zhiyan, XUE Yafang, XU Yongjian |
| 关键词: | 海水青鳉; 胚胎发育; 仔鱼; 胚胎; 重金属; 急性毒性 |
| Keywords: | Oryzias melastigma; embryonic development; larval; embryo; heavy metals; acute toxicity |
| 分类号: | S917.4 |
| 文献标识码: | A |
| 摘要: | 为研究重金属对海洋生物的毒性影响及敏感性, 本文以海水模式鱼—–海水青鳉(Oryzias melastigma)为对象, 分析4种代表性海水重金属污染物铜、镉、铅、汞对其胚胎发育和仔鱼生长期的24h急性毒性. 结果显示: 海水青鳉发育阶段对4种重金属离子都很敏感, 且都产生明显毒性影响. Hg2+、Cu2+、Pb2+、Cd2+对海水青鳉胚胎发育期24h LC50最低值分别为2.34μg?L-1 (I期)、0.72mg?L-1 (IV期)、22.65μg?L-1 (I期)、27.32μg?L-1 (I期), 对青鳉胚胎发育毒性大小的排序为Hg2+>Pb2+>Cd2+>Cu2+. Hg2+、Cu2+、Pb2+、Cd2+对海水青鳉仔鱼期24h LC50最低值分别为0.018 mg?L-1 (7日龄)、0.48mg?L-1 (8日龄)、1.87mg?L-1 (6日龄)、1.86mg?L-1 (5日龄), 对青鳉仔鱼毒性大小的排序为Hg2+>Cu2+>Cd2+>Pb2+. 由此得出重金属对海水模式鱼的胚胎和仔鱼发育的敏感期, 并经实验得到其最低敏感浓度, 也为进一步开展海水重金属的生物监测提供了参考依据. |
| Abstract: | In order to study the toxicity and sensitivity of heavy metals to marine organisms, the model of marine fish (Oryzias melastigma) was used in this study. A total of 24 hour acute toxicity was assessed in four representative seawater heavy metal pollutants (Cu、Cd、Pb、Hg), including embryonic development and larval growth of Oryzias melastigma. The results showed that the embryonic development stage was very sensitive to the four heavy metal ions, and they all had obvious toxic effects. The lowest 24 hour LC50 values of Hg2+, Cu2+, Pb2+ , and Cd2+ at the 2 hour embryonic developmental stage of Oryzias melastigma were 2.34 μg?L-1 (Phase I), 0.72 mg?L-1 (Phase IV), 22.65 μg?L-1 (Phase I), 27.32 μg?L-1 (Phase I), respectively. The order of embryo development toxicity was Hg2+>Pb2+>Cd2+>Cu2+. The lowest 24 hour LC50 values of Hg2+, Cu2+, Pb2+, and Cd2+ to larval stage of Oryzias melastigma were 0.018 mg?L-1 (7 days old), 0.48 mg?L-1 (8 days old), 1.87 mg?L-1 (6 days old), 1.86 mg?L-1 (5 days old) , respectively. The order of toxicity to Oryzias melastigma larvae was Hg2+ > Cu2+ >Cd2+ >Pb2+. In summary, the sensitive period of heavy metals to the embryonic and larval development of marine model fish was determined in this experiment. The lowest effective concentration was calculated to provide a reference for further biological monitoring of marine heavy metals. |
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| 备注/Memo: | 收稿日期: 2020－12－17. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市自然科学基金（2017A610282）; 教育部重点科技项目（212070）; 宁波大学学科开放基金（XKZSC1508）. 第一作者: 杨志艳（1996－）, 女, 重庆开州人, 在读硕士研究生, 主要研究方向: 环境毒理学. E-mail: 13896919243@163.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/*通信作者: 徐永健（1975－）, 男, 浙江台州人, 教授, 主要研究方向: 动物行为生态与养殖技术. E-mail: xuyongjian@nbu.edu.cn |
