温度和pH对不同日龄蓝点马鲛鱼仔鱼消化酶活性的影响
PDF下载 (443)陈 健1,郑春静2*,桑卫国1.温度和pH对不同日龄蓝点马鲛鱼仔鱼消化酶活性的影响[J].宁波大学学报(理工版),2015,28(03):9-12.DOI:
CHEN Jian1,ZHEN Chun-jing2*,SANG Wei-guo1.Effects of Temperature and pH on Digestive Enzyme Day-varying Activity Larvae of Scomberomorus niphoius[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(03):9-12.DOI:
| Title: | Effects of Temperature and pH on Digestive Enzyme Day-varying Activity Larvae of Scomberomorus niphoius |
| 作者: | 陈 健1, 郑春静2*, 桑卫国1 |
| Author(s): | CHEN Jian1, ZHEN Chun-jing2*, SANG Wei-guo1 |
| 关键词: | 蓝点马鲛鱼; 仔鱼; 消化酶; 温度 |
| Keywords: | pH; Scomberomorus niphoius; larvae; digestive enzyme; temperature |
| 分类号: | S965.3 |
| 文献标识码: | A |
| 摘要: | 用生化方法研究温度和pH对蓝点马鲛鱼仔鱼蛋白酶和淀粉酶活力的影响. 结果表明: 随着pH值的升高, 蓝点马鲛鱼仔鱼的蛋白酶先升高后下降, 淀粉酶先升高后下降. 其中, 1日龄仔鱼蛋白酶最适pH值为8, 7日龄和15日龄的蛋白酶最适pH值为2; 1日龄的淀粉酶最适pH值为7, 7日龄的淀粉酶最适pH值为7~7.5, 15日龄的淀粉酶最适pH值为7.5. 随着温度升高, 蓝点马鲛鱼仔鱼的蛋白酶先升高后下降, 淀粉酶随温度先升高后下降. 其中, 1日龄和7日龄仔鱼的蛋白酶最适温度为37℃, 15日龄的蛋白酶最适温度为42℃; 1日龄的淀粉酶最适温度为42℃, 7日龄的淀粉酶最适温度为37~42℃, 15日龄的淀粉酶最适温度为42℃. |
| Abstract: | Effects of pH and temperature on activities of protease and amylase are studied in Scomberomorus niphoius larvae using enzyme analysis approach. It is found that the activities of protease and amylase first increase in the Scomberomorus niphoius larvae followed by decreasing along with rising pH. The results indicate that the optimal pH of protease for 1st-day, 7th-day and 15th-day larvaes is 8, 2, 2, respectively; The optimal pH of amylase for 1st-day, 7th-day, 15th-day larvaes is 7, 7–7.5, and 7.5, respectively. The activities of protease and amylase in Scomberomorus niphoius larvae are found to first increase and then decrease while temperature is rising. The results also indicate that?the optimal temperature of protease for 1st-day, 7th-day, and 15th-day larvaes is 37℃, 42℃, 42℃, respectively. The optimal temperature of amylase for 1st-day, 7th-day, and 15th-day larvaes is 42℃, 37–42℃, 42℃, respectively. |
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| 备注/Memo: | 收稿日期: 2015?01?26. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市农业科技攻关项目（ 2012C 10035 ） ; 宁波市海洋与渔业专项资金项目（甬海 ﹝ 2014 ﹞ 46 号） . 第一作者: 陈健（1988－）, 男, 浙江宁波人, 在读硕士研究生, 主要研究方向: 水产养殖. E-mail: 327154439@qq.com *通信作者: 郑春静（1965－）, 女, 浙江宁波人, 高级工程师, 主要研究方向: 水产养殖. E-mail: 179109530@qq.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
