几种理化因子对刺激隐核虫包囊及幼虫活力的影响
PDF下载 (514)郑晓叶,刘 顺,周曦,王国良*.几种理化因子对刺激隐核虫包囊及幼虫活力的影响[J].宁波大学学报(理工版),2015,28(01):15-20.DOI:
ZHENG Xiao-ye,LIU Shun,ZHOU Min-xi,WANG Guo-liang*.Influence of Environmental Factors and Chemotherapeutants on Cryptocaryon irritans Tomont and Theront[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(01):15-20.DOI:
| Title: | Influence of Environmental Factors and Chemotherapeutants on Cryptocaryon irritans Tomont and Theront |
| 作者: | 郑晓叶, 刘 顺, 周曦, 王国良* |
| Author(s): | ZHENG Xiao-ye, LIU Shun, ZHOU Min-xi, WANG Guo-liang* |
| 关键词: | 刺激隐核虫; 温度; 盐度; 化学药剂 |
| Keywords: | Cryptocaryon irritans; temperature; salinity; chemotherapeutants |
| 分类号: | S942.2 |
| 文献标识码: | A |
| 摘要: | 选择温度、盐度等环境因子和不同种类及浓度梯度的化学药剂进行单因素试验, 研究其对刺激隐核虫包囊孵化和幼虫活性的影响. 实验结果显示, 温度对刺激隐核虫包囊的孵化具有显著影响; 盐度对刺激隐核虫包囊的孵化和幼虫活性均有显著影响. 选择的高锰酸钾、双氧水、硫酸铜、盐水、福尔马林浸泡药物对包囊或幼虫也均有一定影响, 而采用福尔马林结合低盐度海水进行浸泡处理, 既可有效杀灭包囊和幼虫, 又可减少环境污染并降低生产成本. |
| Abstract: | In this study, the effects of the temperature and salinity on the hatchability of Cryptocaryon irritans tomonts, as well as the cytotoxic effects of different chemotherapeutants on tomonts and theronts were evaluated. The results showed that the hatchability of tomonts was all above 60% in temperatures ranging from 17℃ to 32℃. However, the hatchability of tomonts decreased to 15% at 37℃. The hatchability of tomonts was all above 95% in water salinity range from 15‰-35‰ at 27℃. Interestingly, the highest vitality of the theronts was observed in the groups that the tomonts hatched with salinities being 20‰ and 25‰. The result suggested that the salinity would affect the hatchability of tomonts and the vitality of the theronts. Moreover, some chemotherapeutants exhibited cytotoxic effects on theronts according to the results from this study. It showed that 1-25mg·L-1 CuSO4 could effectively inhibit the hatching of tomonts, but had no killing effects on the theronts. The formalin could either inhibit hatching of tomonts or kill the theronts effectively, and these effectswere positively correlated with the concentrations. Moreover, the low salinity seawater was found to be also able to inhibit the hatching of tomonts and effectively kill the theronts. According to the results from this study, it suggested that the formalin together with the low salinity seawater could be used as a feasible and effective treatment for the infected fishes |
| 参考文献 /References: | [1].Colorni A, Burgess P. Cryptocaryon irritans Brown 1951, the cause of ‘white spot disease’in marine fish: An update[J]. Aquarium Sciences and Conservation, 1997, 1(4):217-238. [2].陈爱平, 江育林, 钱东. 刺激隐核虫病[J]. 中国水产, 2011, 8:39-40. [3].刘振勇, 林小金, 谢友俭, 等. 大黄鱼刺激隐核虫病继发细菌感染致死原因的研究[J]. 福建水产, 2012, 34(1): 11-15. [4].Wilkie D W, Gordin H. Outbreak of cryptocaryoniasis in marine aquaria at scripps institution of oceanography[J]. Calif Fish Game, 1969, 55:227-236. [5].Colorni A. Biology of cryptocaryon irritans and strategies for its control[J]. Aquaculture, 1987, 67(1/2):236-237. [6].Colorni A. Aspects as a control measure in of the biology of cultured gilt-head Cryptocaryon irritans, and hyposalinity sea bream sparus aurata[J]. Dis Aquat lraan, 1985(1): 19-27. [7].Burgess P J, Matthews R A. Cryptocaryon irritans (Ciliophora): Acquired protective immunity in the thick- lipped mullet, Chelon labrosus[J]. Fish & Shellfish Immunology, 1995, 5(6):459-468. [8].滕楠. 刺激隐核虫(Cryptocaryon irritans Brown 1951) 在卵形鲳(Trachinotu ovatus)上的传代及免疫应答反应[D]. 广州: 中山大学, 2005. [9].王印庚, 刘志伟, 林春嫒. 养殖大菱鲆隐核虫病及其治疗[J]. 水产学报, 2011, 35(7):1105-1112. [10].王国良, 周旻曦, 徐益军. 刺激隐核虫 LAMP 检测方法的建立[J]. 中国预防兽医学报, 2013, 35(7):574-577. [11].徐润林, 江静波, 陈毕生. 刺激隐核虫生活史的光镜观察[J]. 海洋科学, 1992(3):42-44. [12].但学明. 刺激隐核虫(Cryptocaryon irritans)的传代、保存及刺激隐核虫病的防治研究[D]. 广州: 暨南大学, 2006. [13].Yambot A V, Song Y L, Sung H H. Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan[J]. Diseases of Aquatic Organisms, 2003, 54(2):147-156. [14].陈小玲, 黄志清. 鱼类感染刺激隐核虫的免疫保护机制[J]. 安徽农业科学, 2009, 37(12):552l-5522. [15].但学明, 李安兴, 林小涛, 等. 卵形鲳鲹对刺激隐核虫的免疫应答和免疫保护研究[J]. 水生生物学报, 2008, 12(1):13-18. [16].Bai J S, Xie M Q, Zhu X Q, et al. Comparative studies on the immunogenicity of theronts, tomonts and trophonts of Cryptocaryon irritans in grouper[J]. Parasitology Research, 2008, 102(2):307-313. [17].Wang F, Li R, Xie M, et al. The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum l-amino acid oxidase is isolated[J]. Fish & Shellfish Immunology, 2011, 30(4):1095-1108. [18].邱军强, 刘玮, 杨先乐, 等. 刺激隐核虫免疫学研究进展[J]. 中国病原生物学杂志, 2011, 6(10):787-788. [19].Mohd-Shaharuddin N, Mohd-Adnan A, Chu K B, et al. Expression profile of immune-related genes in lates calcarifer infected by Cryptocaryon irritans[J]. Fish & Shellfish Immunology, 2013, 34 (3):762-769. [20].Khoo C K, Abdul-Murad A M, Kua B C, et al. Cryptocaryon irritans infection induces the acute phase response in lates calcarifer: A transcriptomic perspective [J]. Fish & Shellfish Immunology, 2012, 33(4):788-794. [21].Hirazawa N, Goto T, Shirasu K. Killing effect of various treatments on the monogenean heterobothrium okamotoi eggs and oncomiracidia and the ciliate Cryptocaryon irritans cysts and theronts[J]. Aquaculture, 2003, 223(1): 1-13. [22].Cheung P J, Nigrelli R F, Ruggieri G D. Studies on cryptocaryoniasis in marine fish: Effect of temperature and salinity on the reproductive cycle of Cryptocaryon irritans Brown, 1951[J]. Journal of Fish Diseases, 1979, 2(2):93-97. [23].Burgess P J, Matthews R A. Cryptocaryon irritans (Ciliophora): Photoperiod and transmission in marine fish[J]. Mar Biol Assoc UK, 1994(74):535-542. [24].言颖秀. 刺激隐核虫(Cryptocaryon irritans Brown 1951) 生物学特性的研究[D]. 2005. [25].Dan X M, Li A X, Lin X T, et al. A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus[J]. Aquaculture, 2006, 258(1): 127-133. [26].俞开康, 战文斌, 周丽. 海水养殖病害诊断与防治手册[M]邱彦涛, 宋微波. 3种药物对扇形游仆虫Euplotes vannus(纤毛门, 寡毛目)的急性毒性作用应用与环境生物学报626. [27].邱彦涛, 宋微波. 3Tojo J, Santamarina M T. Oral pharmacological treat- ments for parasitic diseases of rainbow trout Oncorhynchus mykiss[J]. I Hexamita Salmonis Dis Aquat Org, 1998, 33(1):51-56. [28].闫茂仓, 邵鑫斌, 单乐州, 等. 福尔马林防治 Miichthys miiuy (Basilewsky)刺激隐核虫(Cryptocaryon irritans)的研究[J]. 现代渔业信息, 2008, 23(11):16-19.nfluence of Environmental Factors and Chemotherapeutants on Cryptocaryon irritans Tomont and Theront [29].ZHENG Xiao-ye, LIU Shun, ZHOU Min-xi, WANG Guo-liang* [30].( Key Laboratory of Applied Technology of Marine Biology of Ministry of Education, Ningbo University, Ningbo 315211, China ) [31].Abstract: In this study, the effects of the temperature and salinity on the hatchability of Cryptocaryon irritans tomonts, as well as the cytotoxic effects of different chemotherapeutants on tomonts and theronts were evaluated. The results showed that the hatchability of tomonts was all above 60% in temperatures ranging from 17℃ to 32℃. However, the hatchability of tomonts decreased to 15% at 37℃. The hatchability of tomonts was all above 95% in water salinity range from 15‰-35‰ at 27℃. Interestingly, the highest vitality of the theronts was observed in the groups that the tomonts hatched with salinities being 20‰ and 25‰. The result suggested that the salinity would affect the hatchability of tomonts and the vitality of the theronts. Moreover, some chemotherapeutants exhibited cytotoxic effects on theronts according to the results from this study. It showed that 1-25mg·L-1 CuSO4 could effectively inhibit the hatching of tomonts, but had no killing effects on the theronts. The formalin could either inhibit hatching of tomonts or kill the theronts effectively, and these effectswere positively correlated with the concentrations. Moreover, the low salinity seawater was found to be also able to inhibit the hatching of tomonts and effectively kill the theronts. According to the results from this study, it suggested that the formalin together with the low salinity seawater could be used as a feasible and effective treatment for the infected fishes. |
| 备注/Memo: | 收稿日期: 2013?12?17. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 教育部长江学者与创新团队项目（IRT0734）; 农业部公益性行业科研专项（200903029）; 国家星火重大项目（2011GA701001）;宁波市科技创新团队项目（2013B82012）. 第一作者: 郑晓叶（1988－）, 女, 河南叶县人, 在读硕士研究生, 主要研究方向: 鱼病学研究. E-mail: 819053744@qq.com *通信作者: 王国良（1955－）, 男, 浙江舟山人, 博导/教授, 主要研究方向: 鱼病学研究. E-mail: wangguoliang@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
