贪食迈阿密虫的体外培养及有效抗虫药物筛选
PDF下载 (479)刘 璐,韦昊麟,王国良,蒋霞敏,周素明,陶 震 *.贪食迈阿密虫的体外培养及有效抗虫药物筛选[J].宁波大学学报(理工版),2017,30(2):11-14.DOI:
LIU Lu,WEI Hao-lin,WANG Guo-liang,JIANG Xia-min,ZHOU Su-ming,TAO Zhen *.In vitro culture of fish pathogen Miamiensis avidus and screening of effective antiprotozoals[J].Journal of Ningbo University(Natural Science & Engineering Edition),2017,30(2):11-14.DOI:
| Title: | In vitro culture of fish pathogen Miamiensis avidus and screening of effective antiprotozoals |
| 作者: | 刘 璐, 韦昊麟, 王国良, 蒋霞敏, 周素明, 陶 震 * |
| Author(s): | LIU Lu, WEI Hao-lin, WANG Guo-liang, JIANG Xia-min, ZHOU Su-ming, TAO Zhen * |
| 关键词: | 贪食迈阿密虫; 体外培养; 抗虫药物 |
| Keywords: | Miamiensis avidus; in vitro culture; temperature; antiprotozoal drug |
| 分类号: | S917.1 |
| 文献标识码: | A |
| 摘要: | 贪食迈阿密虫(Miamiensis avidus)是一种已知的养殖鱼类兼性寄生虫, 可严重危害养殖水产动物的健康并导致死亡. 为了研究有效的贪食迈阿密虫防治方法, 以从虎斑乌贼溃疡病患处分离的一株贪食迈阿密虫(SP-1株)为实验对象, 设计了一种贪食迈阿密虫的体外培养方法, 结果显示在最适生长温度为20℃, 培养至稳定期时虫体密度可达1×105个?mL-1. 采用体外浸浴模型分析了阿苯达唑、盐酸强力霉素、甲硝唑、硫酸奎宁、盐酸左旋咪唑、福尔马林和双氧水等药物对SP-1株的杀灭效果. 结果表明, SP-1株对硫酸奎宁、盐酸左旋咪唑的敏感性较高, 24h浸浴最小致死质量浓度分别为12.5μg?mL-1和25μg?mL-1; 体积浓度为50μL?L-1的福尔马林溶液或双氧水均可在1h内完全杀灭暴露在其中的SP-1株. |
| Abstract: | Miamiensis avidus is a known facultative fish parasite. This histophagous ciliate has been reported to infest several mariculture fish and cause a high mortality level. In the present study, we successfully cultured a M. avidus strain SP-1 isolated from skin ulcers of a captive Sepia pharaonis. The results of culture experiments showed that the optimal growth temperature was 20℃ and the ciliate was able to grow to 105 cells?mL-1 in the medium. To develop prophylactic strategies for M. avidus infections, seven candidate antiprotozoals were screened for activity against this pathogen in vitro. Five of these were found potent in the antiprotozoal effect measured by quinine sulfate (24-h minimum lethal concentration; MLC-24=12.5mg?L-1), levamisole hydrochloride (MLC-24=25.0mg?L-1), albendazole (MLC-24=100.0mg?L-1), hydrogen peroxide (1-h lethal concentration MLC-1=50.0mg?L-1) and formalin (MLC-1=50.0mg?L-1). Our findings provide supporting evidence about parasitic ciliate infection prevention in aquaculture. |
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| 备注/Memo: | 收稿日期: 2016－10－28. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省重中之重学科开放基金（xkzsc1403）; 宁波市农业科技专项（2014C11001）. 第一作者: 刘璐（1992－）, 女, 河北定州人, 在读硕士研究生, 主要研究方向: 水产动物病害防控. E-mail: 719155053@qq.com *通信作者: 陶震（1985－）, 男, 浙江金华人, 讲师, 主要研究方向: 水产动物病害防控. E-mail: taozhen@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
