Cole-Cole方程参数对细胞介电频谱的影响
PDF下载 (373)张 倩 1,方 云 1,李会英 2,汤治元 1,赵伟红 1,马 青 1*.Cole-Cole方程参数对细胞介电频谱的影响[J].宁波大学学报(理工版),2014,27(04):115-121.DOI:
ZHANG Qian 1,FANG Yun 1,LI Hui-ying 2,TANG Zhi-yuan 1,ZHAO Wei-hong 1,MA Qing 1*.Influence of Cole-Cole Equation Parameters on Dielectric Spectrum of Cell[J].Journal of Ningbo University(Natural Science & Engineering Edition),2014,27(04):115-121.DOI:
| Title: | Influence of Cole-Cole Equation Parameters on Dielectric Spectrum of Cell |
| 作者: | 张 倩 1, 方 云 1, 李会英 2, 汤治元 1, 赵伟红 1, 马 青 1* |
| Author(s): | ZHANG Qian 1, FANG Yun 1, LI Hui-ying 2, TANG Zhi-yuan 1, ZHAO Wei-hong 1, MA Qing 1* |
| 关键词: | Cole-Cole方程; 介电频谱; 介电损耗因子; 损耗角正切; Cole-Cole图 |
| Keywords: | Cole-Cole equation; dielectric spectrum; loss factor; loss tangent; Cole-Cole plot |
| 分类号: | Q64 |
| 文献标识码: | A |
| 摘要: | 为探讨Cole-Cole方程8个参数(Δε1, Δε2, εh, κl, f1, f2, β1, β2)对细胞介电频谱的影响, 采用改变单个参数, 固定其他参数的方法, 观察介电频谱的变化. 结果显示: Δε1影响低频介电频谱和Cole-Cole(ε′)图; Δε2影响高频介电频谱、Cole-Cole(ε′)图和Cole-Cole(κ′)图; εh影响ε′(f)、Cole-Cole(ε′)图和tgδ(f); κl对κ′(f)和Cole-Cole(κ′)图有影响; f1影响低频介电频谱; f2影响高频介电频谱; β1影响Cole-Cole(ε′)和tgδ(f); β2对高频介电频谱有影响. 可见Cole-Cole方程作为细胞介电频谱的数学模型, 其参数变化有规律可循. |
| Abstract: | In order to investigate the effect of the eight parameters (Δε1, Δε2, εh, κl, f1, f2, β1, β2) of the Cole-Cole formula on the dielectric spectrum of cell, the changing behaviors of the dielectric spectrum are observed as one parameter varies while the other parameters are fixed. Found from the results are that the parameter Δε1 mainly affects the low-frequency dielectric parameters and the Cole-Cole(ε′) plot. The parameter Δε2 has some effects mainly on the high-frequency dielectric parameters and the Cole-Cole(ε′) plot as well as the Cole-Cole(κ′) plot. The ε′(f), Cole-Cole(ε′) plot and tgδ(f) are determined principally by the parameter εh. The parameter κl influences the κ′(f) and Cole-Cole(κ′) plot. The low-frequency dielectric spectra is mainly determined by the first frequency f1. The second frequency f2 affects the high-frequency dielectric spectra. The Cole-Cole(ε′) plot and tgδ(f) are mainly induced by the parameter β1. Changing the value of β2 will lead to the changes in the high-frequency dielectric spectra. It is obvious that the Cole-Cole equation can be regarded as the mathematical model of the dielectric spectra of cell, and the variation of its parameters observes specific rules. |
| 参考文献 /References: | [1] Heileman K, Daoud J, Tabrizian M. Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis[J]. Biosens Bio- electron, 2013, 49(11):348-359. [2] Massimi M, Stampella A, Devirgiliis L C, et al. Dielectric characterization of hepatocytes in suspension and embedded into two different polymeric scaffolds[J]. Colloids and Surfaces B, 2013, 102:700-707. [3] Asami K. Dielectric spectroscopy reveals nanoholes in erythrocyte ghosts[J]. Soft Matter, 2012, 8(11):3250- 3257. [4] Wolf M, Gulich R, Lunkenheimer P, et al. Broadband dielectric spectroscopy on human blood[J]. Biochim Biophys Acta, 2011, 1810(8):727-740. [5] Biasio D, Cametti C. On the dielectric relaxation of biological cell suspensions: The effect of the membrane electrical conductivity[J]. Colloids and Surfaces B, 2011, 84(2):433-441. [6] 王力, 马青, 陈林, 等. 人血液介电谱Cole-Cole数学模型的解析[J]. 中国生物医学工程学报, 2010, 29(2), 253-258. [7] 马青, 王力, 陈林. 大鼠腓肠肌细胞介电谱测量和Cole-Cole数学模型分析[J]. 中国生物医学工程学报, 2009, 28(5):686-690. [8] 于艳丽, 赵孔双, 白伟, 等. 金属离子及CTAB对酵母细胞介电行为的影响-相互作用的模型化解析[J]. 生物物理学报, 2008, 24(5):348-360. [9] Daoud J, Asami K, Rosenberg L, et al. Dielectric spectroscopy for non-invasive monitoring of epithelial cell differentiation within three-dimensional scaffolds[J]. Phys Med Biol, 2012, 57(16):5097-5112. [10] Stacey M W, Sabuncu A C, Beskok A. Dielectric characterization of costal cartilage chondrocytes[J]. Biochim Biophys Acta, 2014, 1840(1):146-152. [11] Cole K S, Cole R H. Dispersion and absorption in dielectrics I: Alternating current characteristics[J]. Journal of Chemical Physics, 1941, 9(4):341-351. |
| 备注/Memo: | 收稿日期: 2014-03-08. 宁波大学学报(理工版)网址: http://journallg.nbu.edu.cn/基金项目: 国家自然科学基金(51277099); 宁波市自然科学基金(2012A610211, 2013A610211); 浙江省新苗人才计划(2013R405060).第一作者: 张倩(1991-), 女, 河南驻马店人, 在读硕士研究生, 主要研究方向: 细胞介电谱的理论模型. E-mail: ningbozhangqian@163.com*通信作者: 马青(1962-), 男, 黑龙江佳木斯人, 教授, 主要研究方向: 细胞生物物理. E-mail: maqing@nbu.edu.cn 宁波大学学报(理工版)网址:http://journallg.nbu.edu.cn/ |