氨基功能化大孔SiO2固定化漆酶
PDF下载 (431)张育淇 1,2,刘晓贞 1,2,李 云 1,2,梁云霄 1,2*.氨基功能化大孔SiO2固定化漆酶[J].宁波大学学报(理工版),2015,28(02):108-113.DOI:
ZHANG Yu-qi 1,2,LIU Xiao-zhen 1,2,LI Yun 1,2,LIANG Yun-xiao 1,2*.Immobilization of Laccase on Amino-functionalized Macroporous Silica[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(02):108-113.DOI:
| Title: | Immobilization of Laccase on Amino-functionalized Macroporous Silica |
| 作者: | 张育淇 1, 2, 刘晓贞 1, 2, 李 云 1, 2, 梁云霄 1, 2* |
| Author(s): | ZHANG Yu-qi 1, 2, LIU Xiao-zhen 1, 2, LI Yun 1, 2, LIANG Yun-xiao 1, 2* |
| 关键词: | 大孔SiO2; 固定化; 漆酶; 氨基化修饰 |
| Keywords: | macroporous silica; immobilization; laccase; amino-modified |
| 分类号: | Q814.2 |
| 文献标识码: | A |
| 摘要: | 在溶剂热条件下, 采用后嫁接法对新型大尺寸SiO2大孔材料进行氨基化改性, 以氨基修饰的SiO2大孔材料为载体, 借助交联剂戊二醛(GA)使诺维信工业漆酶固定化. 研究结果表明: 在GA质量分数为3%, pH值为5, 漆酶质量浓度为30mg?mL-1条件下固定化4h制备得到的固定化漆酶最优, 活力达到111.4U?g-1; 比较固定化漆酶和游离漆酶的性质发现, 固定化漆酶pH稳定性和热稳定性均优于游离漆酶, 固定化漆酶重复使用性好, 与底物反复操作10次后, 相对活性仍保持70.8%. |
| Abstract: | A novel large-sized macroporous silica material is amino-modified via post-grafting method under solvothermal conditions. The amino-functionalized macroporous silica is employed as the supports for immobilization of the commercial Novozymes laccase by using glutaraldehyde (GA) as the crosslinking agent. The results show that at the concentration of 3% glutaraldehyde, immobilizing time of 4 h, the buffer of pH 5.0 and the initial laccase concentration of 30 mg?mL-1, the activity of immobilized laccase reaches up to 111.4 U?g-1. Compared with the free counterpart, it is found that the pH and thermal stabilities of the immobilized laccase are improving significantly, and the immobilized laccase retaines 70.8% of its initial activity after ten repetitive times of catalytic reaction, indicating that the immobilized laccase has a good reusability. |
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| 备注/Memo: | 收稿日期: 2014－01－06. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 浙江省自然科学基金（LY12B01004）; 宁波市社会发展项目（2011C50052）; 宁波大学王宽诚幸福基金.第一作者: 张育淇（1987－）, 男, 广西北流人, 在读硕士研究生, 主要研究方向: 无机功能材料. E-mail: jacketzhangyq@hotmail.com*通信作者: 梁云霄（1965－）, 男, 黑龙江齐齐哈尔人, 教授, 主要研究方向: 无机功能材料. E-mail: liangyunxiao@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
