基于磁性分子印迹胶体磁珠分离和GC-MS检测血清中脂联素
PDF下载 (346)胡富陶,余红卫.基于磁性分子印迹胶体磁珠分离和GC-MS检测血清中脂联素[J].宁波大学学报(理工版),2015,28(02):103-107.DOI:
HU Fu-tao,YU Hong-wei.Enrichment of Serum Adiponectin and Separation Based on Magneticmolecularly Imprinted Colloidal Beads[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(02):103-107.DOI:
| Title: | Enrichment of Serum Adiponectin and Separation Based on Magneticmolecularly Imprinted Colloidal Beads |
| 作者: | 胡富陶, 余红卫 |
| Author(s): | HU Fu-tao, YU Hong-wei |
| 关键词: | 分子印迹; 胶体磁珠; 血清脂联素; 特异性富集 |
| Keywords: | molecular imprinting; magnetic immune-beads; serum adiponectin; specific enrichment |
| 分类号: | O657 |
| 文献标识码: | A |
| 摘要: | 采用分子印迹技术, 以3,4-二氯苯乙酸(DCP)作为模板分子合成了MWCNTs@DMMIPs材料, 克服了脂联素(ADPN)质量浓度低, 不易形成氢键等缺点, 增强了材料的吸附能力. 实验对血类中常见的ADPN用GC-MS方法进行检测, 检测限为0.32μg?mL-1, 回收率为83.2%~101.2%. 材料适用于血类中常见的ADPN检测. |
| Abstract: | In this paper, we use the molecular imprinting technique to enrich the serum adiponectin for detection purposes. MWCNTs@DMMIPs material is synthesized using DCP astemplate molecule, which can overcome the shortcomings of lower ADPN concentration, and difficulty to form hydrogen bonds and others. Using the proposed method, the adsorption capacity of the material can be enhanced. Six kinds of common ADPN blood type in the course of the experiment are detected by GC-MS method. It is found that the detection is 0.32 μg?mL-1, the recovery rate between 83.2%-101.2%. Detection material is found to be suitable for enriching the common ADPN in blood. |
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| 备注/Memo: | 收稿日期: 2014－10－10. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 浙江省公益技术应用研究（2012C37012）.第一作者: 胡富陶（1964－）, 男, 浙江磐安人, 高级实验师, 主要研究方向: 食品安全与检测. E-mail: hufutao@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
