新型分子印迹荧光传感器对海水中邻苯二甲酸二(2-乙基)己酯的快速检测技术研究
PDF下载 (381)潘俏芬,尤金杰,刘 华,陆 茵,孙爱丽,张泽明.新型分子印迹荧光传感器对海水中邻苯二甲酸二(2-乙基)己酯的快速检测技术研究[J].宁波大学学报(理工版),2022,35(6):98-104.DOI:
PAN Qiaofen,YOU Jinjie,LIU Hua,LU Yin,SUN Aili,ZHANG Zeming.A novel molecularly imprinted fluorescence sensor for rapid detection of bis-(2-ethylhexyl) phthalate in seawater[J].Journal of Ningbo University(Natural Science & Engineering Edition),2022,35(6):98-104.DOI:
| Title: | A novel molecularly imprinted fluorescence sensor for rapid detection of bis-(2-ethylhexyl) phthalate in seawater |
| 作者: | 潘俏芬, 尤金杰, 刘 华, 陆 茵, 孙爱丽, 张泽明 |
| Author(s): | PAN Qiaofen, YOU Jinjie, LIU Hua, LU Yin, SUN Aili, ZHANG Zeming |
| 关键词: | 邻苯二甲酸二(2-乙基)己酯; 分子印迹; 量子点; 传感器 |
| Keywords: | bis-(2-ethylhexyl) phthalate; molecular imprinting; quantum dots; sensor |
| 分类号: | X834 |
| 文献标识码: | A |
| 摘要: | 构建对邻苯二甲酸二(2-乙基)己酯(Bis(2-ethylhexyl) phthalate, DEHP)具有特异荧光响应的分子印迹-量子点传感器, 应用于实际样品中DEHP的残留检测. 采用改进的反相微乳液法, 制备了对DEHP具有较高选择性和灵敏性响应的分子印迹-量子点纳米结构聚合物(MIP-QDs), 通过扫描电镜、红外光谱、X射线光电子能谱、选择性分析等方法对获得的MIP-QDs理化特性进行分析, 成功将印迹层锚定在QDs上. 进一步通过响应体系优化, 结果表明, 构建的MIP-QDs在V (水)V (乙醇)=82、pH为8.0体系中, 对DEHP具有较高的选择性响应; 并在0.005~25.0mg·L-1的DEHP质量浓度范围内, DEHP质量浓度与荧光猝灭效率()间存在良好线性关系, 相关系数为0.9901, 检测限低至1.5?g·L-1, 加标回收率为92.0%~96.8%, 相对标准偏差低于8.2%; 且在实际海水检测与GC-MS/MS检测结果之间具有良好的一致性. 充分表明本文构建的MIP-QDs荧光传感器可用于海水中DEHP的实时定量检测. |
| Abstract: | A molecularly imprinted polymer based on bis-(2-ethylhexyl) phthalate (DEHP) was prepared by an improved reverse micro emulsion method. A molecularly imprinted quantum dot fluorescence sensor (MIP-QDs) with specific fluorescence response to DEHP was constructed by embedding CdSe/ZnS quantum dots. The morphology of MIP-QDs was analyzed by SEM, FT-IR and X-ray photoelectron spectroscopy, and the detection system was optimized on this basis. The results showed that MIP-QDs had a high selective response to DEHP in V(water)V(ethanol)=82 and pH 8.0 system, and had a good linear relationship with in the range of DEHP concentration from 0.005-25.0 mg·L-1, with the correlation coefficient being 0.9901 and the detection limit being as low as 1.5 μg·L-1. In actual seawater, the spiked recoveries of MIP-QDs ranged from 92.0%- 96.8%, and the RSD was lower than 8.21%. There was no significant difference between MIP-QDs and GC-MS/MS in actual seawater detection, and the accuracy rate was not lower than 87.23%. In conclusion, MIP-QDs fluorescence sensor constructed in this paper can be used for real-time detection of DEHP in Marine water. |
| 参考文献 /References: | [1] 王笑妍, 薛燕波, 者东梅, 等. 邻苯二甲酸酯类增塑剂概况及法规标准现状[J]. 中国塑料, 2019, 33(6):95- 105. [2] 宋雪英, 崔小维, 李嘉康, 等. 邻苯二甲酸酯类塑化剂的土壤生态毒理学研究进展[J]. 生态环境学报, 2016, 25(11):1885-1890. [3] 张璐璐, 刘静玲, 何建宗, 等. 中国典型城市水环境中邻苯二甲酸酯类污染水平与生态风险评价[J]. 生态毒理学报, 2016, 11(2):421-435. [4] 刘乃榕, 王少鹏, 杨光. 邻苯二甲酸酯类毒性作用研究进展[J]. 大连医科大学学报, 2016, 38(5):492-495. [5] Hu H M, Mao L L, Fang S H, et al. Occurrence of phthalic acid esters in marine organisms from Hangzhou Bay, China: Implications for human exposure[J]. Science of the Total Environment, 2020, 721:137605. [6] Cao X L. Phthalate esters in foods: Sources, occurrence, and analytical methods[J]. Comprehensive Reviews in Food Science and Food Safety, 2010, 9(1):21-43. [7] Paluselli A, Aminot Y, Galgani F, et al. Occurrence of phthalate acid esters (PAEs) in the northwestern Mediterranean Sea and the Rhone River[J]. Progress in Oceanography, 2018, 163:221-231. [8] Wu P G, Pan X D, Ma B J, et al. Determination of phthalate esters in non-alcoholic beverages by GC-MS and optimization of the extraction conditions[J]. European Food Research and Technology, 2014, 238(4): 607-612. [9] 孙海燕, 王炎. 顶空固相微萃取-气相色谱法测定辣椒根系分泌物中邻苯二甲酸酯类化合物[J]. 理化检验: 化学分册, 2014, 50(4):447-451. [10] 王风红, 芦春梅, 胡婷婷, 等. 高效液相色谱-串联质谱法测定化妆品中的邻苯二甲酸酯[J]. 化学试剂, 2015, 37(2):135-138. [11] Yu J L, Wang X Y, Kang Q, et al. One-pot synthesis of a quantum dot-based molecular imprinting nanosensor for highly selective and sensitive fluorescence detection of 4-nitrophenol in environmental waters[J]. Environmental Science: Nano, 2017, 4(2):493-502. [12] Whitcombe M J, Chianella I, Larcombe L, et al. The rational development of molecularly imprinted polymer- based sensors for protein detection[J]. Chemical Society Reviews. 2011, 40(3):1547-1571. [13] Wang X Y, Yu S M, Liu W, et al. Molecular imprinting based hybrid ratiometric fluorescence sensor for the visual determination of bovine hemoglobin[J]. ACS Sensors, 2018, 3(2):378-385. [14] Wang X Y, Yu J L, Wu X Q, et al. A molecular imprinting-based turn-on Ratiometric fluorescence sensor for highly selective and sensitive detection of 2,4-dichlorophenoxyacetic acid (2,4-D) [J]. Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices, 2016, 81:438-444. [15] Sistani S, Shekarchizadeh H. Fabrication of fluorescence sensor based on molecularly imprinted polymer on amine-modified carbon quantum dots for fast and highly sensitive and selective detection of tannic acid in food samples[J]. Analytica Chimica Acta, 2021, 1186(22): 339122. [16] Mehrzad-Samarin M, Faridbod F, Dezfuli A S, et al. A novel metronidazole fluorescent nanosensor based on graphene quantum dots embedded silica molecularly imprinted polymer[J]. Biosensors and Bioelectronics, 2017, 92:618-623. [17] Zhang Z M, Wang L Y, Gu Y Y, et al. Probing the contamination characteristics, mobility, and risk assessments of typical plastic additive-phthalate esters from a typical coastal aquaculture area, China[J]. Journal of Hazardous Materials, 2021, 416:125931. [18] Liu C X, Zhao J, Zhang R R, et al. Development and application of fluorescence sensor and test strip based on molecularly imprinted quantum dots for the selective and sensitive detection of propanil in fish and seawater samples[J]. Journal of Hazardous Materials, 2020, 389: 121884. [19] Zhou Z P, Li T, Xu W Z, et al. Synthesis and characterization of fluorescence molecularly imprinted polymers as sensor for highly sensitive detection of dibutyl phthalate from tap water samples[J]. Sensors and Actuators B: Chemical, 2017, 240:1114-1122. [20] Zhou Y, Qu Z B, Zeng Y B, et al. A novel composite of graphene quantum dots and molecularly imprinted polymer for fluorescent detection of paranitrophenol[J]. Biosensors and Bioelectronics, 2014, 52:317-323. [21] Wei X, Zhou Z P, Hao T F, et al. Molecularly imprinted polymer nanospheres based on Mn-doped ZnS QDs via precipitation polymerization for room-temperature phosphorescence probing of 2,6-dichloropheno [J]. RSC Advances, 2015, 5(26):19799-19806. |
| 备注/Memo: | 收稿日期:2022-03-04.宁波大学学报（理工版）网址:http://journallg.nbu.edu.cn/ 基金项目:浙江省重点研发计划项目（2021C02062）;宁波市公益性计划项目（2022S163,2021S065）;宁波市重点研发项目（2021Z056）. 第一作者:潘俏芬（1996－）,女,广东中山人,在读硕士研究生,主要研究方向:水产品安全与检测.E-mail:397278301@qq.com *通信作者:孙爱丽（1978－）,女,山东潍坊人,博士/高级实验师,主要研究方向:环境健康与水产品安全.E-mail:sunaili@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
