污水中环丙沙星对活性污泥胞外蛋白质组学的影响研究
PDF下载 (253)张佳璐,郭敏辉,吕 阳,于 洁,王 侃,杜志敏.污水中环丙沙星对活性污泥胞外蛋白质组学的影响研究[J].宁波大学学报(理工版),2023,36(4):48-63.DOI:10.20098/j.cnki.1001-5132.2022.1209
ZHANG Jialu,GUO Minhui,L? Yang,YU Jie,WANG Kan,DU Zhimin.Effect of ciprofloxacin in wastewater on extracellular proteomics of activated sludge[J].Journal of Ningbo University(Natural Science & Engineering Edition),2023,36(4):48-63.DOI:10.20098/j.cnki.1001-5132.2022.1209
| Title: | Effect of ciprofloxacin in wastewater on extracellular proteomics of activated sludge |
| 作者: | 张佳璐, 郭敏辉, 吕 阳, 于 洁, 王 侃, 杜志敏 |
| Author(s): | ZHANG Jialu, GUO Minhui, L? Yang, YU Jie, WANG Kan, DU Zhimin |
| 关键词: | 活性污泥; 环丙沙星; 蛋白质组学; 胞外蛋白质; 结合 |
| Keywords: | activated sludge; ciprofloxacin; proteomics; extracellular proteins; binding |
| 分类号: | Q89 |
| DOI: | 10.20098/j.cnki.1001-5132.2022.1209 |
| 文献标识码: | A |
| 摘要: | 为了考察活性污泥法处理含环丙沙星(Ciprofloxacin, CIP)废水时污泥与CIP的相互作用, 本文采用液相色谱-串联质谱研究CIP影响下活性污泥中胞外蛋白质的表达谱, 并进行基因本体注释分析. 在未投加CIP的对照组和CIP组中分别鉴定出高可信度蛋白质89和97种, 相同表达蛋白质仅13种, 差异显著. 对照组和CIP组胞外蛋白质具有相似的等电点分布, CIP组大分子量胞外蛋白质增多. 从亚细胞定位来看, 对照组和CIP组分别有62.9%和66.0%的蛋白质细胞定位已知, 说明大部分胞外蛋白质源于细胞裂解释放, CIP加入后胞外蛋白质发生了更新和重组, 为CIP的吸附提供了结合位点; CIP的加入导致定位于细胞核的蛋白质显著减少. 从分子功能和生物学过程来看, CIP的加入抑制了与DNA和RNA的结合、合成、转录等分子功能相关的蛋白质的表达, 阻碍了DNA和RNA的转录、分解代谢等生物学过程; 激发了具有核酸修复、蛋白质结合和架桥等分子功能的蛋白质的表达, 促进了核酸损伤修复、应急反应、萜类化合物合成、蛋白质合成和代谢等生物学过程. CIP抑制了对照组中高丰度蛋白质的表达. 可见, CIP通过影响活性污泥的生物学过程引起胞外蛋白质性质的改变, 进而影响活性污泥与CIP的结合. |
| Abstract: | Extracellular proteins in activated sludge play a key role in ciprofloxacin (CIP) removal during the biological wastewater treatment process. The properties of extracellular proteins might be altered by CIP via the biological processes of activated sludge. The effect of CIP on the expression profile of extracellular proteins in activated sludge was investigated in the present study, using liquid chromatography tandem mass spectrometry. In addition, gene ontology annotation analysis was carried out. A total of 89 and 97 of high confidence proteins were identified in the control group and CIP group respectively. A total of 13 proteins were identically expressed, indicating the significant difference between the two groups. The extracellular proteins in the control group and CIP group had similar isoelectric point distribution, which was mainly between 4.5-7.0 and 8.0-10.0. The molecular weight of extracellular proteins was mainly between 10-60kDa and the extracellular proteins with large molecular weight were increased with the addition of CIP. For the subcellular localization, 62.9% and 66.0% of the proteins were present in the control group and CIP group respectively. Cellular localization was identified, including the cytoplasm, nucleus, cell membrane, and various organelle. The results suggest that most of the extracellular proteins were originated from cell lysis. They were also renewed and reorganized after the CIP addition, which is favorable for CIP adsorption. CIP resulted in a significant reduction of proteins located in the nucleus. For the molecular functions and biological processes, the addition of CIP inhibited the expression of proteins related to the binding, synthesis, and transcription of DNA and RNA. It also hindered the transcription and catabolism of DNA and RNA while stimulated the expression of proteins with molecular functions such as nucleic acid repair, proteins binding and bridging. The process promoted the biological processes including nucleic acid damage repair, emergency response, terpene synthesis, proteins synthesis and metabolism. CIP inhibited the expression of high abundance proteins in the control group, such as the 50S ribosomal protein L11 derived from Bartonella bacilliformis, the ferric uptake regulation protein derived from Klebsiella pneumoniae, the RNA polymerase sigma factor SigA derived from Mycobacterium bovis, and the ribonuclease Z 1 from Schizosaccharomyces pombe. CIP could change the properties of extracellular proteins by influencing the biological processes of activated sludge and further promote the binding of activated sludge with CIP. |
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| 备注/Memo: | 收稿日期: 2022－12－09. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 国家自然科学基金（51408325）; 浙江省自然科学基金（Y19E080023）.第一作者: 张佳璐（1998－）, 女, 浙江宁波人, 在读硕士研究生, 主要研究方向: 环境科学与资源利用. E-mail: 903013696@qq.com*通信作者: 王侃（1977－）, 男, 浙江宁波人, 博士/副教授, 主要研究方向: 水污染控制. E-mail: wangkan@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
