交配与未交配三疣梭子蟹肝胰腺蛋白质组学的初步分析
PDF下载 (597)徐 洲,母昌考,李荣华,宋微微,王春琳.交配与未交配三疣梭子蟹肝胰腺蛋白质组学的初步分析[J].宁波大学学报(理工版),2013,26(04):1-6.DOI:
XU Zhou,MU Chang-kao,LI Rong-hua,SONG Wei-wei,WANG Chun-lin.Comparative Proteomics of Hepatopancreas in Mated and Unmated Marine Crab Portunus trituberculatus[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,26(04):1-6.DOI:
| Title: | Comparative Proteomics of Hepatopancreas in Mated and Unmated Marine Crab Portunus trituberculatus |
| 作者: | 徐 洲, 母昌考, 李荣华, 宋微微, 王春琳 |
| Author(s): | XU Zhou, MU Chang-kao, LI Rong-hua, SONG Wei-wei, WANG Chun-lin |
| 关键词: | 三疣梭子蟹; 蛋白质组学; 交配; 肝胰腺 |
| Keywords: | Portunus trituberculatus; proteomics; mating; hepatopancreas |
| 分类号: | Q513 |
| 文献标识码: | A |
| 摘要: | 采用双向电泳和基质辅助激光解析串联飞行时间质谱(MALDI-TOF/TOF)研究三疣梭子蟹交配与未交配雌性个体肝胰腺蛋白质表达. 使用PDQuest软件分析双向电泳凝胶图像, 结果显示: 交配组和未交配组凝胶图像上分别能检测到(400±25)和(530±16)个蛋白点, 其中共有15个蛋白点表达量有显著性差异(P＜0.05). 与未交配组相比, 交配组三疣梭子蟹肝胰腺中6个蛋白点的表达量显著性上升. 经质谱鉴定为: 磷酸丙糖异构酶、磷酸丙酮酸水合酶、过氧化氢酶、δ-谷胱甘肽S-转移酶、ATP合酶α-亚基和胰凝乳蛋白酶. 此外, 交配组三疣梭子蟹肝胰腺中9个蛋白点的表达量显著性下降. 经质谱鉴定为: 延伸因子2、长链酰基CoA脱氢酶、假血蓝蛋白1、假血蓝蛋白2、异柠檬酸脱氢酶、线粒体乙醛脱氢酶X、转酮醇酶、血蓝蛋白α-亚基和腺苷高半胱氨酸酶A. 该结果可为揭示三疣梭子蟹卵巢发育机理提供重要参考. |
| Abstract: | Two-dimensional electrophoresis and matrix-assisted laser desorption ionization tandem time of flight mass spectrometry (MALDI-TOF/TOF) are used to analyze proteomics of hepatopancreas in mated and unmated female Portunus trituberculatus. The two-dimensional electrophoresis gel images analyzed using PDQuest software demonstrate that 15 spots are significantly distinctive. The quantitative expression of 6 spots is incremented in hepatopancreas in the mated group which is identified as triosephosphate isomerase, phosphopyruvate hydratase, catalase, δ-glutathione S-transferase, ATP synthase alpha subunit and chymotrypsin. In contrast, the quantitative expression of 9 spots is decremented in the mated group which is identified as elongation factor 2, long-chain acetyl-CoA dehydrogenase, cryptocyanin 1, cryptocyanin 2, isocitrate dehydrogenase, aldehyde dehydrogenase X mitochondrial-like, transketolase, hemocyanin alpha-subunit and adenosyl-homocysteinase A. The results provide important theoretical support for completing ovary maturation research on the female Portunus trituberculatus. |
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| 备注/Memo: | 收稿日期: 2013-03-25. 基金项目: 国家自然科学基金（41106123）; 浙江省农业新品种选育重大专项（2012C12907-3）; 浙江省海水养殖重点科技创新团队项目（2010R50025-08）; 宁波市海洋与渔业专项（2011-1-7）; 浙江省教育厅科研项目（Z201121258）; 宁波市农业科技专项（201015）; 宁波市海洋蟹类产业科技创新团队项目（2011B81003）. 第一作者: 徐 洲（1989-）, 男, 江西九江人, 在读硕士研究生, 主要研究方向: 遗传育种与种质保护. E-mail: xuzhou9559@163.com *通信作者: 王春琳（1965-）, 男, 浙江台州人, 博导/教授, 主要研究方向: 水产养殖. E-mail: wangchunlin@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
