一种新型免疫传感器阵列检测癌胚抗原和糖类抗原199技术
PDF下载 (428)周 靖,胡富陶 *,余红卫.一种新型免疫传感器阵列检测癌胚抗原和糖类抗原199技术[J].宁波大学学报(理工版),2015,28(04):84-89.DOI:
ZHOU Jing,HU Fu-tao *,YU Hong-wei.A New Type of Sensor Array Detection of Carcinoembryonic Antigen and Carbohydrate Antigen 199 Technology[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(04):84-89.DOI:
| Title: | A New Type of Sensor Array Detection of Carcinoembryonic Antigen and Carbohydrate Antigen 199 Technology |
| 作者: | 周 靖, 胡富陶 *, 余红卫 |
| Author(s): | ZHOU Jing, HU Fu-tao *, YU Hong-wei |
| 关键词: | 分子印迹; 电化学发光; 免疫传感器; 疾病标志物 |
| Keywords: | molecular imprinting; electrochemiluminescent; immunosensor; disease markers |
| 分类号: | O657 |
| 文献标识码: | A |
| 摘要: | 制备了一种新型的含2个包覆分子印迹聚合物的丝网印刷碳工作电极的电化学发光夹心免疫传感器阵列, 通过该阵列实现了肿瘤标志物癌胚抗原(CEA)和糖类抗原199 (CA199)的近同时免疫分析. 该新型的电化学发光免疫方法将空间分辨技术与分子印迹技术相结合, 为CEA和CA199的复合免疫分析提供了一条简单、低成本、快速和灵敏的途径. 该方法对临床实验中多种蛋白质的近同时测定也显示了较大的潜力. |
| Abstract: | In this study, a novel electrochemiluminescence (ECL) immunosensor array is engineered consisting of two screen-printed carbon working electrodes coated with molecular imprinting polymer (MIPs). The array is formed to perform multiplexed immunoassay of tumor markers (carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (CA199)). Based on spatial-resolved technique, and coupled with molecularly imprinted technique, this novel ECL strategy provides a simple, low-cost, fast and sensitive approach for multiplexed immunoassay of CEA and CA199. This approach may render promising potential for protein detection in the circumstances of clinical laboratory setting. |
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| 备注/Memo: | 收稿日期: 2014-10-08. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省公益技术应用研究（2012C37012）; 宁波市自然科学基金（2013A610163）. 第一作者: 周靖（1989－）, 男, 湖南衡阳人, 在读硕士研究生, 主要研究方向: 分析化学. E-mail: zhouhuijie@nbu.edu.cn *通信作者: 胡富陶（1964－）, 男, 浙江磐安人, 高级实验师, 主要研究方向: 食品安全与检测. E-mail: E-mail: hufutao@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
