内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响
PDF下载 (429)周 飞1,2,张晓宏2,邹祖全2,张才乔1*.内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响[J].宁波大学学报(理工版),2015,28(03):98-102.DOI:
ZHOU Fei1,2,ZHANG Xiao-hong2,ZOU Zu-quan2,ZHANG Cai-qiao1*.Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(03):98-102.DOI:
| Title: | Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model |
| 作者: | 周 飞1, 2, 张晓宏2, 邹祖全2, 张才乔1* |
| Author(s): | ZHOU Fei1, 2, ZHANG Xiao-hong2, ZOU Zu-quan2, ZHANG Cai-qiao1* |
| 关键词: | fat-1转基因小鼠; 血糖; 血脂 |
| Keywords: | n-3 PUFAs; fat-1 transgenic mouse; n-3 polyunsaturated fatty acids; blood glucose; serum lipid |
| 分类号: | R151.2 |
| 文献标识码: | A |
| 摘要: | 利用fat-1转基因小鼠模型, 研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用. 采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周, 然后2组小鼠给予高糖饮液自由饮用4周, 每周测体重. 第8周末, 取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平. 结果表明: fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05). 认为n-3 PUFAs能抑制体重增长, 降低小鼠血糖、血脂和胰岛素抵抗, 起到调节血糖、血脂的作用. |
| Abstract: |
In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10% corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride (TG), serum total cholesterol (TC), serum high-density lipoprotein cholesterol (HDL-C), serum low-density lipoprotein cholesterol (LDL-C) levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TG, HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice (P<0.05). The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance. |
| 参考文献 /References: | [1].Manni A, Xu H, Washington S, et al. The impact of fish oil on the chemopreventive efficacy of tamoxifen against development of N-meth-yl-N-nitrosourea-induced rat mammary carcinogenesis[J]. Cancer Prev Res (Phila), 2010, 3(3):322-330. [2].Terry P D, Rohan T E, Wolk A. Intakes of fish and marine fatty acids and the risks of cancers of the breast and prostate and of other hor-mone-related cancer[J]. Am J Clin Nutr, 2003, 77(3):532. [3].La Rovere M T, Staszewsky L, Barlera S, et al. n-3 PUFA and Holter-derived autonomic variables in patients with heart failure: Data from the gruppo italiano per lo studio della sopravvivenza nell’ insufficienza cardiaca (GISSI- HF) Holter substudy[J]. Heart Rhythm, 2013, 10(2):226- 232. [4].Wu J H, Lemaitre R N, King I B, et al. Association of plasma phospholipid long-chain w-3 fatty acids with incident atrial fibrillation in older adults: the cardiovascular health study[J]. Circulation, 2012, 125(9): 1084-1093. [5].Phillipson B E, Rothrock D W, Connor W E, et al. Reduction of plasma lipids, lipoproteins, and apoproteins by dietary fish oils in patients with hypertrigly ceridemia [J]. N Engl J Med, 1985, 312(19):1210-1216. [6].Popp-Snijders C, Schouten J A, Heine R J, et al. Dietary supplementation of omega-3 polyunsaturated fatty acids improves insulin sensitivity in non-insulin-dependent diabetes[J]. Diabetes Res, 1987, 4(3):141-147. [7].Malasanos T H, Stacpoole P W. Biological effects of omega-3 fatty acids in diabetes mellitus[J]. Diabetes Care, 1991, 14(12):1160-1179. [8].Couet C, Delarue J, Ritz P, et al. Effect of dietary fish oil on body fat mass and basal fat oxidation in healthy adults [J]. Int J Obes Relat Metab Disord, 1997, 21(8):637-643. [9].Bucher H C, Hengstler P, Schindler C, et al. n-3 polyunsaturated fatty acids in coronary heart disease: A meta-analysis of randomized controlled trials[J]. Am J Med, 2002, 112(4):298-304. [10].Deckere E A. Possible beneficial effect of fish and fish n-3 polyunsaturated fatty acids in breast and colorectal cancer[J]. Eur J Cancer Prev, 1999, 8(3):213-221. [11].Sinn N, Bryan J. Effect of supplementation with polyunsaturated fatty acids and micronutrients on learning and behavior problems associated with child ADHD[J]. J Dev Behav Pediatr, 2007, 28(2):82-91. [12].Bousquet M, Saint-Pierre M, Julien C, et al. Beneficial effects of dietary omega-3 polyunsaturated fatty acid on toxin-induced neuronal degeneration in an animal model of Parkinson’s disease[J]. FASEB J, 2008, 22(4):1213- 1225. [13].Kang J X, Wang J, Wu L, et al. Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids[J]. Nature, 2004, 427: 504. [14].Duplus E, Glorian M, Tordjman J, et al. Evidence for selective induction of phosphoenolpyruvate carboxykinase gene expression by unsaturated and nonmetabolized fatty acids in adipocytes[J]. J Cell Biochem, 2002, 85(3): 651-661. [15].蒋立勤, 余陈欢, 余勤, 等. 两种海洋副产品的脂肪酸组成及其对高脂小鼠的影响[J]. 食品与机械, 2009(2): 58-61. [16].Buckley J D, Howe P R. Anti-obesity effects of long-chain omega-3 polyunsaturated fatty acids[J]. Obese Rev, 2009, 10(6):648-659. [17].Wang H, Storlien L H, Huang X F. Effects of dietary fat types on body fatness, leptin, and ARC leptin receptor NPY, and AgRP mRNA expression[J]. Am J Physiol Endocrinol Metab, 2002, 282(6):1352-1359. [18].Baillie R A, Takada R, Nakamura M, et al. Coordinate induction of peroxisomal-acyl-CoA oxidase and UCP-3 by dietary fish oil: A mechanism for decreased body fat deposition[J]. Prostaglandins Leukot Essent Fatty Acids, 1999, 60(5/6):351-356. [19].Hill J O, Peters J C, Lin D, et al. Lip accumulation and body fat distribution is influenced by type of dietary fat fed to rats[J]. Int J Obes Relat Metab Disord, 1993, 17(4): 223-236. [20].Newman R E, Bryden W L, Fleck E, et al. Dietary n-3 and n-6 fatty acids alter avian metabolism: Metabolism and abdominal fat deposition[J]. Br J Nutr, 2002, 88(1): 11-18. [21].Todoric J, Loffler M, Huber J, et al. Adipose tissue inflammation induced by high fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids[J]. Diabetologia, 2006, 49(9):2109-2119. [22].Nettleton J A, Katz R. n-3 long-chain polyunsaturated fatty acids in type 2 diabetes: a review[J]. Am Diet Assoc, 2005, 105(3):428-440. [23].赵冬. 中国人群的血脂流行病学研究[J]. 中华心血管病杂志, 2003, 31:74-78. [24].陆宗良. 血脂异常与心血管病危险因素控制[J]. 中国心血管杂志, 2001, 29:315-317. [25].Theis F, Garry J M, Yaqoob P, et al. Association of n-3 polyunsaturated fatty acids with stability of athero- sclerotic plaques: A randomised controlled trial[J]. Lancet, 2003, 361:477-485. |
| 备注/Memo: | 收稿日期: 2014?05?31. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金青年科学基金（81102121）. 第一作者: 周飞（1981－）, 男, 安徽濉溪人, 助理实验师, 主要研究方向: 实验动物学. E-mail: zhoufei@nbu.edu.cn *通信作者: 张才乔（1965－）, 男, 浙江宁波人, 教授, 主要研究方向: 基础兽医学. E-mail: cqzhang@zju.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
