不同氮磷质量浓度下青岛大扁藻和牟氏角毛藻的种间竞争关系
PDF下载 (579)林忠洲,徐善良,邵 波,周 伟,严小军.不同氮磷质量浓度下青岛大扁藻和牟氏角毛藻的种间竞争关系[J].宁波大学学报(理工版),2013,26(01):12-17.DOI:
LIN Zhong-zhou,XU Shan-liang,SHAO Bo,ZHOU Wei,YAN Xiao-jun.The Competitive Relationship of Platymonas helgolandica Var. Tsingtaoensis and Chaetoceros Muelleri Lemmerman in Different Mass Concentrations of Nitrogen and Phosphorus[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,26(01):12-17.DOI:
| Title: | The Competitive Relationship of Platymonas helgolandica Var. Tsingtaoensis and Chaetoceros Muelleri Lemmerman in Different Mass Concentrations of Nitrogen and Phosphorus |
| 作者: | 林忠洲, 徐善良, 邵 波, 周 伟, 严小军 |
| Author(s): | LIN Zhong-zhou, XU Shan-liang, SHAO Bo, ZHOU Wei, YAN Xiao-jun |
| 关键词: | 青岛大扁藻; 牟氏角毛藻; 氮磷; 共培养; 种间竞争 |
| Keywords: | sub-critical extraction; dimethyl ether; scombermorus; fish oil |
| 分类号: | Q948.8 |
| 文献标识码: | A |
| 摘要: | 为研究青岛大扁藻(Platymonas helgolandica var. Tsingtaoensis)和牟氏角毛藻(Chaetoceros muelleri Lemmerman)的种间竞争关系, 以KNO3和KH2PO4为N源和P源, 各设5个质量浓度梯度, 观察了2种微藻单种培养与共培养时的生长特性. 结果表明: (1)单种培养时, 青岛大扁藻密度与氮、磷质量浓度正相关(P<0.05); 牟氏角毛藻密度与氮质量浓度正相关, 但磷以1.14 mg?L-1组(P3)藻密度最高. (2)共培养时, 2种藻的生长效果均不如单种培养, 存在显著差异(P<0.05). (3)青岛大扁藻最适宜N/P比值为14.81, 对磷有较高的需求; 而牟氏角毛藻的最适宜N/P比值是26.93, 其对较低的磷浓度具有更好的适应性. (4)共培养下, 氮处理组青岛大扁藻占较大竞争优势, 磷处理组牟氏角毛藻占更多竞争优势; 在培养早期和末期青岛大扁藻具有明显的竞争优势, 而在培养中期牟氏角毛藻竞争优势更大. |
| Abstract: | In this study, the efficacy of subcritical extraction techniques is investigated for extracting fish oil from by-products of Cybium Niphonium. In our experiment, dimethyl ether (DME) was used as an extracting medium, the extracting pressure was set up at 0.8-1.0MPa. Taking the extraction yield of Cybium Niphonium oil as the examination target, the factors influencing fish oil extraction were observed and recorded. First, discussions are made on the extract time, the extract temperature, and the extract times, all of which are based on the single factor experiment to withdraw the effect the influence. Then an orthogonal experimental design was used, in which the fish oil yield is used as a dependent variable, and the independent variables include extracting time, extracting temperature and extracting times. The results show that the optimal extracting conditions for maximum oil yield are as follows: 45min extracting time, 40℃ extracting temperature, and 3 extracting times, which generates the oil yield of 73.52%. This study suggests that application of subcritical extraction technology in extraction of fish oil from Cybium Niphonium is an effective and economic approach for producing high quality fish oil at low cost. |
| 参考文献 /References: | [1] 刘平怀, 时杰, 沈世军. 亚临界萃取法回收废弃虾蟹壳中虾青素[J]. 精细化工, 2011, 28(5):497-500. [2] Mustapa A, Manan Z, Mohd Azizi C, et al. Extraction of β-carotenes from palm oil mesocarp using sub-critical R134a[J]. Food Chemistry, 2011, 125:262-267. [3] 林承杰, 张力, 刘明金, 等. 玫瑰精油真空水散冲蒸亚临界流体萃取技术: 中国, 101096614[P]. 2008-01-02. [4] 李超, 郑义, 王卫东, 等. 响应曲面法优化亚临界水提取葡萄籽原花青素的工艺研究[J]. 食品科学, 2010, 31(12):6-10. [5] 刘玉梅, 刘奎钫, 石波, 等. 一种生产番茄红素油树脂的方法: 中国, 101928473[P]. 2010-12-29. [6] 何蓉蓉, 张延延, 曹云峰, 等. 五味子提取物及其制备方法和应用: 中国, 101978990[P]. 2011-02-23. [7] 林长华. 漫话马鲛鱼[J]. 中国水产, 2011(3):78-79. [8] 黄克勤. 马鲛鱼[J]. 水产科技情报, 1973(11):31. [9] 娄永江, 杨文鸽, 王扬, 等. 马鲛鱼方便食品的开发[J]. 食品科技, 2000(6):31. [10] 李西腾. 马鲛鱼丸的加工工艺研究[J]. 农业科技与装备, 2011(7):26-29. |
| 备注/Memo: | 收稿日期: 2012?10?10. 基金项目: 国家海洋公益科研专项（201105009-3）; 浙江省“新农村科技示范点建设”项目（XJKS2010001）; 宁波市重大攻关项目（2011C11003）.第一作者: 林忠洲（1987－）, 男, 浙江绍兴人, 在读硕士研究生, 主要研究方向: 海洋生态学研究. E-mail: linzhongzhou@gmail.com. *通信作者: 徐善良（1962－）, 男, 浙江舟山人, 副研究员, 主要研究方向: 海水养殖技术. E-mail: xushanliang@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
