新型镁合金螺钉体外腐蚀降解及细胞活性研究
PDF下载 (250)杨 越,张雁儒,徐景超,李 昊,李洁洁,余进伟.新型镁合金螺钉体外腐蚀降解及细胞活性研究[J].宁波大学学报(理工版),2022,35(1):33-39.DOI:
YANG Yue,ZHANG Yanru,XU Jingchao,LI Hao,LI Jiejie,YU Jinwei.In vitro corrosion degradation and cell activity analysis of new magnesium alloy screws[J].Journal of Ningbo University(Natural Science & Engineering Edition),2022,35(1):33-39.DOI:
| Title: | In vitro corrosion degradation and cell activity analysis of new magnesium alloy screws |
| 作者: | 杨 越, 张雁儒, 徐景超, 李 昊, 李洁洁, 余进伟 |
| Author(s): | YANG Yue, ZHANG Yanru, XU Jingchao, LI Hao, LI Jiejie, YU Jinwei |
| 关键词: | 新型镁合金; 腐蚀电位; 体外降解; 细胞活性 |
| Keywords: | new magnesium alloy; corrosion potential; in vitro degradation; cell activity |
| 分类号: | R608 |
| 文献标识码: | A |
| 摘要: | 研究了基于NZ30K合金开发的新型Mg-3Nd-0.2Zn-0.4Zr-0.2Mn镁合金耐腐蚀性、体外降解行为特性及浸提液细胞生物毒性. 采用金相显微镜得到新型镁合金金相显微图, 采用扫描电镜(Scanning Electron Microscope, SEM)获取SEM图; 采用武汉科思特电化学工作站进行电化学测试, 并绘制动电位极化曲线, 以磷酸盐缓冲液(Phosphate Buffer Solution, PBS)模拟体液环境, 记录氢气析出体积并计算腐蚀速率; 利用细胞完全培养基测定pH值、重量变化曲线; 获取大鼠骨髓间充质干细胞(Bone Mesenchymal Stem Cells, BMSCs), 并利用完全细胞培养基制作新型镁合金浸提液, 检测细胞生物活性, 以ZA75镁合金为基础添加0.3%Mn元素制成合金作为对照组, 比较腐蚀电位、体外降解情况以及细胞活性. 结果表明: 新型Mg-3Nd-0.2Zn-0.4Zr-0.2Mn镁合金横截面等轴晶体组织细小均匀性较好, 纵截面呈长条状组织均匀性稍差; 自腐蚀电位较高, 为-1.3912V; 自腐蚀电流密度较低, 为7.37×10-7 A?cm-2; 体外析氢量低, 失重量、pH值变化幅度相对较小; 降解速率下降后呈现小范围上升后趋于平缓; 具有良好的细胞相容性, 可以促进BMSCs细胞增殖分化. |
| Abstract: | The current study was designed to investigate the corrosion resistance, in vitro degradation behavior and cell biotoxicity of new Mg-3ND-0.2Zn-0.4Zr-0.2Mn magnesium alloy based on NZ30K alloy. The metallographic images of the new magnesium alloy were obtained by metallographic microscope, and the SEM images were obtained by Scanning Electron Microscope. Electrochemical tests were carried out by using Wuhan Coster Electrochemical Workstation, with which the potentiodynamic polarization curves were drawn. Hydrogen precipitation volume was recorded and corrosion rate was calculated using Phosphate Buffer Solution (PBS) to simulate the body fluid environment. Both the value of pH and weight curve were measured by cell complete medium. Rat Bone Marrow Mesenchymal Stem Cells (BMSCs) were obtained, and a new magnesium alloy extract was prepared using complete cell culture medium to detect the biological activity of the cells. The corrosion potential, in vitro degradation and cell activity of ZA75 magnesium alloy were compared with 0.3% Mn alloy as control group. The results show that the new Mg-3Nd-0.2Zn-0.4Zr-0.2Mn magnesium alloy has finer and more uniform microstructure in equiaxed cross section, and lower uniformity in long strip structure in longitudinal section. The higher self-corrosion potential is -1.3912V, and the lower self-corrosion current density is 7.37×10-7 A?cm-2. In vitro hydrogen evolution is lower, and weight loss and pH value variation are relatively small. Degradation rate decreased and subsequently increased in a small range. The trend tends to be mild. It has good cytocompatibility and can promote the proliferation differentiation of BMSCs cells. The new Mg-3Nd-0.2Zn-0.4Zr-0.2Mn magnesium alloy has greater corrosion resistance, stable degradation rate and good cellular compatibility. It has a broad prospect in practical application. |
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| 备注/Memo: | 收稿日期: 2021－10－27. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 河南省科技攻关重点项目（201402003）; 2017年河南省高等学校重点科研项目（13170023）. 第一作者: 杨越（1998－）, 男, 河南信阳人, 在读硕士研究生, 主要研究方向: 骨科植入材料. E-mail: CAS1908@126.com *通信作者: 张雁儒（1970－）, 男, 河南西华人, 教授, 主要研究方向: 创伤骨科. E-mail: zyr@hpu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
