日本血吸虫病肉芽肿的电镜观察
PDF下载 (434)蒋雯雯,胡奇丰,柳建发 *.日本血吸虫病肉芽肿的电镜观察[J].宁波大学学报(理工版),2013,26(02):130-133.DOI:
JIANG Wen-wen,HU Qi-feng,LIU Jian-fa *.Electronic Transmissive Microscope Observation on Hepatic Egg Granuloma of Schistosomiasis japonicum in Gerbil Mice[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,26(02):130-133.DOI:
| Title: | Electronic Transmissive Microscope Observation on Hepatic Egg Granuloma of Schistosomiasis japonicum in Gerbil Mice |
| 作者: | 蒋雯雯, 胡奇丰, 柳建发 * |
| Author(s): | JIANG Wen-wen, HU Qi-feng, LIU Jian-fa * |
| 关键词: | 日本血吸虫病; 肝脏; 虫卵肉芽肿; 透射电镜 |
| Keywords: | Schistosomiasis japonicum; liver; egg granuloma; TEM |
| 分类号: | R383.2+4 |
| 文献标识码: | A |
| 摘要: | 应用长爪沙鼠建立日本血吸虫病动物模型, 观察实验性日本血吸虫病肝脏肉芽肿的超微结构改变. 采用腹部贴片法感染血吸虫尾蚴, 每只沙鼠感染(120±2)条尾蚴, 于5周后解剖, 取出肝脏做病理组织学和透射电镜观察. 结果发现, 肝脏切片H.E.(铁苏木素伊红)染色后可观察到虫卵肉芽肿产生, 透射电镜观察, 可见超微结构改变. 肝脏肉芽肿的超微结构的病变可以清晰地显示肉芽肿的发生机制, 为实验性研究血吸虫病的防治奠定了基础. |
| Abstract: | To observe the ultrastructural changes of hepatic egg granuloma of experimental Schistosomiasis japonicum, the gerbil mice model of Schistosomiasis is used. The mice have been infected individually with cercariae of Schistosoma japonicum (120±2), all of which were autopsied at the fifth week after infection. The liver was sectioned and observed by HE staining and electronic transmissive microscope. Results show that the nodules on the surface of liver and egg granuloma have been observed, and intact egg and ultrastructural changes have been noted. This research establishes the gerbil model of Schistosomiasis, and the liver egg granuloma is investigated. The results are expected to add some helpful references for the prevention of Schistosomiasis. |
| 参考文献 /References: | [1]Norman L, Kagan IG. The maintenance of echinococcus multilocularis in gerbils (meriones unguiculatus) by intraperitoneal inoculation[J]. J Parasitol, 1961, 47:870- 874. [2]柳建发, 廖奇. 血吸虫病疫苗研究的回顾与展望[J]. 宁波大学学报: 理工版, 2012, 25(4):111-114. [3]杨瑾, 姚锦蕾, 柳建发. 日本血吸虫病动物模型的研究进展[J]. 地方病通报, 2009, 24(2):69-71. [4]柳建发, 蒋雯雯, 胡奇丰. 早期日本血吸虫感染长爪沙鼠动物模型的建立[J]. 宁波大学学报: 理工版, 2011, 24(1):95-96. [5]柳建发, 蒋雯雯, 周飞. 长爪沙鼠寄生虫感染和疾病动物模型的建立[J]. 宁波大学学报: 理工版, 2010, 23(4): 117-120 [6]蒋雯雯, 胡奇丰, 柳建发. 长爪沙鼠日本血吸虫病动物模型的建立[J]. 宁波大学学报: 理工版, 2011, 24(2): 107-109. [7]Bergquist N R, Colley D G. Schistosomiasis vaccines: Research to development[J]. Parasitology Today, 1998, 14(3):99-104. [8]Mauricio G Y. Antihelminthic and antiparasitic drugs: Unapproved uses or indications[J]. Clinics in Dermato- logy, 2000, 18:113-117. [9]Xiao Shuhua, Marcel T, Shen Binggui, et al. Effect of artemether on the tegument of adult schistosoma haematobium recorved from mice[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(6):425-432. |
| 备注/Memo: | 收稿日期: 2012－11－06. 基金项目: 宁波市自然科学基金（2010A610036）. 第一作者: 蒋雯雯（1969－）, 女, 江西南昌人, 实验师, 主要研究方向: 病原生物学. E-mail: jiangwenwen@nbu.edu.cn *通信作者: 柳建发（1964－）, 男, 江西九江人, 副教授, 主要研究方向: 病原生物学. E-mail: liujianfa@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
