大黄鱼肝脏的显微及超微结构
PDF下载 (581)周 阳1,王友发1,沈伟良2,王建平2,吴雄飞2,竺俊全1*.大黄鱼肝脏的显微及超微结构[J].宁波大学学报(理工版),2017,30(1):42-46.DOI:
ZHOU Yang1,WANG You-fa1,SHEN Wei-liang2,WANG Jian-ping2,WU Xiong-fei2,ZHU Jun-quan1*.Microstructures and ultrastructures of liver in Larimichthys crocea[J].Journal of Ningbo University(Natural Science & Engineering Edition),2017,30(1):42-46.DOI:
| Title: | Microstructures and ultrastructures of liver in Larimichthys crocea |
| 作者: | 周 阳1, 王友发1, 沈伟良2, 王建平2, 吴雄飞2, 竺俊全1* |
| Author(s): | ZHOU Yang1, WANG You-fa1, SHEN Wei-liang2, WANG Jian-ping2, WU Xiong-fei2, ZHU Jun-quan1* |
| 关键词: | 大黄鱼; 肝脏; 组织学; 超微结构 |
| Keywords: | Larimichthys crocea; liver; histology; ultrastructure |
| 分类号: | Q954.6 |
| 文献标识码: | A |
| 摘要: | 为了解大黄鱼肝脏的结构特点, 采用石蜡切片-显微技术及超薄切片-透射电镜技术观察研究了健康大黄鱼肝脏的组织学结构特征. 显微观察显示, 肝脏表面覆有一层较薄的结缔组织被膜, 肝小叶之间界限不明显, 中央静脉不发达, 肝细胞索(板)排列不规则; 肝血窦相互吻合成网络状; 肝内小叶间静脉、小叶间动脉伴行; 肝内胆管系统由胆小管、小叶内胆管、小叶间胆管和支胆管组成; 小叶间胆管常与小叶间静脉、小叶间动脉伴行; 胰腺组织弥散分布于肝实质中. 电镜观察显示, 大黄鱼肝细胞界限清晰, 单核; 细胞质分布有丰富的核糖体和线粒体, 内质网发达, 含有丰富的糖原颗粒和大小不等的脂滴; 枯否氏细胞具伪足, 细胞质内含有吞噬泡; 贮脂细胞内含有大小不等的脂滴; 狄氏隙及胆小管腔内充满丰富的微绒毛. 大黄鱼肝脏组织结构与其他硬骨鱼类基本相似. |
| Abstract: | In order to study the structure of liver in large yellow croaker (Larimichthys crocea), we use the paraffin section and transmission electronmicroscope to observe the structure of L. crocea liver. The light microscope demonstrates that the surface of liver is covered with a thin layer of connective tissues capsule. The boundaries between the hepatic lobule are not clearly noticeable. Central vein located in the center of hepatic lobule is not developed. The pattern of hepatic cords is irregularly arranged. The hepatic sinusoids form the webs of honey comb, and the interlobular vein and interlobular artery always appear together. The bile duct system of liver consists of the bile canaliculi, intralobular biliary canals, interlobular bile duct and branched bile duct. The portal area is composed of the interlobular bile duct, interlobular vein and interlobular artery. The pancreas contained is distributed randomly in the liver parenchyma. Electron microscope shows that the boundaries of liver cell look clear. Liver cells are with single nucleus. The cytoplasm of liver cell has a large number of organelles (mitochondria, ribosome, endoplasmicreticulum) and abundant inclusions (glycogenosome and lipiddroplet). The kupffer cells which have pseudopod and phagosome, and hepatic stellate cells varying in size of lipiddroplet are found in the hepatic sinusoids. The hepatic sinusoids and bile canaliculi are full of large amount of microvilli. It is also noticed that, in general, the structure of L. crocea liver is similar with that of teleost fishes |
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| 备注/Memo: | 收稿日期: 2016?01?04. 宁波大学学报(理工版)网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市科技计划重大项目(2015C110005); 浙江省科技计划项目(2012C12907-8); 宁波市自然科学基金(2013A610165). 第一作者: 周阳(1989-), 女, 黑龙江佳木斯人, 在读硕士研究生, 主要研究方向: 鱼类繁殖与发育. E-mail: 15825572309@163.com *通信作者: 竺俊全(1964-), 男, 浙江宁波人, 教授, 主要研究方向: 鱼类繁殖与发育. E-mail: zhujunquan@nbu.edu.cn 宁波大学学报(理工版)网址:http://journallg.nbu.edu.cn/ |