虎斑乌贼精子发生及精子超微结构
PDF下载 (401)陈奇成,蒋霞敏,韩庆喜,彭瑞冰,江茂旺,韩庆功.虎斑乌贼精子发生及精子超微结构[J].宁波大学学报(理工版),2019,32(2):1-8.DOI:
CHEN Qi-cheng,JIANG Xia-min,HAN Qing-xi,PENG Rui-bing,JIANG Mao-wang,HAN Qing-gong.Spermatogenesis and sperm ultrastructure of Sepia pharaonis[J].Journal of Ningbo University(Natural Science & Engineering Edition),2019,32(2):1-8.DOI:
| Title: | Spermatogenesis and sperm ultrastructure of Sepia pharaonis |
| 作者: | 陈奇成, 蒋霞敏, 韩庆喜, 彭瑞冰, 江茂旺, 韩庆功 |
| Author(s): | CHEN Qi-cheng, JIANG Xia-min, HAN Qing-xi, PENG Rui-bing, JIANG Mao-wang, HAN Qing-gong |
| 关键词: | 虎斑乌贼; 精子发生; 超微结构 |
| Keywords: | Sepia pharaonis; spermatogenesis; ultrastructure |
| 分类号: | Q954.2 |
| 文献标识码: | A |
| 摘要: | 为了丰富虎斑乌贼(Sepia pharaonis)繁殖生物学资料, 采用扫描透射电镜技术, 观察了虎斑乌贼精子发生及精子的超微结构特征. 结果显示: 虎斑乌贼的精子发生过程可分为精原细胞、初级精母细胞、次级精母细胞、精细胞、成熟精子5个阶段. 在精子形成期间, 细胞核横向收缩纵向拉长, 最终变为圆柱形, 核后窝偏位; 染色质由絮状、均匀分散状、点状、细纤维状、粗纤维状, 最后形成高电子密度的均质状; 线粒体形状由空泡状变为多嵴的椭球状, 位置由顶体囊周围移动至核后端, 最后聚集在鞭毛一侧形成线粒体距. 精子长约为93.00μm, 可分为头部和尾部. 头部呈长辣椒状, 包括顶体和细胞核, 长7.64μm; 尾部可分为线粒体距和鞭毛两部分, 鞭毛长86.30μm, 主段被9束粗纤维包围形成典型“9+9+2”结构. |
| Abstract: | In order to explore the reproductive biology and provide theoretical guidance for the artificial breeding of Sepia pharaonis, the sperm ultrastructure of Sepia pharaonis and the changes of organelles during spermatogenesis were examined by scanning electron microscopy and transmission electron microscopy. There are five developmental stages of spermatogenesis in Sepia pharaonis: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and mature spermatozoon. The nucleus underwent a longitudinal elongation of lateral contraction and eventually became cylindrical. The posterior nuclear fossa diverged from the center of the nucleus. Chromatin in the nucleus underwent flocculation, homogenization, density of fine particles, fine fibers and coarse fibers, eventually condensing into regions of high electron density. The shape of mitochondria changed from a vacuole to a spheroidal shape. Mitochondria moved from the periphery of the acrosomal vesicle to the nucleus of the nucleus, and finally gathered on the side of the flagella to form a mitochondrial spur. The spermatozoon was 93.00 μm and consisted of the head and the tail. The head that looked like a long pepper was 7.64 μm and consisted of acrosome and nucleus. The tail can be divided into mitochondrial spur and flagella. The flagellum was 86.30 μm and surrounded by nine bundles of coarse fibers forming a “9+9+2” array. |
| 参考文献 /References: | [1] 焦海峰, 尤仲杰, 竺俊全. 软体动物精子的超微结构及其分类学意义[J]. 宁波大学学报(理工版), 2004, 17(4): 414-418. [2] 竺俊全, 杨万喜, 尤仲杰, 等. 嘉庚蛸精子发生的超微结构[J]. 水产学报, 2006, 30(2):161-169. [3] 叶素兰, 吴常文, 余治平. 曼氏无针乌贼(Sepiella maindroni)精子形成的超微结构[J]. 海洋与湖沼, 2008, 39(3):269-275. [4] 刘长琳, 邹健, 刘君刚, 等. 金乌贼精子的超微结构[J]. 渔业科学进展, 2011, 32(3):1-6. [5] 罗江, 蒋霞敏, 唐锋, 等. 拟目乌贼精子发生和精子的超微结构[J]. 动物学杂志, 2014, 49(1):71-82. [6] 李来国. 长蛸生殖系统组织学和精卵发生的细胞学研究[D]. 宁波: 宁波大学, 2010. [7] Mann T, Martin A W, Thiersch J B. Male reproductive tract, spermatophores and spermatophoric reaction in the giant octopus of the North Pacific, Octopus dofleini martini[J]. Nature, 1966, 211(5055):1279-1282. [8] Maxwell W L. Spermiogenesis of Eledone cirrhosa Lamarck (Cephalopoda, Octopoda)[J]. Proceedings of the Royal Society of London, 1974, 186(1083):181-190. [9] Maxwell W L. Spermiogenesis of Eusepia officinalis (L.), Loligo forbesi (Steenstrup) and Alloteuthis subulata (L.) (Cephalopoda, Decapoda)[J]. Proceedings of the Royal Society of London, 1975, 191(1105):527-535. [10] Fields W G, Thompson K A. Ultrastructure and functional morphology of spermatozoa of Rossia paci[J]. Canadian Journal of Zoology, 2011, 54(6):908-932. [11] Healy J M. Ultrastructure of spermiogenesis in Vampyro- teuthis infernalis Chun: A relict cephalopod mollusk[J]. Helgol?nder Meeresuntersuchungen, 1990, 44(1):95-107. [12] Martínezsoler F, Kurtz K, Chiva M. Sperm nucleo- morphogenesis in the cephalopod Sepia officinalis[J]. Tissue & Cell, 2007, 39(2):99-108. [13] Ribes E, Giménezbonafé P, Martínezsoler F, et al. Chromatin organization during spermiogenesis in Octopus vulgaris I: Morphological structures[J]. Molecular Reproduction & Development, 2004, 68(2):223-231. [14] Healy J M. Ultrastructure of spermatozoa and spermio- genesis in Spirula spirula (L.): Systematic importance and comparison with other cephalopods[J]. Helgol?nder Meeresuntersuchungen, 1990, 44(1):109-123. [15] 陈新军, 刘必林, 王尧耕. 世界头足类[M]. 北京: 海洋出版社, 2009:1-699. [16] 高晓兰, 蒋霞敏, 乐可鑫, 等. 野生虎斑乌贼不同组织营养成分分析及评价[J]. 动物营养学报, 2014, 26(12): 3858-3867. [17] 黄建盛, 陈刚, 张健东, 等. 野生虎斑乌贼(Sepia pharaonis)肌肉主要营养成分分析及评价[J]. 营养学报, 2014, 36(5):502-504. [18] 陈道海, 文菁, 赵玉燕, 等. 野生与人工养殖的虎斑乌贼肌肉营养成分比较[J]. 食品科学, 2014, 35(7):217-222. [19] Thanonkaew A, Benjakul S, Visessanguan W. Chemical composition and thermal property of cuttlefish (Sepia pharaonis) muscle[J]. Journal of Food Composition & Analysis, 2006, 19(2):127-133. [20] Minton J W. The pattern of growth in the early lifecycle of individual Sepia pharaonis[J]. Marine & Freshwater Research, 2004, 55(4):415-422. [21] 蒋霞敏, 罗江, 彭瑞冰, 等. 水泥池养殖条件下虎斑乌贼的生长特性[J]. 宁波大学学报(理工版), 2014, 27(2):1-6. [22] 陈道海, 王雁, 梁汉青, 等. 虎斑乌贼(Sepia pharaonis)胚胎发育及孵化历期观察[J]. 海洋与湖沼, 2012, 43(2): 394-400. [23] 陈道海, 郑亚龙. 虎斑乌贼(Sepia pharaonis)繁殖行为谱分析[J]. 海洋与湖沼, 2013, 44(4):931-936. [24] 彭瑞冰, 乐可鑫, 蒋霞敏, 等. 虎斑乌贼受精卵卵黄营养成分分析[J]. 水产学报, 2015, 39(7):1034-1042. [25] 阮鹏, 蒋霞敏, 韩庆喜, 等. 社会等级因素对虎斑乌贼生长、存活及相关酶活的影响[J]. 水产学报, 2016, 40(12):1897-1905. [26] 王双健, 丁玉惠, 周爽男, 等. 虎斑乌贼喷墨卵与正常卵的比较[J]. 水产学报, 2017, 41(3):366-373. [27] Fawcett D W, Anderson W A, Phillips D M. Morpho- genetic factors influencing the shape of the sperm head[J]. Developmental Biology, 1971, 26(2):220-251. |
| 备注/Memo: | 收稿日期: 2018－10－22. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（41806186）; 宁波市重大专项（201401C1111001）; 宁波市富民项目（2017C10006）. 第一作者: 陈奇成（1994－）, 男, 浙江台州人, 在读硕士研究生, 主要研究方向: 海洋生物育种与健康养殖. E-mail: 924954174@qq.com *通信作者: 蒋霞敏（1957－）, 女, 浙江宁波人, 教授, 主要研究方向: 水产动物繁育. E-mail: jiangxiamin@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
