免疫多糖对日本蟳溶菌酶和超氧化物歧化酶活性的影响
PDF下载 (480)周 伟,徐善良,石 灏.免疫多糖对日本蟳溶菌酶和超氧化物歧化酶活性的影响[J].宁波大学学报(理工版),2013,26(03):17-21.DOI:
ZHOU Wei,XU Shan-liang,SHI Hao.Effects of Immunopolysacchride on LZM and SOD Activity of Charybdis japonicas[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,26(03):17-21.DOI:
| Title: | Effects of Immunopolysacchride on LZM and SOD Activity of Charybdis japonicas |
| 作者: | 周 伟, 徐善良, 石 灏 |
| Author(s): | ZHOU Wei, XU Shan-liang, SHI Hao |
| 关键词: | 日本蟳; 免疫多糖; 溶菌酶; 超氧化物歧化酶; 免疫增强 |
| Keywords: | Charybdis japonica; immunopolysacchride; LZM; SOD; immunoenhancement |
| 分类号: | Q956 |
| 文献标识码: | A |
| 摘要: | 通过对日本 (Charybdis japonica)进行免疫多糖、免疫多糖+溶藻弧菌、溶藻弧菌、生理盐水4种方法的处理, 测定日本 肝胰腺及肌肉中溶菌酶(LZM)和超氧化物歧化酶(SOD)的活性变化, 探讨免疫多糖对日本 溶菌酶和超氧化物歧化酶活性的影响. 结果表明: 日本 肝胰腺中LZM和SOD的酶活均高于肌肉中的酶活, 且免疫多糖对肝胰腺的免疫增强效应显著高于肌肉; 免疫多糖组肝胰腺和肌肉中LZM酶活, 在注射后24h时显著升高至峰值(P<0.05), 分别为(11.2 ± 2.78)U?mg-1和(3.75±1.05) U?mg-1; SOD酶活也在24h达到最高值, 分别是对照组的2.2倍和1.4倍; 免疫多糖+溶藻弧菌组在72h时, 肝胰腺和肌肉中的LZM酶活分别高出溶藻弧菌组62.8%和75.2%, 2个组织中SOD酶活同样显著高于溶藻弧菌组(P<0.05), 是溶藻弧菌组的3.5倍和4.2倍. 由此可见, 免疫多糖对日本 免疫功能有增强作用, 可以提高日本 的抗病能力. |
| Abstract: | The Charybdis japonicas was performed with different treatments: normal saline controled group, Vibrio alginolyticus infected group, immunopolysaccharide group, immunopolysacchride and after Vibrio alginolyticus infected group. Then we analysed the changes of the lysozyme (LZM) and superoxide dismutase (SOD) in the hepatopancreas and muscle. The results showed that the activities of LZM and SOD in hepatopancreas were higher than the muscles’. The effect of immunoenhancement on the hepatopancreas was also significantly higher than muscle’s. Besides, LZM’s activity of the immune group was elevated to the peak (P<0.05) after 24 hours in the hepatopancreas and muscle,which was (11.2±2.78) U?mg-1 and (3.75±1.05) U?mg-1. And SOD’s activity also reached the peak after 24 hours, which was 2.2 and 1.4 times of control group in the hepatopancreas and muscle. After 72 hours LZM’s activities of the immuned and bacterial infected group were 62.8% and 75.2% higher than the bacterial infected group in the hepatopancreas and muscle. The SOD’s activities were also significantly higher than bacterial infected group (P<0.05), which were 3.5 and 4.2 times of the bacterial infected group. Therefore, immunopolysacchride maked a effective immune enhancement of Charybdis japonica and improved the resistance ability of disease. |
| 参考文献 /References: | [1] 管晓娟. 甲壳动物体液免疫相关酶及免疫因子研究概况[J]. 生命科学仪器, 2009, 7(4):3-7. [2] 陈昌福, 陈萱, 陈超然, 等. 水产甲壳动物的免疫防御机能及其免疫预防研究进展[J]. 华中农业大学学报, 2003, 22(2):197-203. [3] 李花霞, 杨文鸽. 甲壳动物免疫因子研究的进展[J]. 水生生物学报, 2003, 27(4):418-420. [4] Rosario C, Norma C, Alex O. Effect of different β- glucans on t he respiratory burst of turbot (Psetta maxima) and gilt head sea bream (Sparus aurata) phagocytes[J]. Fish & Shellfish Immunology, 1999, 9(7):529-541. [5] Luo Zhang, Yao Juan, Chen Changfu, et al. Infuluence of dietary yeast immunopolysacchride on the resistance of channel catfish (Ictalurus punctatus) against Aeromonas hydrophila[J]. Freshwater Fisheries, 2007(3):287-297. [6] 胡火庚, 陈昌福. 饲料中添加酵母复合物对中华绒螯蟹的免疫调节作用[J]. 长江大学学报: 农学卷, 2007, 4(2):36-40. [7] 沈文英, 阳会军, 柯慧芬, 等. β-葡聚糖对凡纳滨对虾免疫相关酶活性的影响[J]. 水产科学, 2007, 26(7): 381-383. [8] Suphantharika M, Khunrae P, Thanardkit P, et al. Preparation of spent brewer’s yeast β-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon[J]. BioreSource Technology, 2003, 88:55-60. [9] 陈昌福, 姚鹃, 陈萱, 等. 口服免疫多糖(酵母细胞壁)对南美白对虾抗哈维弧菌感染的作用[J]. 安徽农业大学学报, 2004, 31(4):402-405. [10] 徐镇, 姚娟, 陈昌福, 等. 免疫多糖(酵母细胞壁)对中华绒螯蟹抗病力的增强效果[J]. 华中农业大学学报, 2005, 24(4):383-386. [11] 许第新, 姚娟, 陈昌福. 注射免疫多糖(酵母细胞壁)对克氏原螯虾几种免疫相关酶活性的影响[J]. 淡水渔业, 2004, 34(5):56-58. [12] 王洪斌, 李士虎, 阎斌伦, 等. 海洋红酵母多糖提取及其对日本蟳血清中部分免疫活性因子的影响[J]. 微生物学通报, 2011, 38(2):176-180. [13] 仲伟静, 王安利. 对虾免疫多糖的研究进展[J]. 水产科学, 2009, 28(8):482-483. [14] 谭夕东, 李义, 张红英, 等. 海藻酸钠对中华绒螯蟹免疫功能及抗病力的影响[J]. 饲料工业, 2008, 29(6):24- 27. [15] 沈锦玉, 刘问, 曹铮, 等. 免疫增强剂对中华绒螯蟹免疫功能的影响[J]. 浙江农业学报, 2004, 16(1):25-29. [16] 余水法, 蔡春芳, 宋学宏, 等. β-葡聚糖对河蟹免疫功能的影响[J]. 中国饲料, 2006, 5:20-22. [17] 黄旭雄, 周洪琪, 宋理平. 急性感染对中国明对虾非特异免疫水平的影响[J]. 水生生物学报, 2007, 31(3): 325-330. [18] Wang S H, Chen J C. The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei[J]. Fish and Shellfish Immunology, 2005, 19:191-204. [19] 陈萍, 王清印, 李健, 等. 溶藻弧菌对三疣梭子蟹溶菌酶和磷酸酶活性的影响[J]. 渔业科学进展, 2009, 30(2): 78-82. [20] 刘文珍, 邱德全. 溶藻弧菌对凡纳滨对虾血清中一氧化氮及氧自由基的影响[J]. 广东海洋大学学报, 2007, 27(3):60-63. [21] 王洪斌, 李士虎, 许冰, 等. 副溶血弧菌感染日本蟳后对几种免疫活性酶的影响响[J]. 水产科学, 2010, 29(11):653-656. [22] 王洪斌, 成明, 吴琴, 等. 鳗弧菌感染对日本蟳部分免疫活性因子的影响[J]. 水生生态杂志, 2012, 33(3):113-115. |
| 备注/Memo: | 收稿日期: 2013?02?07. 基金项目: 浙江省科技厅创新团队项目（2010R50029）; 宁波市创新团队项目（2011B81003）.第一作者: 周 伟（1987－）, 男, 河南信阳人, 在读硕士研究生, 主要研究方向: 海洋生物学. E-mail: holmeszhou@foxmail.com *通信作者: 徐善良（1962－）, 男, 浙江舟山人, 副研究员, 主要研究方向: 海洋生物学. E-mail: xushanliang@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
