卡罗藻有毒株与无毒株对菲律宾蛤仔的生长、存活及生理生化的影响
PDF下载 (330)吴延华,郭 贺,梅丽敏,潘渊博,徐建峰,周成旭.卡罗藻有毒株与无毒株对菲律宾蛤仔的生长、存活及生理生化的影响[J].宁波大学学报(理工版),2022,35(5):19-26.DOI:
WU Yanhua,GUO He,MEI Limin,PAN Yuanbo,XU Jianfeng,ZHOU Chengxu.Effects on the survival, growth and physio-biochemical activity of Ruditapes philippinarum by toxic and non-toxic Karlodinium species[J].Journal of Ningbo University(Natural Science & Engineering Edition),2022,35(5):19-26.DOI:
| Title: | Effects on the survival, growth and physio-biochemical activity of Ruditapes philippinarum by toxic and non-toxic Karlodinium species |
| 作者: | 吴延华, 郭 贺, 梅丽敏, 潘渊博, 徐建峰, 周成旭 |
| Author(s): | WU Yanhua, GUO He, MEI Limin, PAN Yuanbo, XU Jianfeng, ZHOU Chengxu |
| 关键词: | 剧毒卡罗藻; 周氏卡罗藻; 菲律宾蛤仔; 摄食; 糖原存储 |
| Keywords: | Karlodinium veneficum; Karlodinium zhouanum; Ruditapes philippinarum; grazing; glycogen content |
| 分类号: | Q89 |
| 文献标识码: | A |
| 摘要: | 剧毒卡罗藻对双壳类生理生化过程的影响尚待研究解明. 本文以菲律宾蛤仔为对象, 比较研究了细胞大小形态一致的剧毒卡罗藻和无毒周氏卡罗藻对菲律宾蛤仔的生长、存活、摄食生理以及糖原含量的影响. 结果显示, 2种卡罗藻喂养下, 菲律宾蛤仔稚贝均有生长, 但有毒组的生长指标和存活率均显著低于无毒组. 其滤水率和摄食率与藻密度呈近似幂函数相关, 15×105 cells·L-1为拐点藻密度; 小于或等于此藻密度时, 2组的摄食率和滤水率随密度增加而增加, 且无显著差异; 大于此密度时, 有毒藻组滤水率和摄食率均显著低于无毒藻组. 成贝较稚贝能耐受更高有毒藻密度. 摄食2种卡罗藻后, 菲律宾蛤仔各组织均快速累积糖原, 外套膜糖原含量最高, 变化最为显著. 但糖原累积量与藻密度有关, 低藻密度组中, 有毒藻组的糖原含量显著低于无毒藻组. 研究结果表明, 剧毒卡罗藻可以通过降低贝类摄食能力、干扰生化代谢等非急性致死过程来影响贝类的生长及存活. |
| Abstract: | How the dinoflagellate Karlodinum veneficum affect the growth and physio-biochemical metabolism of bivalves requires further research. In this study, two morphologically identical but of different toxicity species, namely the toxic K. veneficum, and the non-toxic K. zhouanum, were applied in the detection of their effects on the survival, growth, grazing activity and glycogen content of manila clam (Ruditapes philippinarum) juveniles or adults. The results show that both species support the clam for growth. But the growth and survival rates were significantly lower in the toxic algal group than those in the nontoxic one. Both filtration and ingestion rates changed along with the algal cell densities, resembling power function relationship in both algal groups. The algal density at the inflection point was 15×105 cells·L-1. In algal groups of lower or equal to this density, the filtration or ingestion rates increased along with the increased algal density. There was no significant difference in two algal groups. When algal cell densities were equal to (or higher than) 15×105 cells·L-1, the filtration and ingestion rates were significantly lower in toxic algal groups than those in non-toxic ones. The adult clam showed higher algal density tolerance than the juveniles. The glycogen contents in the starving adult clam increased rapidly after feeding both Karlodinium species of different densities. The changes were the most significant in mantle where the glycogen contents were the highest. The increase of glycogen content was related to the algal species and density. In low algal density group, the glycogen content in toxic group was significantly lower than that in non-toxic algal groups. This study showed that, toxic Karlodinium species may affect the growth and survival of the clam through certain non-acute processeses, such as by impacting the normal grazing process and interfering the biochemical metabolisms. |
| 参考文献 /References: | [1] Dame R F. Bivalve Filter Feeders in Estuarine and Coastal Ecosystem Processes[M]. Berlin, Heidelberg: Springer, 1993. [2] Rosa M, Ward J E, Holohan B A, et al. Physicochemical surface properties of microalgae and their combined effects on particle selection by suspension-feeding bivalve molluscs[J]. Journal of Experimental Marine Biology and Ecology, 2017, 486:59-68. [3] Hernroth B, Larsson A, Edebo L. Influence on uptake, distribution and elimination of Salmonella typhimurium in the blue mussel, Mytilus edulis, by the cell surface properties of the bacteria[J]. Journal of Shellfish Research, 2000, 19(1):167-174. [4] Ward J E, Targett N M. Influence of marine microalgal metabolites on the feeding behavior of the blue mussel Mytilus edulis[J]. Marine Biology, 1989, 101(3):313-321. [5] Newell R I E, Jordan S J. Preferential ingestion of organic material by the American oyster Crassostrea virginica[J]. Marine Ecology Progress Series, 1983, 13(1):47-53. [6] Riisg?rd H U, Kittner C, Seerup D F. Regulation of opening state and filtration rate in filter-feeding bivalves (Cardium edule, Mytilus edulis, Mya arenaria) in response to low algal concentration[J]. Journal of Experimental Marine Biology and Ecology, 2003, 284(1/2):105-127. [7] Rist S E, Assidqi K, Zamani N P, et al. Suspended micro-sized PVC particles impair the performance and decrease survival in the Asian green mussel Perna viridis [J]. Marine Pollution Bulletin, 2016, 111(1/2):213-220. [8] Lassudrie M, Hégaret H, Wikfors G H, et al. Effects of marine harmful algal blooms on bivalve cellular immunity and infectious diseases: A review[J]. Develop- mental & Comparative Immunology, 2020, 108:103660. [9] Binzer S B, Lundgreen R B C, Berge T, et al. The blue mussel Mytilus edulis is vulnerable to the toxic dinoflagellate Karlodinium armiger — Adult filtration is inhibited and several life stages killed[J]. PLoS One, 2018, 13(6):e0199306. [10] Deeds J R, Terlizzi D E, Adolf J E, et al. Toxic activity from cultures of Karlodinium micrum (=Gyrodinium galatheanum) (Dinophyceae) — A dinoflagellate associated with fish mortalities in an estuarine aquaculture facility[J]. Harmful Algae, 2002, 1(2):169- 189. [11] Garcés E, Fernandez M, Penna A, et al. Characterization of NW mediterranean Karlodinium spp. (Dinophyceae) strains using morphological, molecular, chemical, and physiological methodologies1[J]. Journal of Phycology, 2006, 42(5):1096-1112. [12] Lim H C, Leaw C P, Tan T H, et al. A bloom of Karlodinium australe (Gymnodiniales, Dinophyceae) associated with mass mortality of cage-cultured fishes in West Johor Strait, Malaysia[J]. Harmful Algae, 2014, 40:51-62. [13] Place A R, Bowers H A, Bachvaroff T R, et al. Karlodinium veneficum — The little dinoflagellate with a big bite[J]. Harmful Algae, 2012, 14:179-195. [14] Bachvaroff T R, Adolf J E, Squier A H, et al. Characterization and quantification of karlotoxins by liquid chromatography-mass spectrometry[J]. Harmful Algae, 2008, 7(4):473-484. [15] Cai P J, He S, Zhou C X, et al. Two new karlotoxins found in Karlodinium veneficum (strain GM2) from the East China Sea[J]. Harmful Algae, 2016, 58:66-73. [16] Kempton J W, Lewitus A J, Deeds J R, et al. Toxicity of Karlodinium micrum (Dinophyceae) associated with a fish kill in a South Carolina brackish retention pond[J]. Harmful Algae, 2002, 1(2):233-241. [17] Bj?rnland T, Tangen K. Pigmentation and morphology of a marine Gyrodinium (Dinophyceae) with a major carotenoid different from peridinin and fucoxanthin[J]. Journal of Phycology, 1979, 15(3):457-463. [18] Benico G, Takahashi K, Lum W M, et al. The harmful unarmored dinoflagellate Karlodinium in Japan and Philippines, with reference to ultrastructure and micropredation of Karlodinium azanzae sp. nov. (Kareniaceae, Dinophyceae)[J]. Journal of Phycology, 2020, 56(5):1264-1282. [19] Cen J Y, Wang J Y, Huang L F, et al. Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom[J]. Journal of Oceanology and Limnology, 2021, 39(1):242-258. [20] Dai X F, Lu D D, Guan W B, et al. Newly recorded Karlodinium veneficum dinoflagellate blooms in stratified water of the East China Sea[J]. Deep Sea Research Part II: Topical Studies in Oceanography, 2014, 101:237-243. [21] Hallegraeff G, Dorantes-Aranda J J, Mardones J, et al. Review of progress in our understanding of fish-killing microalgae: Implications for management and mitigation [C]//International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO 2017. [22] Zhu X J, Zhou C X, Meng R, et al. Biochemical characteristics support the recently described species Karlodinium zhouanum (Gymnodiniales, Dinophyceae) [J]. Phycological Research, 2020, 68(1):14-22. [23] Huang H L, Shao Q W, Zhu X J, et al. Distribution of Karlodinium veneficum in the coastal region of Xiangshan Bay in the East China Sea, as detected by a real-time quantitative PCR assay of ribosomal ITS sequence[J]. Harmful Algae, 2019, 81:65-76. [24] Park T G, Kang Y S, Park Y T, et al. Detection of fish killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum (Dinophyceae) in the East China Sea by real-time PCR[J]. Harmful Algae, 2009, 24(2):105-110. [25] Xu X, Yu Z, Cheng F, et al. Molecular diversity and ecological characteristics of the eukaryotic phytoplankton community in the coastal waters of the Bohai Sea, China[J]. Harmful Algae, 2017, 61:13-22. [26] 国家统计局. 中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020. [27] Coughlan J. The estimation of filtering rate from the clearance of suspensions[J]. Marine Biology, 1969, 2(4):356-358. [28] 范嗣刚, 姜松, 黄桂菊, 等. 合浦珠母贝和大珠母贝稚贝摄食率及摄食节律的比较研究[J]. 水产科技情报, 2015, 42(1):6-10. [29] Carroll N V, Longley R W, Roe J H. The determination of glycogen in liver and muscle by use of anthrone reagent[J]. Journal of Biological Chemistry, 1956, 220(2):583-593. [30] 付家想, 蓝文陆, 李天深, 等. 香港巨牡蛎对3种浮游植物摄食率和滤清率的研究[J]. 海洋学报, 2017, 39(8):62-69. [31] Shumway S E, Cucci T L. The effects of the toxic dinoflagellate Protogonyaulax tamarensis on the feeding and behaviour of bivalve molluscs[J]. Aquatic Toxicology, 1987, 10(1):9-27. [32] Shumway S E, Cembella A D. The impact of toxic algae on scallop culture and fisheries[J]. Reviews in Fisheries Science, 1993, 1(2):121-150. [33] Samain J F. Review and perspectives of physiological mechanisms underlying genetically-based resistance of the Pacific oyster Crassostrea gigas to summer mortality [J]. Aquatic Living Resources, 2011, 24(3):227-236. [34] Hardie D G, Sakamoto K. AMPK: A key sensor of fuel and energy status in skeletal muscle[J]. Physiology, 2006, 21(1):48-60. [35] Uzaki N, Kai M, Aoyama H, et al. Changes in mortality rate and glycogen content of the Manila clam Ruditapes philippinarum during the development of oxygen- deficient waters[J]. Fisheries Science, 2003, 69(5):936-943. [36] Carefoot T H, Qian P Y, Taylor B E, et al. Effect of starvation on energy reserves and metabolism in the Northern abalone, Haliotis kamtschatkana[J]. Aquaculture, 1993, 118(3/4):315-325. [37] Galimany E, Place A R, Jutson M, et al. The effects of feeding Karlodinium veneficum (PLY # 103; Gymnodinium veneficum Ballantine) to the blue mussel Mytilus edulis[J]. Harmful Algae, 2008, 7(1):91-98. [38] Tatsuya Y. Seasonal changes in glycogen contents in various tissues of the edible bivalves, pen shell Atrina lischkeana, ark shell Scapharca kagoshimensis, and manila clam Ruditapes philippinarum in west Japan[J]. Journal of Marine Biology, 2015, 2015:1-5. |
| 备注/Memo: | 收稿日期: 2021－11－10. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家重点研发计划（2018YFD0900702）; 科技基础资源调查专项（2018FY100206）. 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/第一作者: 吴延华（1997－）, 男, 安徽怀宁人, 在读硕士研究生, 主要研究方向: 双壳贝类的代谢. E-mail: fireworks555@163.com *通信作者: 周成旭（1968－）, 女, 四川什邡人, 研究员, 主要研究方向: 微藻生物技术. E-mail: zhouchengxu@nbu.edu.c |
