鲤春病毒血症的检测技术与防控研究进展
PDF下载 (87)葛明峰,徐胜威,景宏丽,王建平,高隆英,付志明,王雯琼.鲤春病毒血症的检测技术与防控研究进展[J].宁波大学学报(理工版),2025,38(1):100-106.DOI:10.20098/j.cnki.1001-5132.2024.0728
GE Mingfeng,XU Shengwei,JING Hongli,WANG Jianping,GAO Longying,FU Zhiming,WANG Wenqiong.Research progress on detection technology and prevention of spring viremia of carp[J].Journal of Ningbo University(Natural Science & Engineering Edition),2025,38(1):100-106.DOI:10.20098/j.cnki.1001-5132.2024.0728
| Title: | Research progress on detection technology and prevention of spring viremia of carp |
| 作者: | 葛明峰, 徐胜威, 景宏丽, 王建平, 高隆英, 付志明, 王雯琼 |
| Author(s): | GE Mingfeng, XU Shengwei, JING Hongli, WANG Jianping, GAO Longying, FU Zhiming, WANG Wenqiong |
| 关键词: | 鲤春病毒血症; 鲤春病毒血症病毒; 检测技术; 防治方法 |
| Keywords: | spring viremia of carp; spring viremia of carp virus; detection technology; prevention and control methods |
| 分类号: | S941.41 |
| DOI: | 10.20098/j.cnki.1001-5132.2024.0728 |
| 文献标识码: | A |
| 摘要: | 鲤春病毒血症(Spring Viremia of Carp, SVC)是由鲤春病毒血症病毒(Spring Viraemia of Carp Virus, SVCV)引起的一种急性、出血性的病毒性疾病. SVC是影响鲤科鱼类养殖产业及观赏鱼类贸易较为严重的疾病, 目前尚无有效的治疗药物, 只能通过消毒、检验检疫、日常管理等方式进行预防. 对该病的诊断主要以《鲤春病毒血症诊断规程》(GB/T 15805.5—2018)为依据, 同时基于重组酶聚合酶扩增(Recombinase Polymerase Amplification, RPA)等快速检测技术和试剂盒的开发与应用为SVC防治提供新方法. 为此, 针对SVC的流行特点、临床症状、近几年监测情况、病原检测技术、致病机理、疫苗和药物防控等方面进行了梳理和总结, 并展望了SVC的防治策略和方向, 为今后更深入研究该疫病提供参考. |
| Abstract: | The spring viremia of carp (SVC) is an acute and hemorrhagic viral disease caused by spring viremia of carp virus (SVCV). SVC is a serious disease that affects the carp fish farming industry and the trade of ornamental fish. Currently, there are no effective therapeutic drugs. Prevention can only be done through measures such as disinfection, inspection and quarantine, daily management, as well as others. The diagnosis of SVC is mainly based on Protocol of diagnosis for spring viremia of carp (GB/T 15805.5-2018). The development and application of rapid detection technologies and kits based on Recombinant Polymerase Amplification (RPA) provide new means for the prevention and treatment of SVC. This article summarizes the epidemic characteristics, clinical symptoms, monitoring in recent years, pathogen detection technology, pathogenic mechanisms, vaccines, and drug prevention and control of SVC. Meanwhile, the preventive treatment strategies and directions of SVC are also discussed, providing reference for the more in-depth research on this disease in the future. |
| 参考文献 /References: | [1].AHNE W, BJORKLUND H V, ESSBAUER S, et al. Spring viremia of carp (SVC)[J]. Diseases of Aquatic Organisms, 2002, 52(3):261-272. [2].PADHI A, VERGHESE B. Detecting positively selected codons in the glycoprotein of spring viraemia of carp virus (SVCV) isolates from the USA and China[J]. Journal of Fish Diseases, 2008, 31(10):785-791. [3].LIU H, GAO L, SHI X, et al. Isolation of spring viraemia of carp virus (SVCV) from cultured koi (Cyprinus carpio koi) and common carp (C. carpio carpio) in P. R. China[J]. Bulletin of the European Association of Fish Pathologists, 2004, 24(4):194-202. [4].WARG J V, DIKKEBOOM A L, GOODWIN A E, et al. Comparison of multiple genes of spring viremia of carp viruses isolated in the United States[J]. Virus Genes, 2007, 35(1):87-95. [5].PADHI A, VERGHESE B. Molecular evolutionary and epidemiological dynamics of a highly pathogenic fish rhabdovirus, the spring viremia of carp virus (SVCV)[J]. Veterinary Microbiology, 2012, 156(1/2):54-63. [6].BJÖRKLUND H V, HIGMAN K H, KURATH G. The glycoprotein genes and gene junctions of the fish rhabdoviruses spring viremia of carp virus and hirame rhabdovirus: analysis of relationships with other rhabdoviruses[J]. Virus Research, 1996, 42(1/2):65-80. [7].AHNE W. Argulus foliaceus L. and Piscicola geometra L. as mechanical vectors of spring viraemia of carp virus (SVCV)[J]. Journal of Fish Diseases, 1985, 8(2):241-242. [8].王姝, 徐立蒲, 王静波, 等. 鲤春病毒血症风险分析[J]. 北京农业, 2012(18):107-110. [9].蒋昕彧, 王啸宇, 裴超, 等. 鲤病毒性疾病发生与防控的研究进展[J]. 南方农业学报, 2021, 52(2):509-517. [10].FIJAN N. Spring viraemia of carp and other viral diseases and agents of warm-water fish[J]. Fish Diseases and Disorders, 1999, 3:177-244. [11].GHASEMI M, ZAMANI H, HOSSEINI S M, et al. Caspian white fish (Rutilus frisii kutum) as a host for spring viraemia of carp virus[J]. Veterinary Micro- biology, 2014, 170(3/4):408-413. [12].MISK E, GARVER K, NAGY E, et al. Pathogenesis of spring viremia of carp virus in emerald shiner Notropis atherinoides Rafinesque, fathead minnow Pimephales promelas Rafinesque and white sucker Catostomus commersonii (Lacepede)[J]. Journal of Fish Diseases, 2016, 39(6):729-739. [13].农业农村部渔业渔政管理局, 全国水产技术推广总站. 2022我国水生动物重要疫病状况分析[M]. 北京: 中国农业出版社, 2022. [14].农业农村部渔业渔政管理局, 全国水产技术推广总站. 2022中国水生动物卫生状况报告[M]. 北京: 中国农业出版社, 2022. [15].王姝, 徐立蒲, 王小亮, 等. 北京地区2007—2015年鲤春病毒血症病毒感染流行病学研究[J]. 病毒学报, 2017, 33(4):593-597. [16].田飞焱, 欧阳敏, 谢世红, 等. 江西省2005—2017年鲤春病毒血症病毒感染流行病学研究[J]. 水产学杂志, 2019, 32(2):32-36. [17].邵玲, 肖雨, 汤茜, 等. 上海地区2013—2018年鲤春病毒血症病毒的分离和进化特征分析[J]. 水产科技情报, 2019, 46(5):253-258. [18].付峰, 刘荭, 黄健, 等. 鲤春病毒血症病毒(SVCV)的研究进展[J]. 中国水产科学, 2006, 13(2):328-334. [19].GB/T 15805.5—2018. 鲤春病毒血症诊断规程[S]. [20].BUSTIN S A. Developments in real-time PCR research and molecular diagnostics[J]. Expert Review of Molecular Diagnostics, 2010, 10(6):713-715. [21].安伟, 肖雨, 张明辉, 等. 鲤春病毒血症病毒SYBR GreenⅠ荧光定量RT-PCR方法的建立[J]. 中国预防兽医学报, 2015, 37(9):699-702. [22].SHAO L, XIAO Y, HE Z K, et al. An N-targeting real- time PCR strategy for the accurate detection of spring viremia of carp virus[J]. Journal of Virological Methods, 2016, 229:27-34. [23].JAMES A, MACDONALD J. Recombinase polymerase amplification: emergence as a critical molecular technology for rapid, low-resource diagnostics[J]. Expert Review of Molecular Diagnostics, 2015, 15(11):1475- 1489. [24].梁君妮, 尹伟力, 谢爽, 等. 重组酶聚合酶扩增技术快速检测鲤春病毒血症病毒(SVCV)方法的建立[J]. 中国预防兽医学报, 2019, 41(10):1037-1040. [25].田城城, 兰文升, 叶仕根. 基于RPA的鲤春病毒血症病毒核酸检测技术的建立与评价[J]. 中国动物检疫, 2020, 37(2):99-106. [26].CONG F, ZENG F W, WU M L, et al. Development of a real-time reverse transcription recombinase polymerase amplification assay for rapid detection of spring viremia of carp virus[J]. Molecular and Cellular Probes, 2020, 50:101494. [27].刘瑶, 尹伟力, 张四化, 等. 鲤春病毒血症病毒焦磷酸测序检测技术的建立[J]. 水产科学, 2017, 36(4):449- 455. [28].SALEH M, SOLIMAN H, SCHACHNER O, et al. Direct detection of unamplified spring viraemia of carp virus RNA using unmodified gold nanoparticles[J]. Diseases of Aquatic Organisms, 2012, 100(1):3-10. [29].TONG G X, WEI X X, YIN W L, et al. Development of a liquid chip technique to simultaneously detect spring viremia of carp virus, infectious hematopoietic necrosis virus, and viral hemorrhagic septicemia of salmonids[J]. Journal of AOAC International, 2017, 100(1):159-164. [30].朱裕敏, 吴江, 廖立珊, 等. 鲤春病毒血症病毒RT- RAA-LFD检测方法的建立[J]. 中国预防兽医学报, 2023, 45(9):916-921. [31].LI C, LI L J, JIN L, et al. Heme Oxygenase-1 inhibits spring viremia of carp virus replication through carbon monoxide mediated cyclic GMP/Protein kinase G signaling pathway[J]. Fish & Shellfish Immunology, 2018, 79:65-72. [32].LIU L, TU X, SHEN Y F, et al. The replication of spring viraemia of carp virus can be regulated by reactive oxygen species and NF-κB pathway[J]. Fish & Shellfish Immunology, 2017, 67:211-217. [33].李聪. 血红素加氧酶1抑制鲤春病毒血症病毒的机制研究[D]. 武汉: 华中农业大学, 2018. [34].SHAO J H, HUANG J, GUO Y N, et al. Up-regulation of nuclear factor E2-related factor 2 (Nrf2) represses the replication of SVCV[J]. Fish & Shellfish Immunology, 2016, 58:474-482. [35].YANG Y, HUANG J, LI L J, et al. Up-regulation of nuclear factor E2-related factor 2 upon SVCV infection[J]. Fish & Shellfish Immunology, 2014, 40(1):245-252. [36].YUAN J F, SU N, WANG M, et al. Down-regulation of heme oxygenase-1 by SVCV infection[J]. Fish & Shellfish Immunology, 2012, 32(2):301-306. [37].王雪丽. 鲤春病毒血症病毒N蛋白通过自噬-溶酶体途径降解STING抑制IFN产生的分子机制[D]. 大连: 大连海洋大学, 2023. [38].张蕾. 宿主蛋白HSP47对鲤春病毒血症病毒侵染的影响[D]. 长春: 吉林农业大学, 2020. [39].王楠. 热休克蛋白HSP90对鲤春病毒血症病毒复制影响的初步研究[D]. 长春: 吉林农业大学, 2021. [40].李陈. 宿主蛋白HSC70和PHB与鲤春病毒血症病毒G蛋白相互作用及其在病毒感染中的作用研究[D]. 武汉: 华中农业大学, 2023. [41].香凤. 鲤春病毒血症病毒的受体捕获及初步筛选[D]. 深圳: 深圳大学, 2020. [42].林婧楠, 赵景壮, 卢彤岩, 等. 鲤春病毒血症病毒的研究进展[J]. 水产学杂志, 2018, 31(5):44-49, 57. [43].林婧楠, 赵景壮, 刘淼, 等. 鲤春病毒血症病毒糖蛋白酵母表面展示系统的建立[J]. 水生生物学报, 2019, 43(5):977-982. [44].郭晟. 鲤春病毒血症仿生疫苗构建及其免疫效果评价[D]. 咸阳: 西北农林科技大学, 2022. [45].EMBREGTS C W E, RIGAUDEAU D, TACCHI L, et al. Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine[J]. Fish & Shellfish Immunology, 2019, 85:66-77. [46].刘镭. 香豆素类化合物抗鲤春病毒血症病毒活性及作用机制研究[D]. 咸阳: 西北农林科技大学, 2018. [47].宋大伟. 含氮杂环类香豆素衍生物抗鲤春病毒血症病毒活性与机制初探[D]. 宁波: 宁波大学, 2021. [48].沈毓峰. 牛蒡子苷元衍生物抗鲤春病毒血症病毒活性及机制研究[D]. 咸阳: 西北农林科技大学, 2021. [49].曾荣博. 抗鲤春病毒血症病毒和大口黑鲈弹状病毒发酵中草药的筛选及其效果评价[D]. 武汉: 华中农业大学, 2023. [50].李钰. 利用斑马鱼模型研究3种天然多糖抗鲤春病毒血症病毒效应机制[D]. 武汉: 华中农业大学, 2021. [51].刘佳, 卢玉婷, 刘芸娜, 等. 维生素C体外抑制SVCV入侵鲤鱼上皮瘤细胞的初步研究[J]. 中国兽医杂志, 2021, 57(10):10-14. [52].李泽明. 鲤春病毒血症病毒M单克隆抗体的制备及鲤天然免疫蛋白TRIM32特性初步研究[D]. 武汉: 华中农业大学, 2015. [53].王姝, 徐立蒲, 王小亮, 等. 鲤浮肿病毒混合感染的监测与分析[J]. 检验检疫学刊, 2018, 28(4):6-9. [54].张晨. 靶向性碳纳米管载疫苗系统防治鲤春病毒血症研究[D]. 咸阳: 西北农林科技大学, 2019. |
| 备注/Memo: | 收稿日期: 2024−07−30. 宁波大学学报(理工版)网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市公益类科技计划项目(2022S169). 第一作者: 葛明峰, 工程师, 主要研究方向: 水生生物病害防治. E-mail: 910342950@qq.com *通信作者: 徐胜威, 工程师, 主要研究方向: 水生生物病害防治. E-mail: 546207546@qq.com 宁波大学学报(理工版)网址:http://journallg.nbu.edu.cn/ |