琼胶寡糖对坛紫菜抗烂性的诱导效应
PDF下载 (334)刘方方,骆其君,陈海敏 *,严小军.琼胶寡糖对坛紫菜抗烂性的诱导效应[J].宁波大学学报(理工版),2014,27(04):12-15.DOI:
LIU Fang-fang,LUO Qi-jun,CHEN Hai-min *,YAN Xiao-jun.Rot-resistance Effect of Oligoagar on Disease of Pyropia haitanensis[J].Journal of Ningbo University(Natural Science & Engineering Edition),2014,27(04):12-15.DOI:
| Title: | Rot-resistance Effect of Oligoagar on Disease of Pyropia haitanensis |
| 作者: | 刘方方, 骆其君, 陈海敏 *, 严小军 |
| Author(s): | LIU Fang-fang, LUO Qi-jun, CHEN Hai-min *, YAN Xiao-jun |
| 关键词: | 琼胶寡糖; 激发子; 坛紫菜; 腐烂率; 养殖 |
| Keywords: | oligoagar; elicitor; Pyropia haitanensis; rot; aquaculture |
| 分类号: | S946.2 |
| 文献标识码: | A |
| 摘要: | 为研究琼胶寡糖对室内和海区养殖坛紫菜的抗烂效应, 以不同质量浓度琼胶寡糖处理坛紫菜叶片, 通过观察叶片的腐烂情况, 筛选坛紫菜最佳处理方法, 并将其应用于海区养殖坛紫菜. 研究结果表明: 100μg.mL-1琼胶寡糖处理2次, 2d一次, 每次2h, 能诱导坛紫菜最佳抗烂作用, 使腐烂率下降37.31%(P<0.01), 且只有少量细胞萎缩坏死. 琼胶寡糖处理坛紫菜叶片培养至40d时完全腐烂, 较空白组延后13d. 将该条件应用于海区坛紫菜养殖, 能够提高坛紫菜的叶片宽度、鲜重, 并使尖端腐烂率下降19.36%. |
| Abstract: | The anti-rot effect of oligoagar on Pyropia haitanensis in both the laboratory and aquaculture is investigated. The concentration of oligoagar and times for treatment are optimized by examining the percentage of rot of thalli. The optimized conditions are then applied for aquaculture of P. haitanensis. Results indicate that, processed twice with 2 hours each and once in 2 days, the elicitation of P. haitanensis with 100 μg.mL-1 oligoagars can produce the optimal rot-resistance effect. The rot percentage of algal thalli decrease by 37.31% (P<0.01) with only a insignificant number of dead cells. After 40-day culturing, it is found that the thalli of two groups are rotten severely, but the rot in the oligoagar group with anti-rot treatment is delayed for more than 13 days. The aquaculture results reveal that the width and fresh weight of treated group are increased, and the rot percentage of algal thallus tip decrease by 19.36%. |
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| 备注/Memo: | 收稿日期: 2014-03-08. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 国家星火重大项目（2013GA701001）; 宁波市科技攻关项目（201201C1011016）.第一作者: 刘方方（1979－）, 女, 江苏徐州人, 在读硕士研究生, 主要研究方向: 海洋生物学. E-mail: lff8@163.com*通信作者: 陈海敏（1975－）, 女, 浙江瑞安人, 博士/研究员, 主要研究方向: 海洋药物等. E-mail: chenhaimin@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
