喉癌患者血浆及组织中长链非编码RNA的检测及临床意义
PDF下载 (421)王 剑1,崔 翔1,李 群2,陈声灿1,沈志森1,2*,郭俊明1*.喉癌患者血浆及组织中长链非编码RNA的检测及临床意义[J].宁波大学学报(理工版),2016,29(3):123-127.DOI:
WANG Jian 1,CUI Xiang 1,LI Qun 1,CHEN Sheng-can 1,SHEN Zhi-sen 1,2*,GUO Jun-ming 1*. Detection of Long Non-Coding RNAs in Plasma and Tissues with Larynx Squamous Cell Carcinoma and its Clinical Significance
| Title: | Detection of Long Non-Coding RNAs in Plasma and Tissues with Larynx Squamous Cell Carcinoma and its Clinical Significance |
| 作者: | 王 剑1, 崔 翔1, 李 群2, 陈声灿1, 沈志森1, 2*, 郭俊明1* |
| Author(s): | WANG Jian 1, CUI Xiang 1, LI Qun 1, CHEN Sheng-can 1, SHEN Zhi-sen 1, 2*, GUO Jun-ming 1* |
| 关键词: | 长链非编码RNA; 喉鳞状细胞癌; 血浆; 组织 |
| Keywords: | RP11-552E20.1-001; LncRNA; larynx squamous cell carcinoma; plasma; tissues |
| 分类号: | Q279 |
| 文献标识码: | A |
| 摘要: | 为研究长链非编码RNA(Long non-coding RNA, LncRNA)RP11-552E20.1-001在喉鳞状细胞癌患者血浆和组织中的表达水平及临床价值, 采用实时定量聚合酶链反应(Real-time PCR)方法, 检测67例喉癌患者手术前后一周的血浆, 以声带息肉患者血浆作对照, 观察喉癌组织、转移的淋巴结和癌旁正常黏膜组织的表达情况, 分析其与喉癌临床病理学特征的联系, 构建受试者工作特征曲线(Receiver Operating Characteristic Curve, ROC), 探讨其诊断价值. 研究发现, 喉癌患者血浆LncRNA表达水平高于声带息肉患者, 且术后较术前显著降低; 喉癌组织中该链表达较癌旁组织上调, 转移的淋巴结比原发病灶表达水平更高; 术前血浆及喉癌组织中该链表达与肿瘤分化程度、淋巴结转移和TNM分期显著相关. ROC曲线下面积为0.734, 其敏感性为80.6%, 特异性为67.2%. 提示该LncRNA可能是喉癌诊断的潜在标记物, 并在喉癌的侵袭、转移过程中起重要作用. |
| Abstract: | This paper contributes to investigating LncRNA (RP11-552E20.1-001) expression level in patients’ plasma and tissues with larynx squamous cell carcinoma (LSCC), and to determining its clinical significance. Using real-time quantitative PCR, 67 cases of preoperative and postoperative one-week of LSCC plasma samples, the vocal cord polyp patients’ plasma, LSCC tissues, metastatic lymph nodes and adjacent normal laryngeal mucosa are tested for quantitative analysis of RP11-552E20.1-001. The relationship between the expression of LncRNA (RP11-552E20.1-001) and clinicopathological features is analyzed. A receiver constructing the characteristic (ROC) curve is rendered to check the diagnostic value. It is found that the levels of RP11-552E20.1-001 expression are significantly higher in LSCC patients’ plasma than the control, and they decrease notably after the operation compared with the preoperative levels. The relative expression of LncRNA (RP11-552E20.1-001) in LSCC tissues is significantly higher than that in adjacent tissues, and metastatic lymph nodes has the higher expression of LncRNA relative to primary lesion. The expression of LncRNA also shows the significant correlation with tumor differentiation, lymph node metastasis and TNM staging in preoperative plasma and LSCC tissues. The area under the ROC curve accounts up to 0.734. Its sensitivity and specificity are found to be 80.6% and 67.2%, respectively. LncRNA (RP11-552E20.1-001) may be a potential marker for the diagnosis of laryngeal cancer and play an important role in the invasion and metastasis of LSCC. |
| 参考文献 /References: |
[1]
DU L, LI H, ZHU C, et al. Incidence and mortality of laryngeal cancer in China, 2011[J]. Chin J Cancer Res, 2015, 27(1):52-58.
[2]
YANG Q Q, DENG Y F. Long non-coding RNAs as novel biomarkers and therapeutic targets in head and neck cancers[J]. Int J Clin Exp Pathol, 2014, 7(4):1286-1292.
[3]
QI P, DU X. The long non-coding RNAs, a new cancer diagnostic and therapeutic gold mine[J]. Mod Pathol, 2013, 26(2):155-165.
[4]
TANG H, WU Z, ZHANG J, et al. Salivary LncRNA as a potential marker for oral squamous cell carcinoma diagnosis[J]. Mol Med Rep, 2013, 7(3):761-766.
[5]
TONG Y S, WANG X W, ZHOU X L, et al. Identification of the long non-coding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma[J]. Mol Cancer, 2015, 14(1):3-10.
[6]
RIVAS A, BURZIO V, LANDERER E, et al. Determination of the differential expression of mitochondrial long non-coding RNAs as a noninvasive diagnosis of bladder cancer[J]. BMC Urology, 2012, 12(1):1-8.
[7]
SHAO Y, YE M, JIANG X, et al. Gastric juice long noncoding RNA used as a tumor marker for screening gastric cancer[J]. Cancer, 2014, 120(21):3320-3328.
[8]
EDGE S B, COMPTON C C. The american joint committee on cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM[J]. Annals of Surgical Oncology, 2010, 17(6):1471-1474.
[9]
SHEN Z, LI Q, DENG H, et al. Long non-coding RNA profiling in laryngeal squamous cell carcinoma and its clinical significance: Potential biomarkers for LSCC[J]. PLoS One, 2014, 9(9):108-113.
[10]
SPRINGER N L, SMITH E, BROOKS M B, et al. Flow cytometric detection of circulating platelet-derived microparticles in healthy adult horses[J]. Am J Vet Res, 2014, 75(10):879-885.
[11]
HUANG X, YUAN T, TSCHANNEN M, et al. Characterization of human plasma-derived exosomal RNAs by deep sequencing[J]. BMC Genomics, 2013, 14(1):1-14.
[12] WANG J, ZHOU Y, LU J, et al. Combined detection of serum exosomal miR-21 and HOTAIR as diagnostic and prognostic biomarkers for laryngeal squamous cell carcinoma[J]. Med Oncol, 2014, 31(9):513-518. |
| 备注/Memo: | 收稿日期: 2016?03?25. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省自然科学基金（ LY14H160003 ） ; 宁波市科技创新团队（ 2012B82019 ） ; 宁波市重大择优委托项目（ 2012C5015 ） ; 宁波市自然科学基金（ 2012A610217 ） ; 浙江省医药卫生科技计划项目（ 2012ZDA042 ） ; 浙江省医药卫生省部培育计划（ 2014PYA017 ） . 第一作者: 王剑（1989－）, 男, 浙江台州人, 在读硕士研究生, 主要研究方向: 头颈肿瘤疾病. E-mail: wangjian_nbu@163.com *通信作者: 沈志森（1959－）, 男, 浙江宁波人, 主任医师, 主要研究方向: 头颈肿瘤疾病. E-mail: szs7216@163.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
