乌鳢伊丽莎白菌的分离鉴定及致病性研究
PDF下载 (845)刘晓琳,梅 寒,卜令飞,金 珊 *,赵青松,陈寅儿.乌鳢伊丽莎白菌的分离鉴定及致病性研究[J].宁波大学学报(理工版),2018,31(2):1-7.DOI:
LIU Xiao-lin,MEI Han,BU Ling-fei,JIN Shan *,ZHAO Qing-song,CHEN Yin-er.Isolation and pathogenicity study of Elizabethkingia sp. from Channa argus[J].Journal of Ningbo University(Natural Science & Engineering Edition),2018,31(2):1-7.DOI:
| Title: | Isolation and pathogenicity study of Elizabethkingia sp. from Channa argus |
| 作者: | 刘晓琳, 梅 寒, 卜令飞, 金 珊 *, 赵青松, 陈寅儿 |
| Author(s): | LIU Xiao-lin, MEI Han, BU Ling-fei, JIN Shan *, ZHAO Qing-song, CHEN Yin-er |
| 关键词: | 伊丽莎白菌; 乌鳢; 致病性; 药物敏感性 |
| Keywords: | 16S rDNA; Elizabethkingia sp.; Channa argus; pathogenicity; drug sensitivity test |
| 分类号: | S941.42 |
| 文献标识码: | A |
| 摘要: | 为解明宁波某养殖场乌鳢死亡的原因, 从患典型症状病鱼体内分离到1株细菌WLY01, 经形态学观察、生理生化表型鉴定和16S rDNA序列分析, 鉴定该菌为伊丽莎白菌(Elizabethkingia sp.). 该菌最适生长温度为37℃, 其对绵羊血、小鼠血、乌鳢血和鲫鱼血等都有较强溶血活性, 呈β溶血. 人工感染试验表明: 该菌主要通过鱼体表受伤部位感染, 发病症状和死亡速度与创伤程度呈明显相关性, 其8d的LD50为1.69×106cfu·g-1, 对乌鳢具有较强致病性. 药敏试验显示: 该菌对米诺环素、恩诺沙星、利福平、强力霉素、磺胺异 唑、依诺沙星等6种药物较敏感, 因此, 可把米诺环素、利福平、强力霉素、磺胺异 唑等作为治疗鱼类伊丽莎白菌病的候选药物. |
| Abstract: | In order to identify the pathogens of the Channa argus batch death from a farm in Ningbo, the dominant bacteria (WLY01) was isolated from the diseased fish. It was identified as Elizabethkingia sp., based on morphological, biochemical and physiological characteristics and sequencing of 16S rDNA. The strain has strong hemolytic activity with β hemolysis against sheep blood, mouse blood, snakehead blood and crucian carp blood. Artificial infection experiment demonstrated that the isolates were mainly infected by the wound surface of the fish. The symptoms and death rate were significantly correlated with the degree of trauma. The LD50 of the strain was 1.69×106cfu·g-1, which was highly pathogenic to the Channa argus. The result of drug sensitivity test showed that the isolates were sensitive to the Minocycline, Enrofloxacin, Rifampin, Doxycycline, Sulfafurazole, and Enoxacin. It is suggested that Minocycline, Rifampin, Doxycycline and Sulfafurazole can be used as candidate drugs for treatment of fish Elizabethkingia. |
| 参考文献 /References: | [1] 林永贺, 邹记兴, 林显道. 乌鳢营养研究进展[J]. 广东饲料, 2009, 18(3):42-44. [2] Lau S K, Chow W N, Foo C H, et al. Elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality[EB/OL]. [2016- 10-18]. http://www.nature.com/articles/srep26045. [3] 房海, 陈翠真, 张晓君. 水产养殖动物病原细菌学[M]. 北京: 中国农业出版社, 2010:499-517. [4] Kim K K, Kim M K, Lim J H, et al. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2005, 55(3):1287-1293. [5] Kampfer P, Matthews H, Glaeser S P, et al. Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae[J]. International Journal of Systematic and Evolutionary Microbiology, 2011, 61: 2670-2675. [6] 赵乃 , 苑广盈. 医学细菌名称及分类鉴定[M]. 3版. 济南: 山东大学出版社, 2013:115-120. [7] 王鑫毅, 韩艳楠, 金珊, 等. 乌鳢产吲哚金黄杆菌的鉴定及其胞外产物特性分析[J]. 水生生物学报, 2016, 40(3):641-646. [8] 韩艳楠, 余银春, 王鑫毅, 等. 牛蛙致病变形菌的鉴定及其敏感药物筛选[J]. 微生物学通报, 2016, 43(6): 1280-1287. [9] 余银春, 韩艳楠, 王鑫毅, 等. 湘云鲫体表出血溃烂病病原的鉴定及防治药物筛选[J]. 农业灾害研究, 2015, 5(3):28-30. [10] 熊浩明, 魏柏青, 魏荣杰, 等. 用SPSS软件计算鼠疫菌半数致死量(LD50)[J]. 中国人兽共患病学报, 2013, 29(11):1127-1130. [11] Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement[S]. CLSI M100-S23, 2013. [12] Bernardet J F, Vancanneyt M, Matte-Tailliez O, et al. Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals[J]. Systematic and Applied Microbiology, 2005, 28(7):640-660. [13] 陈爱平, 江育林, 钱冬, 等. 蛙脑膜炎败血金黄杆菌病[J]. 中国水产, 2012(5):51-52. [14] 陈耀明, 胡永强, 周以凤, 等. 牛蛙脑膜炎脓毒性黄杆菌病[J]. 水产科技情报, 1994, 21(1):11-12. [15] 叶雪平, 杨广智, 罗毅志. 牛蛙脑膜炎(歪脖子病)病原分离及防治技术研究[J]. 浙江水产学院学报, 1996, 15(4):301-304. [16] 张奇亚, 李正秋, 吴玉琛. 美国青蛙“旋游症”的病原菌分离鉴定及组织病理学观察[J]. 中国兽医学报, 1999, 19(2):152-155. [17] 周永灿, 朱传华, 陈国华, 等. 虎纹蛙白内障病病原的分离鉴定及其免疫防治[J]. 上海水产大学学报, 2001, 10(1):16-21. [18] 蔡完其, 孙佩芳, 朱泽闻, 等. 中华鳖脑膜炎败血性黄杆菌病的研究[J]. 水产科技情报, 1997, 24(4):156-161. [19] 周煜华, 何成伟. 黄鳝鱼脑膜炎脓毒性黄杆菌的分离与鉴定[J]. 广西畜牧兽医, 1998, 14(3):5-7. [20] 黄志坚, 何建国, 翁少萍, 等. 鳜鱼细菌性病原的分离鉴定及致病性初步研究[J]. 微生物学通报, 1999, 26(4):241-246. [21] Frank T, Gody J C, Nguyen L B L, et al. First case of Elizabethkingia anophelis meningitis in the Central African Republic[J]. The Lancet, 2013, 381(9880):1876. [22] Li Y, Kawamura Y N, Naka T, et al. Chryseobacterium miricola sp nov. a novel species isolated from condensation water of space station Mir[J]. Systematic & Applied Microbiology, 2003, 26(4):523-528. [23] Rossati A, Kroumova V, Bargiacchi O, et al. Elizabethkingia miricola bacteriemia in a young woman with acute alcoholic pancreatitis[J]. Presse Medicale, 2015, 44(10): 1071-1072. [24] Breurec S, Criscuolo A, Diancourt L, et al. Genomic epidemiology and global diversity of the emerging bacterial pathogen Elizabethkingia anophelis[EB/OL]. [2016-11-05]. http://www.nature.com/articles/srep30379. [25] Sáez-Llorens X, McCracken G H. Bacterial meningitis in children[J]. The Lancet, 2003, 361(9375):2139-2148. [26] Gonzalez L J, Vila A J. Carbapenem resistance in Elizabethkingia meningoseptica is mediated by metallo-β- lactamase BlaB[J]. Antimicrobial Agents and Chemotherapy, 2012, 56(4):1686-1692. |
| 备注/Memo: | 收稿日期: 2017－06－21. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 海洋公益性行业专项项目（201105007）; 宁波大学学科项目（XKZSC1503）. 第一作者: 刘晓琳（1991－）, 女, 安徽安庆人, 在读硕士研究生, 主要研究方向: 食品微生物学. E-mail: 1412649782@qq.com *通信作者: 金珊（1964－）, 女, 浙江天台人, 教授, 主要研究方向: 水产动物病害防控. E-mail: jinshan@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
