电针对脑卒中后抑郁大鼠谷氨酸类神经递质及其受体的表达影响
PDF下载 (22384)严嘉宁,赵立峰,余 涛,陆方琴,傅润熹,韦登明*.电针对脑卒中后抑郁大鼠谷氨酸类神经递质及其受体的表达影响[J].宁波大学学报(理工版),2018,31(3):72-75.DOI:
YAN Jia-ning,ZHAO Li-feng,YU Tao,LU Fang-qin,FU Run-xi,WEI Deng-ming*.Effects of electroacupuncture on glutamate (Glu) and N-methyl-D-aspartate receptor ( NMDAR) in hippocampal of PSD rats[J].Journal of Ningbo University(Natural Science & Engineering Edition),2018,31(3):72-75.DOI:
| Title: | Effects of electroacupuncture on glutamate (Glu) and N-methyl-D-aspartate receptor ( NMDAR) in hippocampal of PSD rats |
| 作者: | 严嘉宁, 赵立峰, 余 涛, 陆方琴, 傅润熹, 韦登明* |
| Author(s): | YAN Jia-ning, ZHAO Li-feng, YU Tao, LU Fang-qin, FU Run-xi, WEI Deng-ming* |
| 关键词: | 脑卒中后抑郁; 电针; 谷氨酸 |
| Keywords: | NMDA; AMPA; post-stroked depression (PSD); electroacupuncture (EA); glutamate (Glu); N-methyl-D-aspartate receptor (NMDAR); -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) |
| 分类号: | R395 |
| 文献标识码: | A |
| 摘要: | 观察电针对脑卒中后抑郁(PSD)大鼠的脑部氨基酸类神经递质表达的影响, 探讨电针治疗脑卒中后抑郁的分子机理. 将Wistar大鼠随机分为正常组、假手术组、模型组和电针治疗组, 采用夹闭颈总动脉和再通方法建立脑卒中模型. 7d后根据文献[1]方法建立PSD模型, 电针“百会”“大椎”穴, 留针20min, 1次·d-1共持续15d. 旷场试验观察其行为学表现, 免疫组织化学技术检测大鼠脑谷氨酸类神经递质的表达变化结果. 与模型组相比, 行为学实验发现电针治疗组大鼠旷场穿线抬壁次数明显增多, 差异具有统计学意义(P<0.05); 免疫组化检测发现谷氨酸及其受体NMDA/AMPA积分光密度值明显下降, 差异具有统计学意义(P<0.01). 表明电针可抑制脑卒中后抑郁大鼠脑NMDA/AMPA的表达, 这可能是电针治疗脑卒中后抑郁的分子机理之一. |
| Abstract: | The current study was aimed to determine the effect of electroacupuncture (EA) on glutamate (Glu) and N-methyl-D-aspartate receptor (NMDAR) levels in rats with post-stroke depression (PSD). Wistar rats were randomly divided into control, sham, PSD and EA+PSD groups. PSD rat model was established by repeated ischemia/reperfusion and pretreated with sodium nitroprusside. One 20 min session of EA was daily applied to “Baihui”(GV20), “Dazhui”(DU14) in EA group for 15 days. OPEN-FIELD test was performed to detect the rats’ locomotion. Glu, AMPAR and NMDAR produced in hippocampal tissue were examined with immunohisto- chemical assay. Compared with PSD group, both target-platform crossing and lifting times were significantly increased in EA group (P<0.05). Glu, AMPAR and NMDAR positive cells were remarkably decreased in EA-treated rats (P<0.01). It was therefore concluded that EA enabled to decrease the expressions of Glu, AMPAR and NMDAR in PSD rats. |
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| 备注/Memo: | 收稿日期: 2017?05?28. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 宁波市重大精神疾病转化医学研究项目（2015C110026）. 第一作者: 严嘉宁（1996－）, 男, 浙江嘉兴人, 在读硕士研究生, 主要研究方向: 脑缺血性疾病防治分子机制. E-mail: 419503527@qq.com *通信作者: 韦登明（1963－）, 男, 贵州贵定人, 博士/教授, 主要研究方向: 脑缺血性疾病防治分子机制. E-mail: 1760947688@qq.com 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
