低盐胁迫下三疣梭子蟹γ-氨基丁酸及其A型受体相关蛋白对渗透压调节的影响
PDF下载 (395)张伟佳,卢少坤,李荣华,王春琳,母昌考,宋微微.低盐胁迫下三疣梭子蟹γ-氨基丁酸及其A型受体相关蛋白对渗透压调节的影响[J].宁波大学学报(理工版),2019,32(3):9-14.DOI:
ZHANG Wei-jia,LU Shao-kun,LI Rong-hua*,WANG Chun-lin,MU Chang-kao,SONG Wei-wei.Effects of gamma-aminobutyric acid and GABAA receptor associated protein on osmotic regulation in Portunus trituberculatus under low salinity stress[J].Journal of Ningbo University(Natural Science & Engineering Edition),2019,32(3):9-14.DOI:
| Title: | Effects of gamma-aminobutyric acid and GABAA receptor associated protein on osmotic regulation in Portunus trituberculatus under low salinity stress |
| 作者: | 张伟佳, 卢少坤, 李荣华, 王春琳, 母昌考, 宋微微 |
| Author(s): | ZHANG Wei-jia, LU Shao-kun, LI Rong-hua*, WANG Chun-lin, MU Chang-kao, SONG Wei-wei |
| 关键词: | 三疣梭子蟹 ; 低盐胁迫; γ-氨基丁酸; γ-氨基丁酸A型受体相关蛋白 |
| Keywords: | Portunu strituberculatus; low salinity stress; gamma-aminobutyric acid (GABA); GABAA receptor associated protein (GABARAP) |
| 分类号: | Q592.1; S968.25 |
| 文献标识码: | A |
| 摘要: | 为探究低盐胁迫下γ-氨基丁酸(GABA)及其A型受体相关蛋白(GABARAP)在三疣梭子蟹渗透压调节过程中起到的作用, 实验检测了低盐度10条件下, 三疣梭子蟹在0, 3, 6, 9, 12, 24, 36, 48h的血清渗透压、血清氯离子(Cl-)浓度等指标, 同时对鳃组织的GABA量以及GABARAP基因表达量变化进行测定. 结果显示, 血清渗透压和血清Cl-浓度在12h内显著下降(P＜0.05), 12h后趋于稳定; 相对0h, 血清Cl-浓度降低17.93%, 血清渗透压降低24.46%, 与外界海水渗透压的差值维持在(183.21±9.51)mOsm·kg-1. GABA量及GABARAP基因表达量波动上升, 12h后基本维持稳定. 研究结果表明, 三疣梭子蟹对低盐度条件的适应性调节在12h内进行, 之后基本维持稳定, GABA量以及GABARAP基因表达量在此过程中呈现显著的正相关关系, 对血清Cl-浓度的调节起到了重要作用, 使得12h后血清渗透压与外界海水渗透压维持在(183.21±9.51)mOsm·kg-1, 保证各项生理活动的正常进行. |
| Abstract: | In order to investigate the effects of gamma-aminobutyric acid (GABA) and GABAA receptor associated protein (GABARAP) on osmotic regulation in Portunus trituberculatus under low salinity stress, the osmotic pressure and concentration of chloride ion (Cl-) in the serum of P. trituberculatus at 0, 3, 6, 9, 12, 24, 36 and 48 h were measured under salinity 10, the changes of GABA content and GABARAP gene expression in gill tissue were also determined at each time point. The results showed that osmotic pressure and concentration of chloride ion (Cl-) in the serum decreased significantly within 12 hours (P<0.05), and stabilized after 12 hours, the concentration of Cl- decreased 17.93% and the osmotic pressure of the serum decreased 24.46% compared to time 0 h, the difference between the serum osmotic pressure and the seawater was (183.21±9.51)mOsm·kg-1 after 12 h. The content of GABA and the gene expression of GABARAP increased at the beginning and became stable after 12h. These results indicated that the regulation of P. trituberculatus to low salinity conditions should be completed within 12h. GABA content and GABARAP gene expression showed a significant positive correlation during this process. The results suggest that they played an important role in the regulation of Cl- to maintain the difference between the serum osmotic pressure and the seawater at (183.21±9.51)mOsm·kg-1, which is of great importance for P. trituberculatus to ensure the normal physiological activities. |
| 参考文献 /References: | [1].周双林, 姜乃澄, 卢建平, 等. 甲壳动物渗透压调节的研究进展Ⅰ. 鳃的结构与功能及其影响因子[J]. 海洋学研究, 2001, 19(1):45-52. [2].王冲, 姜令绪, 王仁杰, 等. 盐度骤变和渐变对三疣梭子蟹幼蟹发育和摄食的影响[J]. 水产科学, 2010, 29(9): 510-514. [3].孙颖民, 宋志乐, 严瑞深, 等. 三疣梭子蟹生长的初步研究[J]. 生态学报, 1984, 4(1):59-66. [4].江山, 许强华. 盐度胁迫对三疣梭子蟹鳃Na+/K+- ATPase酶活的影响[J]. 水产学报, 2011, 35(10):1475- 1480. [5].堀江典子, 菅美奈子, 金武祚. GABA(γ-氨基丁酸)的功能性[J]. 中国食品添加剂, 2010(6):169-173. [6].AL-Quraan N A, Sartawe F A, Qaryouti M M. Characterization of γ-aminobutyric acid metabolism and oxidative damage in wheat (Triticum aestivum L.) seedlings under salt and osmotic stress[J]. Journal of Plant Physiology, 2013, 170(11):1003-1009. [7].Duci? I, Caruncho H J, Zhu W J, et al. gamma- Aminobutyric acid gating of Cl- channels in recombinant GABAA receptors[J]. Journal of Pharmacology & Experimental Therapeutics, 1995, 272(1):438- 445. [8].袁莲芳, 陈果, 贾宏阁, 等. GABAA受体研究现状[J]. 现代临床医学, 2014, 40(2):89-92. [9].Leil T A, Chen Z W, Chang C S, et al. GABAA receptor- associated protein traffics GABAA receptors to the plasma membrane in neurons. The Journal of Neuro- science: The Official Journal of the Society for Neuro- science, 2004, 24(50):11429-11438. [10].Chen Z W, Olsen R W. GABAA receptor associated proteins: A key factor regulating GABAA receptor function[J]. Journal of Neurochemistry, 2007, 100:279- 294. [11].Lima A G, Mcnamara J C, Terra W R. Regulation of hemolymph osmolytes and gill Na+/K+-ATPase activities during acclimation to saline media in the freshwater shrimp Macrobrachium olfersii (Wiegmann, 1836) (Decapoda, Palaemonidae)[J]. Journal of Experimental Marine Biology & Ecology, 1997, 215(1):81-91. [12].Via J D. The effect of salinity on free amino acids in the prawn Palaemon elegans (Rathke)[J]. Archiv Fur Hydrobiologie, 1989, 115(1):125-135. [13].Ren H, Li J, Li J, et al. Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress[J]. Acta Oceanologica Sinica, 2015, 34(8):52-61. [14].孙武卫. 低盐胁迫下凡纳滨对虾消减cDNA文库构建及谷氨酰胺合成酶cDNA克隆和表达[D]. 湛江: 广东海洋大学, 2012. [15].Morris S. Neuroendocrine regulation of osmoregulation and the evolution of air-breathing in decapod crustaceans [J]. Journal of Experimental Biology, 2001, 204(5):979- 989. [16].Chen J C, Chia P G. Osmotic and ionic concentrations of Scylla serrata subjected to different salinity levels[J]. Comparative Biochemistry & Physiology Part A Physiology, 1997, 117(2):239-244. [17].马金武, 吕建建, 刘萍, 等. 急性盐度胁迫对三疣梭子蟹(Portunus trituberculatus)黄选1号血清渗透压及离子含量的影响[J]. 渔业科学进展, 2016, 37(1):58-62. [18].王林, 潘鲁青. 三疣梭子蟹在低盐胁迫下渗透生理适应性的研究[J]. 海洋湖沼通报, 2016(3):106-112. [19].卢俊, 庄平, 冯广朋, 等. 中华绒螯蟹亲蟹渗透压调节和抗氧化系统对盐度的响应[J]. 海洋渔业, 2011, 33(1): 39-45. [20].马金武, 吕建建, 刘萍, 等. 盐度胁迫对三疣梭子蟹黄选号血清部分生化指标的影响[J]. 水产学报, 2017, 41(4):506-511. [21].王秀坤, Nielsen M. GABAA受体药理学研究进展[J]. 国际药学研究杂志, 2001, 28(1):29-34. [22].梁臣陈忠. γ-氨基丁酸及其受体功能的研究与应用现状[J]. 动物医学进展, 2015, 36(4):108-112. [23].付萍吕建建刘萍等. 盐度胁迫对三疣梭子蟹肌肉和血淋巴中游离氨基酸含量的影响[J]. 水产学报2017, 41(3):374-381. [24].Eshaq R S, Stahl L D, Smith S S, et al. GABA acts as a ligand chaperone in the early secretory pathway to promote cell surface expression of GABAA receptors[J]. Brain Research, 2010, 1346(8):1-13. |
| 备注/Memo: | 收稿日期: 2018?09?13. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（41476124）; 浙江省自然科学基金（LY17C190005）; 宁波市科技计划（2017C110007, 2016C10037）. 第一作者: 张伟佳（1994－）, 男, 浙江平湖人, 在读硕士研究生, 主要研究方向: 水产 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
