纳米复合载体固定酪氨酸酶合成左旋多巴
PDF下载 (293)汪定林,张瑞丰.纳米复合载体固定酪氨酸酶合成左旋多巴[J].宁波大学学报(理工版),2023,36(4):33-41.DOI:10.20098/j.cnki.1001-5132.2022.1023
WANG Dinglin,ZHANG Ruifeng.Synthesis of L-DOPA by tyrosinase immobilized on nano-composite carriers[J].Journal of Ningbo University(Natural Science & Engineering Edition),2023,36(4):33-41.DOI:10.20098/j.cnki.1001-5132.2022.1023
| Title: | Synthesis of L-DOPA by tyrosinase immobilized on nano-composite carriers |
| 作者: | 汪定林, 张瑞丰 |
| Author(s): | WANG Dinglin, ZHANG Ruifeng |
| 关键词: | 纳米复合材料; 酪氨酸酶; 固定化酶; 左旋多巴 |
| Keywords: | nano-composites; tyrosinase; immobilized enzyme; L-DOPA |
| 分类号: | TQ464.8 |
| DOI: | 10.20098/j.cnki.1001-5132.2022.1023 |
| 文献标识码: | A |
| 摘要: | 利用模板法制备大孔SiO2材料, 而后借助水热合成法在孔道中生长ZnO纳米线, 得到新型纳米复合材料(SiO2/ZnO NWs). 采用静电吸附法将酪氨酸酶(TYR)固定在纳米复合载体上, 用于L-多巴合成. 从扫描电子显微镜中观察到ZnO纳米线在孔道中呈现无规线团形貌, 且分布均匀. TYR在SiO2/ZnO NWs的最大负载量高达162.3mg?g-1, 约是纯大孔SiO2载体3倍, 且远高于其他固定化TYR系统. 利用固定化TYR进行L-多巴合成, 在最优反应条件(35℃、pH6.0、L-抗坏血酸浓度10 mmol?L-1)下催化1.5h, L-多巴的产率可达70.2％. 固定化TYR展现良好的储存稳定性, 储存28d仍保持78.6％的相对活性, 远高于游离状态TYR, 并可重复使用, 经历10个循环后仍能保持42.1％L-多巴产率. |
| Abstract: | The macroporous SiO2 material is prepared using the template method, while ZnO nanowires are grown in the pores assisted using hydrothermal synthesis method to obtain new nano-composites (SiO2/ZnO NWs). TYR is effectively immobilized on nano-composited support by electrostatic adsorption and used for the synthesis of L-DOPA. From the scanning electron microscopy (SEM), we observe that the ZnO nanowires show a random wire cluster morphology with uniform distribution in the pores. The maximum loading of TYR on SiO2/ZnO NWs is up to 162.3 mg?g-1, which is approximately three times more than the pure macroporous SiO2 support and much higher than other immobilized TYR systems. The immobilized TYR is used for the synthesis of L-DOPA. The optimized reaction conditions are identified and the yield of L-DOPA reaches 70.2％ in 1.5 h of catalysis under these conditions. The immobilized TYR also shows good storage stability, maintaining 78.6％ relative activity after 28 days of storage, which is also much higher than that of the free TYR. As for the reusability, the L-DOPA yield of 42.1％ can be maintained after 10 cycles. |
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| 备注/Memo: | 收稿日期: 2022－10－31. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 浙江省自然科学基金（LY15B010002）.第一作者: 汪定林（1997－）, 男, 安徽安庆人, 在读硕士研究生, 主要研究方向: 固定化酶. E-mail: 1538726613@qq.com*通信作者: 张瑞丰（1967－）, 男, 浙江宁波人, 研究员, 主要研究方向: 无机多孔材料及其纳米复合与功能化. E-mail: zhangruifeng@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
