氨基磁性纳米粒子对α-胰凝乳蛋白酶固定过程的研究
PDF下载 (375)李 静1,钟国伦2,夏海平3*.氨基磁性纳米粒子对α-胰凝乳蛋白酶固定过程的研究[J].宁波大学学报(理工版),2016,29(1):77-81.DOI:
LI Jing1,ZHONG Guo-lun2,XIA Hai-ping3*.Process of -chymotrypsin Immobilized on Amino-functionalized Magnetic Nanoparticles: Research[J].Journal of Ningbo University(Natural Science & Engineering Edition),2016,29(1):77-81.DOI:
| Title: | Process of -chymotrypsin Immobilized on Amino-functionalized Magnetic Nanoparticles: Research |
| 作者: | 李 静1, 钟国伦2, 夏海平3* |
| Author(s): | LI Jing1, ZHONG Guo-lun2, XIA Hai-ping3* |
| 关键词: | 氨基功能化磁性纳米粒子; α-胰凝乳蛋白酶; 载酶量; 等温式; 动力学 |
| Keywords: | amino-functionalized magnetic nanoparticles; -chymotrypsin; binding capacity; isotherm; kinetic |
| 分类号: | O648.1 |
| 文献标识码: | A |
| 摘要: | 采用分子自组装技术制备氨基功能化磁性纳米粒子, 并通过碳二亚胺偶联剂将α-胰凝乳蛋白酶固定在其表面. 利用红外光谱及热失重分析等方法表征固定酶的纳米粒子, 研究了固定过程中酶浓度和反应时间对载酶量的影响, 得到最佳固定条件. 在固定过程用Langmuir等温式拟合优于用Freundlich等温式, 功能化纳米粒子的载酶量达到127mg?g-1, 与一级反应动力学模型相比, 二级反应动力学模型能够更好地拟合该固定过程. |
| Abstract: | In this paper, a novel amino-functionalized Fe3O4 nanoparticles is presented by molecular self-assembly and bound to α-chymotrypsin in presence of carbodiimide. The immobilized α-chymotrypsin NPs are characterized by IR and TGA. The effect of parameters such as the initial concentration and reaction time on the process of immobilization is investigated. The Langmuir isotherm model is in agreement with the experimental data better than Freundlich isotherm model in the process of immobilization. The binding capacity of functional nanoparticles is measured for being 127 mg·g-1. The immobilization kinetic data correlate better with the second order equation than with the first order equation |
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| 备注/Memo: | 收稿日期: 2014?02?28. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 浙江省自然科学基金（ LY12B03002 ） . 第一作者: 李静（1987－）, 男, 江苏淮安人, 在读硕士研究生, 主要研究方向: 磁性纳米材料. E-mail: salvationhope@163.com * 通信作者: 夏海平（ 1967 －） , 男 , 浙江舟山人 , 博士 / 研究员 , 主要研究方向 : 功能材料 . E-mail: hpxcm@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
