一株海杆菌的溶藻活性及基于基因组的溶藻机理分析
PDF下载 (365)吴寒华1,2,李登峰1,2*,严小军1,2,徐 然1,刘 飞1,周成旭1,刘联国1.一株海杆菌的溶藻活性及基于基因组的溶藻机理分析[J].宁波大学学报(理工版),2017,30(1):23-28.DOI:
WU Han-hua1,2,LI Deng-feng1,2*,YAN Xiao-jun1,2,XU Ran1,LIU Fei1,ZHOU Cheng-xu1,LIU Lian-guo1.Algicidal activity of Marinobacter sp. YWL01 and algicidal mechanism analysis based on its genome sequence[J].Journal of Ningbo University(Natural Science & Engineering Edition),2017,30(1):23-28.DOI:
| Title: | Algicidal activity of Marinobacter sp. YWL01 and algicidal mechanism analysis based on its genome sequence |
| 作者: | 吴寒华1, 2, 李登峰1, 2*, 严小军1, 2, 徐 然1, 刘 飞1, 周成旭1, 刘联国1 |
| Author(s): | WU Han-hua1, 2, LI Deng-feng1, 2*, YAN Xiao-jun1, 2, XU Ran1, LIU Fei1, ZHOU Cheng-xu1, LIU Lian-guo1 |
| 关键词: | 海杆菌; 中肋骨条藻; 溶藻; 基因组; 赤潮 |
| Keywords: | Marinobacter; Skeletonema costatum; algicidal; genome sequence; algal blooming |
| 分类号: | Q89 |
| 文献标识码: | A |
| 摘要: | 为了解新型海杆菌Marinobacter)菌株YWL01溶藻活性及其可能的溶藻机理YWL01与常见的赤潮藻中肋骨条藻Skeletonema costatum)混合培养, 通过肉眼、光电镜观察及藻叶绿素a浓度测定来评估溶藻活性采用Illumina Miseq测序技术测定YWL01基因组将预测基因的蛋白序列分别进行BLASTP比对注释信息分析其可能的溶藻机理结果表明YWL01可直接溶藻藻液颜色由黄褐色逐渐变淡藻细胞裂解藻叶绿素a浓度明显降 低YWL01基因组中许多与溶藻相关的基因 |
| Abstract: | To explore the algicidal activity and mechanism of a novel Marinobacter sp. YWL01, Skeletonema costatum cultures are added with YWL01. Cell condition and signs of lysis in each algal culture are monitored with a variety of means including visual inspection, optical microscopy, transmission electron microscopy. Genomic DNA of YWL01 is sequenced with Illumina Miseq. Functional annotations are made in a bid to analyze the algicidal mechanism through a BLASTP search. The results reveal that YWL01 exhibits algicidal activity through a direct attack. After the inoculation of YWL01 cells, the color of the S. costatum cultures turn from brown to pale, and the cytoplasmic and photosynthetic pigments are found to be significantly degraded compared to those in healthy cells. The lysate has a statistically significant impact on the chl-a content of S. costatum. The genome of YWL01 contains genes about algicidal mechanism. The work presented in this report may facilitate understanding the genetic underpinning of algae-bacterial interactions, and is expected to be useful for further studies of algal blooming. |
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| 备注/Memo: | 收稿日期: 2016?03?19. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家科技部星火计划（2014GA701018）; 宁波市科技局农业攻关项目（2011C10004）; 浙江省教育厅科研项目（Y201327815）; 宁波市海洋蟹类产业科技创新团队项目（2011B81003）; 浙江省重点学科开放基金（E02009124200）; 宁波大学科研项目（xkzsc1519）; 宁波大学王宽诚幸福基金. 第一作者: 吴寒华（1990－）, 女, 浙江绍兴人, 在读硕士研究生, 主要研究方向: 水生生物细菌和病毒. E-mail: 497651554@qq.com *通信作者: 李登峰（1975－）, 女, 湖南双峰人, 副教授, 主要研究方向: 水产动物病害防治. E-mail: lidengfeng@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
