螺旋藻肽对四氧嘧啶诱导的糖尿病小鼠作用研究
PDF下载 (330)陈雨豪,王 峰,周嘉威,牛婷婷,宣荣荣,吴 玮,陈海敏.螺旋藻肽对四氧嘧啶诱导的糖尿病小鼠作用研究[J].宁波大学学报(理工版),2020,33(3):13-18.DOI:
CHEN Yuhao,WANG Feng,ZHOU Jiawei,NIU Tingting,XUAN Rongrong,WU Wei,CHEN Haimin.The effects of Spirulina peptides on alloxan-induced diabetic mice[J].Journal of Ningbo University(Natural Science & Engineering Edition),2020,33(3):13-18.DOI:
| Title: | The effects of Spirulina peptides on alloxan-induced diabetic mice |
| 作者: | 陈雨豪, 王 峰, 周嘉威, 牛婷婷, 宣荣荣, 吴 玮, 陈海敏 |
| Author(s): | CHEN Yuhao, WANG Feng, ZHOU Jiawei, NIU Tingting, XUAN Rongrong, WU Wei, CHEN Haimin |
| 关键词: | 螺旋藻; 多肽; 糖尿病; 降血糖 |
| Keywords: | Spirulina; peptides; diabetes; hypoglycemic |
| 分类号: | R151 |
| 文献标识码: | A |
| 摘要: | 为了评价螺旋藻肽的降血糖活性及对糖尿病小鼠的降糖效果, 采用腹腔注射四氧嘧啶构建2型糖尿病小鼠模型. 实验设对照组、模型组、二甲双胍阳性对照组以及低、中、高3种浓度螺旋藻肽组, 连续喂养糖尿病小鼠21d后检测体重、食物和水消耗量、葡萄糖耐量、血糖和脂代谢相关指标、血清尿素氮、肌酸酐, 观察分析糖尿病小鼠胰腺组织. 结果表明 与模型组相比 螺旋藻肽组有降小鼠血糖和体重作用, 可减少小鼠食物和水的消耗量, 显著改善小鼠葡萄糖耐量; 高剂量的螺旋藻肽组比模型组显著减少了小鼠血清中的总胆固醇、糖基化血清蛋白, 增加了血清胰岛素和肝脏肝糖原含量, 小鼠的胰腺组织明显好转. 研究表明螺旋藻肽具有降血糖活性, 其抗糖尿病作用可能与改善胰岛素分泌功能和调节糖脂代谢有关. |
| Abstract: | To evaluate the hypoglycemic activity of spirulina and its effect on diabetes, intraperitoneal injection of alloxan was used to construct a mouse model of type 2 diabetes. The control group, metformin group, low-, medium-, and high-dose spirulina peptides groups were used in the current studies. Following 21 days of feeding, body weight, food and water consumption, glucose tolerance, blood glucose metabolism and lipid metabolism related indicators, serum urea nitrogen, and creatinine were measured. In addition, pancreatic tissues were collected and pathological analysis was performed. Compared with the control group, spirulina peptides showed a hypoglycemic effect and significantly reduced the body weight of diabetic mice, food and water consumption. Compared with the control group, the treatment of spirulina peptides significantly improved the glucose tolerance. In contrast to the control group, high-dose spirulina peptides significantly reduced total cholesterol and glycosylated serum protein, increased serum insulin and liver glycogen content, and significantly improved the pancreatic morphology of diabetic mice. The present studies showed that spirulina peptides have hypoglycemic activity which may be related to improving insulin secretion and glucose/lipid metabolism. |
| 参考文献 /References: | [1] 刘盈盈, 蔡云汐, 王淑娥, 等. 藜麦对糖尿病模型小鼠降糖效果研究[J]. 营养学报, 2019, 41(3):261-264. [2] Patil P, Mandal S, Tomar S K, et al. Food protein-derived bioactive peptides in management of type 2 diabetes[J]. European Journal of Nutrition, 2015, 54(6):863-880. [3] Ou Y, Lin L, Yang X G, et al. Antidiabetic potential of phycocyanin: Effects on KKAy mice[J]. Pharmaceutical Biology, 2013, 51(5):539-544. [4] Chaudhury A, Duvoor C, Reddy Dendi V S, et al. Clinical review of antidiabetic drugs: Implications for type 2 diabetes mellitus management[EB/OL]. [2019-12-10]. https:// www.frontiersin.org/articles/10.3389/fendo.2017.00006/full. [5] Wang Z J, Zhang X W. Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis[J]. Journal of the Science of Food and Agriculture, 2017, 97(3):918-922. [6] Sun Y J, Chang R, Li Q Y, et al. Isolation and characterization of an antibacterial peptide from protein hydrolysates of Spirulina platensis[J]. European Food Research and Technology, 2016, 242(5):685-692. [7] 余颉, 董秋燕. 螺旋藻在饲料中的应用研究进展[J]. 绿色科技, 2018(23):51-53. [8] Lu J, Ren D F, Xue Y L, et al. Isolation of an antihypertensive peptide from alcalase digest of Spirulina platensis[J]. Journal of Agricultural and Food Chemistry, 2010, 58(12):7166-7171. [9] Fan X D, Cui Y J, Zhang R L, et al. Purification and identification of anti-obesity peptides derived from Spirulina platensis[J]. Journal of Functional Foods, 2018, 47:350-360. [10] Yu J, Hu Y, Xue M, et al. Purification and identification of antioxidant peptides from enzymatic hydrolysate of Spirulina platensis[J]. Journal of Microbiology and Biotechnology, 2016, 26(7):1216-1223. [11] 淤泽涛, 林青, 孙创斌, 等. 去脂软肝丸对四氧嘧啶所致小鼠糖尿病性高脂血症的影响[J]. 中国药理通讯, 2003(2):44. [12] Shin S O, Seo H J, Park H, et al. Effects of mulberry leaf extract on blood glucose and serum lipid profiles in patients with type 2 diabetes mellitus: A systematic review[J]. European Journal of Integrative Medicine, 2016, 8(5):602-608. [13] 吴晓瑜. 二甲双胍治疗高脂血症临床观察[J]. 现代医药卫生, 2009, 25(14):2131. [14] 王波, 刘衡川, 洪君蓉, 等. 番石榴叶水提取物对糖尿病小鼠小肠α-葡萄糖苷酶活性的影响[J]. 四川大学学报(医学版), 2007, 38(2):298-301. [15] 唐健元, 马莉. 糖化血红蛋白和糖化血清蛋白在糖尿病中药新药临床研究设计中的作用[J]. 中药新药与临床药理, 2007, 18(2):162-164. [16] Fernández-Millán E, Cordero-Herrera I, Ramos S, et al. Cocoa-rich diet attenuates beta cell mass loss and function in young Zucker diabetic fatty rats by preventing oxidative stress and beta cell apoptosis[J]. Molecular Nutrition & Food Research, 2015, 59(4):820-824. [17] 吕娟, 魏鹏飞, 白甫. 山药多糖对2型糖尿病大鼠血小板及酶活性的影响[J]. 中国老年学杂志, 2017, 37(13): 3186-3187. [18] Adeva-Andany M M, González-Lucán M, Donapetry- García C, et al. Glycogen metabolism in humans[J]. BBA Clinical, 2016, 5:85-100. [19] Saravanan G, Ponmurugan P, Deepa M A, et al. Modulatory effects of diosgenin on attenuating the key enzymes activities of carbohydrate metabolism and glycogen content in streptozotocin-induced diabetic rats [J]. Canadian Journal of Diabetes, 2014, 38(6):409-414. [20] 王茵, 吴成业, 刘淑集, 等. 坛紫菜酶解产物对糖尿病模型小鼠的血糖调节作用[J]. 渔业科学进展, 2013, 34(4):118-124. [21] Almdal T P, Vilstrup H. Strict insulin therapy normalises organ nitrogen contents and the capacity of urea nitrogen synthesis in experimental diabetes in rats[J]. Diabetologia, 1988, 31(2):114-118. [22] Alam M M, Meerza D, Naseem I. Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice[J]. Life Sciences, 2014, 109(1):8-14. |
| 备注/Memo: | 收稿日期:2020-01-12.宁波大学学报（理工版）网址:http://journallg.nbu.edu.cn/ 基金项目:国家自然科学基金（31872540）;浙江省自然科学基金（LY19H030001,LY18C190004）. 第一作者:陈雨豪（1995－）,男,浙江宁波人,在读硕士研究生,主要研究方向:海洋生物.E-mail:467899653@qq.com *通信作者:陈海敏（1975－）,女,浙江瑞安人,研究员,主要研究方向:海洋生物.E-mail:chenhaimin@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
