银鲳脾脏和头肾的形态与组织学特征
PDF下载 (7148)季益平,匡思雯,袁 子,王亚军,徐善良,唐道军.银鲳脾脏和头肾的形态与组织学特征[J].宁波大学学报(理工版),2020,33(4):1-5.DOI:
JI Yiping,KUANG Siwen,YUAN Zi,WANG Yajun,XU Shanliang,TANG Daojun.Morphological and histological characteristics of spleen and head-kidney of Pampus argenteus[J].Journal of Ningbo University(Natural Science & Engineering Edition),2020,33(4):1-5.DOI:
| Title: | Morphological and histological characteristics of spleen and head-kidney of Pampus argenteus |
| 作者: | 季益平, 匡思雯, 袁 子, 王亚军, 徐善良, 唐道军 |
| Author(s): | JI Yiping, KUANG Siwen, YUAN Zi, WANG Yajun, XU Shanliang, TANG Daojun |
| 关键词: | 银鲳; 脾脏; 头肾; 形态特征; 组织结构 |
| Keywords: | Pampus argenteus; spleen; head-kidney; morphological characteristics; tissue structure |
| 分类号: | S965 |
| 文献标识码: | A |
| 摘要: | 为了解银鲳(Pampus argenteus)免疫器官的特点, 采用解剖及组织切片显微技术, 对银鲳脾脏和头肾的形态与组织学结构特征进行观察. 结果显示: 银鲳的脾脏位于胃后方, 前肠背侧, 外被薄层结缔组织, 内部实质由红髓和白髓组成; 脾小梁结构不明显, 内含大量淋巴细胞、红细胞及丰富的动脉、静脉和椭圆体结构; 有黑色素巨噬细胞及其聚集形成的黑色素巨噬细胞中心, 无神经束结构; 头肾位于食道背侧, 口腔内咽退缩肌上方, 表面有胶原纤维构成的薄层被膜, 内部为淋巴样实质组织, 无肾单位结构; 头肾含有丰富的淋巴细胞和少量红细胞, 分布有动脉、静脉和毛细血管, 在毛细血管旁存在肾上组织, 实质中可见黑色素巨噬细胞中心. 研究结果表明, 银鲳的脾脏和头肾是体内重要的免疫和造血器官. |
| Abstract: | In order to understand the characteristics of the immune organs of Pampus argenteus, the current study used the dissect and tissue slice microtechnic, and observed the microstructure of the spleen and head kidney of Pampus argenteus. The results showed that the spleen lied behind the stomach and the back of the foregut. It was covered with a thin layer of connective tissue. Spleen parenchyma was formed by alternating arrangement of red and white pulp. The spleen trabecular structure was not evident. There were a large number of lymphocytes and blood cells in the tissue. The cells were rich in artery, vein and ellipsoid structure. The cells were present with melanin macrophages without nerve bundle structure. The head-kidney of Pampus argenteus was located at the back of esophagus and above the constrictor naris in oral cavity. They were surrounded by collagen fiber structure which belonged to lymphoid parenchyma without nephron structure. The head-kidney was rich in lymphocytes, with a small amount of blood cells. The distribution of arteries, veins and capillaries was also observed. The adrenal tissue existed by the capillaries side. Melano-macrophage center was visible in the parenchyma. In conclusion, the spleen and head-kidney are the important immune organs and hematopoietic organs of Pampus argenteus. |
| 参考文献 /References: | [1] Dalmo R A, Ingebrigtsen K, Bogwald J. Non-specific defence mechanisms in fish, with particular reference to the reticuloendothelial system (RES)[J]. Journal of Fish Diseases, 1997, 20(4):241-273. [2] 李长玲, 曹伏君, 刘楚吾, 等. 花尾胡椒鲷脾脏和头肾显微结构的观察[J]. 海洋通报, 2002, 21(2):30-35. [3] 胡玲玲, 李加儿, 区又君, 等. 条石鲷头肾和脾脏的显微结构观察[J]. 南方水产, 2010, 6(3):41-45. [4] 秦国强, 王浩, 黄金生, 等. 鲤鱼和鲫鱼脾脏显微结构和亚显微结构的研究[J]. 水生生物学集刊, 1983, 7(1):63-70. [5] 王鸿鹄, 李长玲, 曹伏君, 等. 红笛鲷头肾和脾脏显微结构的观察[J]. 台湾海峡, 2006, 25(3):360-367. [6] 曹守花, 李加儿, 区又君, 等. 鲻脾脏和头肾的形态学与组织学研究[J]. 海洋渔业, 2013, 35(3):249-256. [7] 罗俊标, 谢木娇, 区又君, 等. 四指马鲅头肾和脾脏组织学研究[J]. 生物学杂志, 2016, 33(4):43-47. [8] 区又君, 刘江华, 李加儿, 等. 驼背鲈头肾和脾脏的形态组织学观察[J]. 南方农业学报, 2015, 46(11):2034-2039. [9] 王腾飞, 张鼎元, 郭春阳, 等. 养殖银鲳性腺发育规律和性类固醇激素变化[J]. 水产学报, 2017, 41(2):198-211. [10] 徐善良, 王丹丽, 徐继林, 等. 东海银鲳(Pampus argenteus)、灰鲳(P. cinereus)和中国鲳(P. sinensis)肌肉主要营养成分分析与评价[J]. 海洋与湖沼, 2012, 43(4):775-782. [11] 朱晓芳, 郭晓鸽, 张鼎元, 等. 银鲳(Pampus argenteus)胰岛素样生长因子-Ⅰ基因的克隆和表达分析[J]. 海洋与湖沼, 2016, 47(6):1221-1232. [12] 谢碧文, 张未丽, 张耀光. 瓦氏黄颡鱼和岩原鲤脾脏的组织学观察[J]. 四川动物, 2010, 29(2):212-214; 333. [13] 祁保霞. 黄颡鱼脾脏和头肾的显微结构观察[J]. 内蒙古民族大学学报(自然科学版), 2010, 25(3):304-306. [14] 张训蒲, 熊传喜, 黎会平. 黄鳝造血器官的组织学观察[J]. 华中农业大学学报, 1993, 12(3):285-288; 306. [15] Santos A A, Gutierre R C, Antoniazzi M M, et al. Morphocytochemical, immunohistochemical and ultra-structural characterization of the head kidney of fat snook Centropomus parallelus[J]. Journal of Fish Biology, 2011, 79(7):1685-1707. [16] 田敬云, 谢海侠, 姚卫建, 等. 鳜鱼头肾的组织发生及成鱼头肾B淋巴细胞的分布[J]. 动物学报, 2005, 51(3):440-446. [17] 张孟闻. 脊椎动物的比较解剖学[M]. 北京: 高等教育出版社, 1998:104. [18] 苏锦祥. 鱼类学与海水鱼类养殖[M]. 2版. 北京: 中国农业出版社, 2005:88. |
| 备注/Memo: | 收稿日期: 2019－12－17. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 国家自然科学基金（31872586）; 浙江省重大科技专项（2019C02059）; 浙江省自然科学基金（LY18C190008）.第一作者: 季益平（1995－）, 男, 安徽芜湖人, 在读硕士研究生, 主要研究方向: 鱼类健康养殖. E-mail: 1643421349@qq.com*通信作者: 唐道军（1984－）, 男, 湖南永州人, 实验师, 主要研究方向: 水产养殖. E-mail: tangdaojun@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
