丝素蛋白激活Nrf2/Keap1/HO-1信号通路促进皮肤创面愈合
PDF下载 (43)刘 栋,刘俊杰,刘聪莹,曲 鹏,陶 然,杨利敏.丝素蛋白激活Nrf2/Keap1/HO-1信号通路促进皮肤创面愈合[J].宁波大学学报(理工版),2026,39(2):59-66.DOI:10.20098/j.cnki.1001-5132.2025.1008
LIU Dong,LIU Junjie,LIU Congying,QU Peng,TAO Ran,YANG Limin.Silk fibroin activates Nrf2/Keap1/HO-1 signaling pathway to promote skin wound healing[J].Journal of Ningbo University(Natural Science & Engineering Edition),2026,39(2):59-66.DOI:10.20098/j.cnki.1001-5132.2025.1008
| Title: | Silk fibroin activates Nrf2/Keap1/HO-1 signaling pathway to promote skin wound healing |
| 作者: | 刘 栋, 刘俊杰, 刘聪莹, 曲 鹏, 陶 然, 杨利敏 |
| Author(s): | LIU Dong, LIU Junjie, LIU Congying, QU Peng, TAO Ran, YANG Limin |
| 关键词: | 丝素蛋白; 创面愈合; 氧化应激; Nrf2/Keap1/HO-1信号通路 |
| Keywords: | silk fibroin; wound healing; oxidative stress; Nrf2/Keap1/HO-1 signalingpathway |
| 分类号: | R722.14+2；R966 |
| DOI: | 10.20098/j.cnki.1001-5132.2025.1008 |
| 文献标识码: | A |
| 摘要: | 探讨丝素蛋白通过调控核因子E2相关因子2（Nuclear factor erythroid 2-related factor 2，Nrf2）/Kelch样ECH关联蛋白1（Kelch-like ECH-associated protein 1, Keap1）/血红素加氧酶-1（Hemoglobin oxygenase-1，HO-1）信号通路促进皮肤创面愈合的作用机制。采用3~4周龄雌性SPF级BALB/c-nu裸小鼠，建立背部全皮层创面模型，随机分为正常对照组、凡士林组、5%丝素蛋白组、维生素E组及SP+Nrf2抑制剂Brusatol组，连续21d每日局部给药1次。第0、7、14、21天测量创面面积，第21天取皮H&E染色、Masson染色并检测Nrf2、Keap1、HO-1蛋白表达，第21天取血清检测总超氧化物歧化酶（Total Superoxide Dismutase，T-SOD）、丙二醛（Malonaldehyde，MDA）、过氧化氢酶（Catalase，CAT）含量。与对照组及凡士林组相比，5%丝素蛋白组能显著缩小创面面积，促进表皮及真皮结构修复和胶原增生，增加T-SOD及CAT并降低MDA水平，上调Nrf2、HO-1和下调Keap1蛋白表达（均P＜0.05），说明丝素蛋白可激活Nrf2/Keap1/HO-1信号通路和调节氧化应激促进皮肤创面愈合。 |
| Abstract: | This paper aims to investigate the mechanism by which silk fibroin promotes skin wound healing through regulation of Nrf2/Keap1/HO-1 signaling pathway. 3-4 week-old female SPF-grade BALB/c-nu nude mice were used to establish dorsal full-thickness skin wound models. Groups were randomly assigned as follows: normal control, vaseline, 5% silk fibroin, vitamin E and SP+Nrf2 inhibitor brusatol. Topical application was administered once daily for 21 consecutive days. Wound area measurements were taken on days 0, 7, 14, and 21. On day 21, skin samples were harvested for H&E staining, Masson’s trichrome staining, and detection of Nrf2, Keap1, HO-1 protein expression. On day 21, serum samples were collected to measure T-SOD, MDA and CAT levels. Compared with control and vaseline groups, the 5% silk fibroin group significantly reduced wound area, promoted epidermal and dermal structural repair and collagen proliferation, increased T-SOD and CAT levels while decreasing MDA levels, and upregulated Nrf2 and HO-1 protein expression while downregulating Keap1 expression (all P<0.05). The results demonstrated that silk fibroin activates Nrf2/Keap1/HO-1 signaling pathway and modulates oxidative stress to promote skin wound healing. |
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| 备注/Memo: | 收稿日期：2025-10-17 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ 基金项目：辽宁省教育厅科学研究项目（L2019602） 第一作者：刘 栋，硕士/主管药师，主要研究方向为神经生物学和药理学。E-mail: 845518225@qq.com *通信作者：杨利敏，博士/教授，主要研究方向为神经生物学和药理学。E-mail: yanglimin@dlu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
