纳米二氧化硅体内与体外氧化应激及细胞毒性实验研究
PDF下载 (543)史宏博 1,王 尊 1,于宏升 2,刘 魁 1,岳 霞 1,郑茹茹 1,邹宝波 1,赵进顺 1*.纳米二氧化硅体内与体外氧化应激及细胞毒性实验研究[J].宁波大学学报(理工版),2013,26(01):127-132.DOI:
SHI Hong-bo 1,WANG Zun 1,YU Hong-sheng 2,LIU Kui 1,YUE Xia 1,ZHENG Ru-ru 1,ZOU Bao-bo 1,ZHAO Jin-shun 1*.In Vitro and in Vivo Oxidative Stress and Cytotoxicity Induced by Nano-silica[J].Journal of Ningbo University(Natural Science & Engineering Edition),2013,26(01):127-132.DOI:
| Title: | In Vitro and in Vivo Oxidative Stress and Cytotoxicity Induced by Nano-silica |
| 作者: | 史宏博 1, 王 尊 1, 于宏升 2, 刘 魁 1, 岳 霞 1, 郑茹茹 1, 邹宝波 1, 赵进顺 1* |
| Author(s): | SHI Hong-bo 1, WANG Zun 1, YU Hong-sheng 2, LIU Kui 1, YUE Xia 1, ZHENG Ru-ru 1, ZOU Bao-bo 1, ZHAO Jin-shun 1* |
| 关键词: | 纳米二氧化硅; 小鼠; JB6细胞; 氧化应激; 细胞凋亡 |
| Keywords: | FAS; nano-silica; mice; JB6 cells; oxidative stress; cell apoptosis |
| 分类号: | R318 |
| 文献标识码: | A |
| 摘要: | 研究了纳米二氧化硅(SiO2)体内及体外氧化应激损伤及细胞毒性作用及机制. 体内实验采用ICR小鼠, 纳米SiO2经口灌胃染毒3次, 检测肺组织中MDA(丙二醛)和SOD(超氧化物歧化酶)含量, 观察纳米SiO2对小鼠肺的氧化应激毒性. 采用体外培养的小鼠上皮细胞(JB6细胞), 观察不同质量浓度纳米SiO2染毒后的细胞形态改变, 通过蛋白免疫印迹实验检测纳米SiO2是否通过FAS信号途径引起细胞凋亡. 研究结果表明: 纳米SiO2经口染毒后对小鼠肺具有氧化应激损伤作用, 表现为随染毒剂量的升高, 肺组织中MDA产生量增加, 呈一定的剂量－反应关系. 同时, 肺组织内SOD水平总体趋势降低, 但是差别未见统计学意义. 体外培养细胞实验中, 纳米SiO2染毒后可引起细胞出现凋亡并呈空泡样变. 蛋白免疫印迹实验中, 纳米SiO2可引起FAS信号蛋白表达量明显上升, 但是FADD表达水平并不升高. |
| Abstract: | To elucidate the mechanisms of nano-silica induced cytotoxicity and oxidative stress in vivo and in vitro. ICR mice were contaminated using nano-silica three times by oral gavage. The level of MDA (malondialdehyde) and SOD (superoxide dismutase) in the lung tissue were detected as in vivo toxicity endpoints for nano-silica induced oxidative stress. In vitro, mouse epithelial cells (JB6 cells) were cultured, and observed for morphologic changes after treatment with different concentrations of nano-silica. Induction of apoptosis by nano-silica via the FAS signaling pathway was examined by western-blot assay. The result shows that nano-silica induced oxidative stress in mice after oral exposure which is indicated by a significant increase of MDA in lung tissue in a dose-response manner. The SOD levels in lung tissue were decreased. Nano-silica caused oxidative damage in JB6 cells exposed to certain dose ranges. Cell apoptosis were also observed in cells stained with YO-PRO-1 dye. Western blot shows a significant increase in the expression of FAS signaling protein induced by nano-silica. However the level of FADD expression was decreased. |
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| 备注/Memo: | 收稿日期: 2012?07?01. 基金项目: 国家自然科学基金（81273111）; 宁波市自然科学基金（2012A610185）; 宁波大学新苗计划（2011R405035）.第一作者: 史宏博（1988－）, 女, 内蒙古呼伦贝尔人, 在读硕士研究生, 主要研究方向: 职业与环境流行病学. E-mail: 583179597@qq.com.*通信作者: 赵进顺（1960－）, 男, 山东海阳人, 教授, 主要研究方向: 预防医学、职业与环境医学. E-mail:zhaojinshun@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
