茶多酚对宁波近海水体8种常见重金属联合毒性的拮抗作用
PDF下载 (299)周 琪,苏 红,王亚飞,谷元亮,岳 霞,毛国传,邹宝波,赵进顺*.茶多酚对宁波近海水体8种常见重金属联合毒性的拮抗作用[J].宁波大学学报(理工版),2017,30(1):104-109.DOI:
ZHOU Qi,SU Hong,WANG Ya-fei,GU Yuan-liang,YUE Xia,MAO Guo-chuan,ZOU Bao-bo,ZHAO Jin-shun*.Antagonism effect of tea polyphenols to the joint toxicity of heavy metal mixtures on the offshore water in Ningbo area[J].Journal of Ningbo University(Natural Science & Engineering Edition),2017,30(1):104-109.DOI:
| Title: | Antagonism effect of tea polyphenols to the joint toxicity of heavy metal mixtures on the offshore water in Ningbo area |
| 作者: | 周 琪, 苏 红, 王亚飞, 谷元亮, 岳 霞, 毛国传, 邹宝波, 赵进顺* |
| Author(s): | ZHOU Qi, SU Hong, WANG Ya-fei, GU Yuan-liang, YUE Xia, MAO Guo-chuan, ZOU Bao-bo, ZHAO Jin-shun* |
| 关键词: | 重金属混合污染物; 联合毒性; 细胞周期; 氧化应激 |
| Keywords: | EGCG; mixed heavy metal-pollutants; joint toxicity; cell cycle; Oxidative stress |
| 分类号: | O651; R285 |
| 文献标识码: | A |
| 摘要: | 采用小鼠皮肤细胞株(JB6细胞)通过体外实验探索抗氧化剂茶多酚(Epigallocatechin gallate, EGCG)对8种常见重金属混合污染物的体外联合毒性是否具有拮抗作用, 为环境中多种重金属的联合毒性研究及评估和防治提供科学依据.基于文献对宁波养殖水体中8种常见重金属污染物的暴露情况进行评估, 确定联合暴露比例. 以此固定比例组成多元重金属混合物, 采用细胞学实验, 比较在重金属混合污染物暴露细胞的基础上加或不加10μmol·L-1抗氧化剂茶多酚对细胞生存活力、细胞周期、细胞内氧化应激的影响以及对Nrf2和HO-1蛋白的表达情况的影响. 结果显示: 重金属混合物可以降低细胞生存活力, 阻滞细胞周期, 而EGCG能够明显地缓解8种重金属混合污染物的细胞毒性, 提高细胞的生存率, 减轻细胞周期的阻滞, 使细胞内转录因子Nrf2和HO-1蛋白表达水平下调 |
| Abstract: | In this study, in vitro experiments were used to explore the antagonism effect and underlying mechanisms of antioxidant epigallocatechin gallate (EGCG) to the heavy metal mixture which is consisted of eight common heavy metal contaminants in the surface water of Ningbo area in China. These results will be useful as an important reference for the study on the prevention and treatment for multi-heavy metal pollutant mixture. Through the present situation of literature to understand the pollution of heavy metals in Ningbo city, and according to the literature and sea water quality standard, we can determine the concentration of eight common heavy metal ions in the surface water of Ningbo area for this experiment. At the same time, 10μmol·L-1 antioxidant polyphenols (Epigallocatechin gallate, EGCG) was tested to antagonize the toxicity effects of this mixture. Our results demonstrate that the mixture of heavy metal pollutants could arrest the JB6 cell cycle at S phase. Addition of EGCG in the mixture could inhibit the cell toxicity, relieve cell cycle arrest, and down-regulate the expressions of Nrf2 and HO-1. |
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| 备注/Memo: | 收稿日期: 2015?11?20. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（81273111）; 浙江省公益性技术应用研究计划项目（2015C33148）. 第一作者: 周琪（1992－）, 女, 江西九江人, 在读硕士研究生, 主要研究方向: 职业与环境流行病学. E-mail: zhouqi1992@yeah.net *通信作者: 赵进顺（1960－）, 男, 山东海阳人, 教授, 主要研究方向: 预防医学、职业与环境医学. E-mail: zhaojinshun@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
