小鼠股动脉及股静脉插管手术模型制备方法
PDF下载 (794)史宏博,RUTH Magaye,王 尊,赵进顺,邹宝波*.小鼠股动脉及股静脉插管手术模型制备方法[J].宁波大学学报(理工版),2012,25(03):71-74.DOI:
SHI Hong-bo,RUTH Magaye,WANG Zun,ZHAO Jin-shun,ZOU Bao-bo*.Introduction of a Micro-surgery Model Through Femoral Arterial and Venous Catheters in Mice[J].Journal of Ningbo University(Natural Science & Engineering Edition),2012,25(03):71-74.DOI:
| Title: | Introduction of a Micro-surgery Model Through Femoral Arterial and Venous Catheters in Mice |
| 作者: | 史宏博, RUTH Magaye, 王 尊, 赵进顺, 邹宝波* |
| Author(s): | SHI Hong-bo, RUTH Magaye, WANG Zun, ZHAO Jin-shun, ZOU Bao-bo* |
| 关键词: | 小鼠; 股动脉插管; 股静脉插管; 显微外科手术模型 |
| Keywords: | mice; femoral artery catheter; femoral vein catheter; micro-surgery model |
| 分类号: | R-332 |
| 文献标识码: | A |
| 摘要: | 介绍一种小鼠经股动脉及股静脉插管手术模型. 具体方法为: 小鼠经异氟烷麻醉后, 无菌 切开小鼠的颈部及腹股沟皮肤, 将已制备好的2 根J 形塑料导管植入皮下, 并从颈部引出, 经股 动脉及股静脉造口, 将J 端导管分别插入股动脉及股静脉内. 再将双股拧成麻花状的细铁丝固定 于颈部肌肉, 并与引出的塑料导管捆绑在一起. 固定细铁丝, 将塑料导管通过旋转输液阀与输液 泵相连. 手术后, 输液泵持续灌注20 u·mL-1 的肝素(12~15 μL·h-1), 以防止塑料导管内凝血. 手术 后1~2 d, 动物基本都能恢复到正常生理状况. 手术感染率低于6%, 7 d 动脉导管堵塞率低于5%. 这种经显微外科手术建立的动物模型, 可在动物清醒、自由活动状态下, 无痛苦地完成采血、给 药及血压等生命体征的系统监测, 被广泛应用于药理学、毒理学等各学科的实验研究中. |
| Abstract: | Through femoral arterial and venous catheters in mice, a micro-surgery model is introduced for sampling of arterial blood, and continuous intravenous infusion in the free-moving conscious mice. Some 8-to-10-week-old mice weighing 20-30 g were used in this study. After anesthesia by inhalation of isoflurane (5%), 1.5% of isoflurane was administered to the mouse during surgery. With the use of aseptic techniques, catheters were placed in the femoral artery for the measurement of arterial pressure and sampling of blood. Catheters placed in the femoral vein were used for infusions. The arterial and venous catheters, tunneled subcutaneously in the back of the neck in a lightweight tethering wire, were attached to a swivel device at the top of the cage. To prevent blood cruor, 20 u heparin (12-15 μL·h-1) was continuously infused through a doublechannel swivel. All mice fully recovered after surgery (1-2 d). Infection rate was less than 6%. The blockage rate of artery catheters was less than 5% 7 days after micro-surgery operation. The femoral arterial and venous cannular surgery is proven to be a dynamic, safe, as well as efficient micro-surgery model, which can be widely applied in pharmacological, toxicological, and clinical experimental studies. |
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| 备注/Memo: | 收稿日期: 2012?03?19. 宁波大学学报（理工版）网址: http://3xb.nbu.edu.cn 基金项目: 宁波市科研项目（SZX11073）; 宁波大学实验技术项目（SYJS-201101）. 第一作者: 史宏博（1988－）, 女, 内蒙古呼伦贝尔人, 在读硕士研究生, 主要研究方向: 工业毒物. E-mail: 583179597@qq.com *通讯作者: 邹宝波（1962－）, 女, 山东海阳人, 硕士/副主任技师, 主要研究方向: 实验动物手术技术. E-mail: zoubaobo@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
