一个水稻显性短根突变体的遗传分析和基因定位
PDF下载 (430)林 丽 1,陈文笔 2,庄飘飘 2,丁沃娜 2,朱世华 2*.一个水稻显性短根突变体的遗传分析和基因定位[J].宁波大学学报(理工版),2015,28(02):9-12.DOI:
LIN Li 1,CHEN Wen-bi 2,ZHUANG Piao-piao 2,DING Wo-na 2,ZHU Shi-hua 2*.Genetic Analysis Based Gene Mapping of a Dominant Short-Root Mutant in Rice[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(02):9-12.DOI:
| Title: | Genetic Analysis Based Gene Mapping of a Dominant Short-Root Mutant in Rice |
| 作者: | 林 丽 1, 陈文笔 2, 庄飘飘 2, 丁沃娜 2, 朱世华 2* |
| Author(s): | LIN Li 1, CHEN Wen-bi 2, ZHUANG Piao-piao 2, DING Wo-na 2, ZHU Shi-hua 2* |
| 关键词: | 水稻; 根系基因; 遗传分析; 基因定位 |
| Keywords: | rice (Oryza sativa L.); root gene; genetic analysis; gene mapping |
| 分类号: | S511 |
| 文献标识码: | A |
| 摘要: | 研究所用的水稻短根突变材料ksr3是从甲基磺酸乙酯诱变的籼稻品种Kasalath突变体库中筛选获得. 该突变体在苗期表现为主根、侧根和不定根明显变短且扭曲, 根毛异常浓密, 长度明显增加, 成熟期植株明显矮化. 遗传分析表明该短根突变性状受一对显性基因控制. 用突变体ksr3和Nipponbare杂交构建的F2群体进行基因定位, 将该基因定位于第7染色体上, 与STS标记S3569和S5817连锁, 在2个标记间发展4个新的STS标记, 最终将KSR3定位在S3702和S4046之间的312kb范围内. |
| Abstract: | A leaky rice mutant is singled out from an ethylmethane sulfonate (EMS)-mutagenized rice library based on its short root phenotype. The primary root, lateral root and crown root of ksr3 are found to be shorter in length, and accompanied with distortion. Opposite to what is expected, the root hairs of the mutant are thicker and longer. The plants are shorter than the wild type. The genetic analysis indicates that the mutation is controlled by a single dominant nuclear gene. To map KSR3, an F2 population is formed by crossing the mutant ksr3 with Nipponbare wild type. Using the published SSR markers and newly designed STS markers, KSR3 is first mapped between the microsatellite markers S3569 and S5817 on chromosome 7. Then, four new polymorphic sequence-tagged site markers are developed in the region. KSR3 is finally mapped between markers S3702 and S4046 with a physical distance of 312 kb. The finding is expected to help in cloning, functional study and breeding applications. |
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| 备注/Memo: | 收稿日期: 2014－03－07. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/基金项目: 国家自然科学基金（31371595）; 宁波大学胡岚优秀博士基金.第一作者: 林丽（1989－）, 女, 浙江丽水人, 在读硕士研究生, 主要研究方向: 植物分子生物学. E-mail: 734130460@qq.com*通信作者: 朱世华（1963－）, 男, 浙江宁波人, 博士/教授, 主要研究方向: 植物遗传学. E-mail: zhushihua@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
