一个水稻苗期黄化叶突变体基因的定位
PDF下载 (437)项显波 1,吴 晶 1,丁沃娜 2,朱世华 2*. 一个水稻苗期黄化叶突变体基因的定位[J].宁波大学学报(理工版),2015,28(03):5-8.DOI:
XIANG Xian-bo 1,WU Jing 1,DING Wo-na 2,ZHU Shi-hua 2*.
Molecular Mapping of a Rice Yellow Leaf Gene OsYL1
[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(03):5-8.DOI:| Title: | Molecular Mapping of a Rice Yellow Leaf Gene OsYL1 |
| 作者: | 项显波 1, 吴 晶 1, 丁沃娜 2, 朱世华 2* |
| Author(s): | XIANG Xian-bo 1, WU Jing 1, DING Wo-na 2, ZHU Shi-hua 2* |
| 关键词: | 水稻; 黄化叶突变体; 基因定位 |
| Keywords: | Oryza sative ; yellow leaf mutant; gene mapping |
| 分类号: | S511 |
| 文献标识码: | A |
| 摘要: | 通过筛选甲基磺酸乙酯(Ethyl Methane Sulfonate, EMS)诱变的籼稻Kasalath突变体库, 得到一个黄化叶突变体Osyl1(Oryza sativa yellow leaf 1), 在二叶期前该突变体叶色黄化, 后期突变体叶色逐渐恢复到野生型水平. 遗传分析显示Osyl1突变性状受一对隐性基因控制, 经图位克隆技术将OsYL1基因定位在水稻第3染色体上的STS(Sequence Tagged Site)标记S5362和S5845之间, 物理距离约为483kb, 在该区间内无已知黄化叶相关基因. 对OsYL1基因的定位, 为该基因的克隆打下基础. |
| Abstract: | Leaf color mutants are useful resource for rice genomics research and development of rice cultivars with high photosynthetic efficiency. In this research, a rice yellow leaf mutant is isolated from an EMS (ethyl methane sulfonate)-generated rice mutant library of Kasalath background, and Oryza sativa yellow leaf 1(Osyl1) is thus obtained. The Osyl1 shows a yellow leaf phenotype before the second leaf stage and later leaves gradually turn back to green similar to the wild type. Genetic analysis suggests that the phenotype of Osyl1 is controlled by a single recessive nuclear gene. By map-based cloning, the OsYL1 is mapped to a 483 kb region between the markers S5362 and S5845 on chromosome 3. No report on yellow leaf related gene has been found in this region. The result of this study is expected to provide useful reference for cloning and characterizing OsYL1. |
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| 备注/Memo: | 收稿日期: 2014?12?02. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家自然科学基金（31371595）; 宁波大学胡岚优秀博士基金. 第一作者: 项显波（1989－）, 男, 浙江温州人, 在读硕士研究生, 主要研究方向: 水稻分子生物学. E-mail: xiangxianbo@163.com *通信作者: 朱世华（1963－）, 男, 浙江宁波人, 博士/教授, 主要研究方向: 水稻分子生物学. E-mail: zhushihua@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
