低盐协迫对大黄鱼gh、igf-1、hsp90和pparβ基因表达变化的影响
PDF下载 (509)李明云,苗 亮,张 浩,王 涛,胡 谋,刘连亮,陈 炯.低盐协迫对大黄鱼gh、igf-1、hsp90和pparβ基因表达变化的影响[J].宁波大学学报(理工版),2015,28(04):1-6.DOI:
LI Ming-yun,MIAO Liang,ZHANG Hao,WANG Tao,HU Mou,LIU Lian-liang,CHEN Jiong.Effects of Low Salt Stress on Expression of gh, igf-1, hsp90 and pparβ Gene in Pseudosciaena crocea[J].Journal of Ningbo University(Natural Science & Engineering Edition),2015,28(04):1-6.DOI:
| Title: | Effects of Low Salt Stress on Expression of gh, igf-1, hsp90 and pparβ Gene in Pseudosciaena crocea |
| 作者: | 李明云, 苗 亮, 张 浩, 王 涛, 胡 谋, 刘连亮, 陈 炯 |
| Author(s): | LI Ming-yun, MIAO Liang, ZHANG Hao, WANG Tao, HU Mou, LIU Lian-liang, CHEN Jiong |
| 关键词: | 大黄鱼; 低盐协迫; 基因表达 |
| Keywords: | Pseudosciaena crocea; low salt stress; gene expression |
| 分类号: | Q785; S965 |
| 文献标识码: | A |
| 摘要: | 为了解大黄鱼在低盐胁迫下相关基因的表达变化, 采用实时荧光定量PCR技术检测了正常盐度25和降低盐度后3个节点(盐度8、盐度3和盐度1.5)鳃、肾、肝、心、脾、肌肉、脑和肠8组织中gh、igf-1、hsp90和pparβ基因mRNA的表达量变化. 研究表明: 低盐胁迫可显著影响大黄鱼中这4个基因的表达量. gh基因在盐度1.5节点除心脏外各组织中表达量均显著高于正常盐度(P<0.05), 其中脾的升幅最大; 盐度8和盐度3节点, 均是肝中gh表达量升高幅度最大, 分别为正常盐度的5.21和3.11倍. igf-1基因在盐度1.5节点除肌肉和脑外表达量均显著上调(P<0.05), 鳃中表达量最高, 为正常盐度的5.28倍, 其次为肾(3.05倍)和肝(2.20倍). hsp90基因在盐度8节点时心和肝中的表达量显著升高(P<0.05); 盐度1.5节点心中表达量显著降低(P<0.05), 而在其他组织中的表达量均显著升高(P<0.05). 在盐度8节点pparβ基因在心、肌肉、肝和脾中的表达量均显著高于其他3个盐度点(P<0.05); 盐度1.5节点鳃、脑、肾和肠中的表达量均显著升高(P<0.05), 分别为正常盐度时的9.04、7.36、3.39和3.19倍. 上述结果可为深入研究大黄鱼应对低渗环境的分子调控机制提供基础资料. |
| Abstract: | In order to investigate the gene expression changes responding to low salt stress, we detected the expression level of gh, igf-1, hsp90 and pparβ gene in different tissues of large yellow crocea (Pseudosciaena crocea) by real-time quantitative PCR. The samples were collected at normal salinity (25) and three low salt nodes (8, 3 and 1.5). The result shows that the expression of these four genes is significantly altered under low salt stress. At salinity 1.5 node, the expression level of gh rises significantly (P<0.05) in all tissues except heart, and the most significant increase of amplitude is observed in spleen. Among all the sampled tissues, liver shows the most notable increase of amplitude of gh expression both at salinity 8 and 3 nodes, and the expression level is 5.21 and 3.11 times the normal salinity, respectively. The expression of igf-1 mRNA goes up significantly (P<0.05) in all tissues at salinity 1.5 node, except muscle and brain. At salinity 1.5 node, the expression level of igf-1 is found in gill, which turns out to be 5.28 fold to normal salinity, and followed by kidney (3.05 fold) and liver (2.20 fold). The expression level of hsp90 rises significantly (P<0.05) in heart and liver at salinity 8 node. At salinity 1.5 node, the expression of hsp90 is declined significantly (P<0.05) in heart, while all the other tissues climbs significantly (P<0.05). At salinity 8 node, the expression level of pparβ in heart, muscle, liver and spleen are all significantly higher than the other three salinities (P<0.05). At salinity 1.5 node, the expression of pparβ in gill, brain, kidney and gut all raised significantly (P<0.05), and are 9.04, 7.36, 3.39 and 3.19 fold to normal salinity. These results are expected to provide fundamental data for further research on the molecular mechanism of response to low salt stress in Pseudosciaena crocea. |
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| 备注/Memo: | 收稿日期: 2015-03-17. 宁波大学学报（理工版）网址: http://journallg.nbu.edu.cn/ 基金项目: 国家高技术研究发展计划（2012AA10A403-4）; 浙江省重大科技专项优先主题（2009C12077, 2012C12907-8）; 宁波市重大项目（2008C3005）. 第一作者: 李明云（1942－）, 男, 浙江舟山人, 教授, 主要研究方向: 鱼类遗传育种与种质保护. E-mail: limingyun@nbu.edu.cn 宁波大学学报（理工版）网址：http://journallg.nbu.edu.cn/ |
